Design and analyze an experiment to test whether cells of the slime mold Dictyostelium discoideum aggregate with kin

Consider the slime mold Dictyostelium discoideum as an experimental model for the evolution of social behavior. This organism is discussed in your text in Chapter 12 and on pp. 607-608 and in the study guide for the multicellularity/individuality lecures. Watch videos of slime mold aggregation here: https://www.youtube.com/watch?v=tpdIvlSochk . Copy the link in your browser if it doesn't work by clicking on it.

Kin selection theory (inclusive fitness and Hamilton’s rule) provides the basis for predicting situations in which individuals will give up reproduction to help others reproduce. Note that the stalk cells in D. discoideum are giving up reproduction and helping the spore cells to reproduce. Note that slime mold cells aggregate to form fruiting bodies when they are starved. One prediction of kin selection theory is that individuals will aggregate and cooperate preferentially with genetic relatives. Using the steps below, design an experiment to assess whether slime mold cells aggregate preferentially with genetic relatives.

Assume in your experiment that you have two genetic clones that are unrelated to one another: genetic clone A and genetic clone B. In other words, the cells in genetic clone A are genetically identical to each other but unrelated to the cells in clone B. Likewise, the cells in genetic clone B are genetically identical to each other but unrelated to the cells in clone A. Imagine you can inject and thereby label cells with colored dyes: say a red dye and a yellow dye. Again, the hypothesis you wish to test with your experiment is that slime mold cells aggregate preferentially with relatives.

Look ahead to the last question in this section that gives a template for the bar graph in which the results of your experiment must be displayed. This should help you design your experiment. Remember to label your y-axis.

Hand drawn diagrams are fine so long as they are clear. Take a photo and upload it. Photos must be of high quality and readable.

Transcribed Image Text:C) What is your predictor (dependent) variable and how will you measure it? D) Provide a bar graph (sometimes called a bar chart) using the template below of the predicted results of your experiment, if the hypothesis that aggregation is preferentially with genetic relatives, is to be accepted. On the y-axis is your dependent variable, make sure to label it using your answer to the previous question. Make sure the predicted difference between the treatment and control is clear. 100 50 50 0 Treatment Control
Transcribed Image Text:IF YOU CAN'T ANSWER ALL THAT'S EINE I WOULD JUST NEED THE FORST AND SECOND IF POSSIBLE... THANKS A) Provide a simple illustration of the design of your control experiment. Recall, the results of the "control" experiment provides a baseline to which you compare the results of your "treatment" experiment. Use the insert image button to upload a single image of your control experiment. Only a single image may be uploaded. Begin your experimental design with something similar to the diagram below. In this diagram the circles correspond to individual cells belonging to either clone A or clone B. The cells in clone A are all genetically identical but unrelated to the cells in clone B. Likewise, the cells in clone B are all genetically identical but unrelated to the cells in clone A. The last step of your experimental design should be to starve the cells so they aggregate and then analyze the aggregates in the manner you give. Clone A Clone B B) Provide a simple illustration of the design of your treatment experiment. Only a single image may be uploaded. Provide a simple illustration of the design of your control experiment. Recall, the results of the "control" experiment provides a baseline to which you compare the results of your "treatment" experiment. Use the insert image button to upload a single image of your control experiment. Only a single image may be uploaded. Begin your experimental design with something similar to the diagram below. In this diagram the circles correspond to individual cells belonging to either clone A or clone B. The cells in clone A are all genetically identical but unrelated to the cells in clone B. Likewise, the cells in clone B are all genetically identical but unrelated to the cells in clone A. The last step of your experimental design should be to starve the cells so they aggregate and then analyze the aggregates in the manner you give. Clone A Clone B

Question

Design and analyze an experiment to test whether cells of the slime mold Dictyostelium discoideum aggregate with kin

Consider the slime mold Dictyostelium discoideum as an experimental model for the evolution of social behavior. This organism is discussed in your text in Chapter 12 and on pp. 607-608 and in the study guide for the multicellularity/individuality lecures. Watch videos of slime mold aggregation here: https://www.youtube.com/watch?v=tpdIvlSochk . Copy the link in your browser if it doesn't work by clicking on it.

Kin selection theory (inclusive fitness and Hamilton’s rule) provides the basis for predicting situations in which individuals will give up reproduction to help others reproduce. Note that the stalk cells in D. discoideum are giving up reproduction and helping the spore cells to reproduce. Note that slime mold cells aggregate to form fruiting bodies when they are starved. One prediction of kin selection theory is that individuals will aggregate and cooperate preferentially with genetic relatives. Using the steps below, design an experiment to assess whether slime mold cells aggregate preferentially with genetic relatives.

Assume in your experiment that you have two genetic clones that are unrelated to one another: genetic clone A and genetic clone B. In other words, the cells in genetic clone A are genetically identical to each other but unrelated to the cells in clone B. Likewise, the cells in genetic clone B are genetically identical to each other but unrelated to the cells in clone A. Imagine you can inject and thereby label cells with colored dyes: say a red dye and a yellow dye. Again, the hypothesis you wish to test with your experiment is that slime mold cells aggregate preferentially with relatives.

Look ahead to the last question in this section that gives a template for the bar graph in which the results of your experiment must be displayed. This should help you design your experiment. Remember to label your y-axis.

Hand drawn diagrams are fine so long as they are clear. Take a photo and upload it. Photos must be of high quality and readable.

C)
What is your predictor (dependent) variable and how will you measure it?
D)
Provide a bar graph (sometimes called a bar chart) using the template below of the
predicted results of your experiment, if the hypothesis that aggregation is
preferentially with genetic relatives, is to be accepted. On the y-axis is your
dependent variable, make sure to label it using your answer to the previous question.
Make sure the predicted difference between the treatment and control is clear.
100
50
50
0
Treatment
Control

IF YOU CAN'T ANSWER ALL THAT'S EINE I WOULD JUST NEED THE FORST AND
SECOND IF POSSIBLE... THANKS
A)
Provide a simple illustration of the design of your control experiment. Recall, the
results of the "control" experiment provides a baseline to which you compare the
results of your "treatment" experiment. Use the insert image button to upload a
single image of your control experiment. Only a single image may be uploaded. Begin
your experimental design with something similar to the diagram below. In this
diagram the circles correspond to individual cells belonging to either clone A or clone
B. The cells in clone A are all genetically identical but unrelated to the cells in clone
B. Likewise, the cells in clone B are all genetically identical but unrelated to the cells
in clone A. The last step of your experimental design should be to starve the cells so
they aggregate and then analyze the aggregates in the manner you give.
Clone A
Clone B
B)
Provide a simple illustration of the design of your treatment experiment. Only a
single image may be uploaded.
Provide a simple illustration of the design of your control experiment. Recall, the
results of the "control" experiment provides a baseline to which you compare the
results of your "treatment" experiment. Use the insert image button to upload a
single image of your control experiment. Only a single image may be uploaded. Begin
your experimental design with something similar to the diagram below. In this
diagram the circles correspond to individual cells belonging to either clone A or clone
B. The cells in clone A are all genetically identical but unrelated to the cells in clone
B. Likewise, the cells in clone B are all genetically identical but unrelated to the cells
in clone A. The last step of your experimental design should be to starve the cells so
they aggregate and then analyze the aggregates in the manner you give.
Clone A
Clone B

Accepted Answer

A.) Design of Control ExperimentFor a control experiment with Dictyostelium discoideum, you would…