If there are 103 cells per mL at the middle of log phase, and the generation time of the cells is 30 minutes, how many cells will there be 2 hours later? a) 2 x 103 b) 4 x 103 c) 8 x 103 d) 1.6 x 104 e) 1 x 107
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If there are 103 cells per mL at the middle of log phase, and
the generation time of the cells is 30 minutes, how many cells
will there be 2 hours later?
a) 2 x 103
b) 4 x 103
c) 8 x 103
d) 1.6 x 104
e) 1 x 107
Step by step
Solved in 3 steps with 2 images
- Q1. The total area of a haemocytometer chamber is a) 1 mm² b) 15 mm² c) 16 mm² d) 9 mm² **********...In step 3 of today's protocol, it mentions that the incubation step with the P2 buffer should not be allowed to proceed for more than 5 minutes. a) What is the chemical composition and concentration (not mass) of each component of the P2 buffer?b) c) What is the purpose of adding the P2 buffer? What would be the major problem if this step is allowed to proceed for a long period of time?A 26y female patient suffered from Ⅱ° and Ⅲ° burn of bilateral upper limbs, trunk and perineum,and her body weight was 60kg, according to the experience formula, the volume of crystalloid and colloid needed in the first 24h should be() A) 2000ml B) 4000ml C) 4050ml D) 5000ml E) 6050ml
- Order: 1000 mL D5NS with 15 mEq KCL IV to run over 8 hoursa) ML/hr b) Macro drop (20 gtt/mL) c) Micro drip (50 gtt/mL)USING THE CONVERSION TABLE, MAKE THE FOLLOWING CALCULATIONS. 1) 26 lb= 2) 15 kg = 3) 3 oz =_ 4) 300g= 5) 4 Tbsp=_ 6) 800 mL = 7) 64 oz- 8) 1,800µg = 9) 800L= 10) 6mL= kg lb _mg mL cup. lb ML gotas. (Ctrl) - H50 g of food is homogenized in 450 mL of water. From this, 1 mL is added to 29 mL of water, and then a 1:90 dilution is made from this, and then 1 mL from this is added to 99 mL of water, and then 100 uL of this last dilution is plated and after incubation 164 colonies are counted.a) What was the overall dilution factor?b) What was the total CFU/g of the original sample?
- It is possible to measure the number of cells in a broth by measuring the amount of light that goes through the tube. (Spectrophometric analysis). This method measures both live and dead cells. If one measures the same sample by spectrophometric analysis and the spread plate method than a) The CFU>the number calculated by the spectrophometric analysis b) The CFU = the number calculated spectrophometric analysis c) The CFUWhich of the following statements is correct about column chromatography? a) Resolution improves as column length increases b) Mobile phase is a porous solid material kept in the column with appropriate chemical characteristics c) Stationary phase is a buffered solution that percolates through the mobile phase d) Large proteins exit the column faster than tiny proteinsDuring reflux, what is the most suitable heater setting? How long the liquid needs to be boiled at this heat setting? options: A) Heater setting = 9, Continue heating till 2-3 mL of the liquid is left behind in the boiling flask. B) Heat setting = 9 for 30-45 minutes C) Heat setting = 6 for 30-45 minutes D) Heat setting = 5 for 45-60 minutesWhen the aspirin is tested to see if it breaks down more efficiently in HCI or NaOH; a) why is a control test done using salicylic acid? b) what is the purpose of the FeCl3 in thisaspirin test?Regarding the use of animal cells in culture, it is correct to state that: * (a) Low cost culture media composed of mineral salts and amino acids are used (b) Cells must be kept in semi-solid media, allowing them to come into contact with the atmosphere rich in carbon dioxide. (c) Greenhouses with controlled temperature and atmosphere composed predominantly of carbon monoxide are used (d) On an industrial scale, culture media supplemented with serum of animal origin are used, which makes them media of indefinite composition (e) Culture media must not be sterilized by autoclaving, preferably filtration and/or ionizing radiation methods are usedYou are observing the results from a standard plate count and you observe 53 CFUS. To make the plate count, you transferred 1 mL of a 10-4 dilution. How many CFU/mL are in the original sample? 1) 5.3 x 10-4 O 2) 5.3 x 103 O 3) 5.3 x 104 4) 5.3 x 105 O 5) 5.3 x 10-5SEE MORE QUESTIONS