If 3 ml of culture is diluted by adding 9 ml of water and the absorbancy reading of the diluted culture is 0.082, what is the actual absorbancy?
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- 10 If 3 ml of culture is diluted by adding 9 ml of water and the absorbancy reading of the diluted culture is 0.082, what is the actual absorbancy? 11 If 0.1 ml of a 10-3 dilution of a culture is plated out and 65 colonies appear after incubation, how many CFU/ml were in the undiluted culture?11 If 0.1 ml of a 10-3 dilution of a culture is plated out and 65 colonies appear after incubation, how many CFU/ml were in the undiluted culture?16. Your Excel data graph for Serial Dilution lab:** 16a. Include a clear and labeled graph of DF (x axis) versus Absorbance (y axis). See box above for expectations and review video tutorial of this Excel work. Do not graph two of your data points: your blank or your Cuvette #1 (DF=1). 1 Q A Multiple Styles W S X 3 E D $ 4 To C R F % 5 I T V U ^ 6 G Y 7 H U 8 J BN S ( 9 K M X
- Time point (min) Absorbance of culture at 660nm Approximate cell concentration Approximate # cells in 1mL extract 0 0.298 1.49 x 108 cells/mL 1.49 x 108 cells 10 0.316 1.58 x 108 cells/mL 1.58 x 108 cells 20 0.374 1.87 x 108 cells/mL 1.87 x 108 cells 30 0.429 2.145 x 108 cells/mL 2.145 x 108 cells 40 0.512 2.56 x 108 cells/mL 2.56 x 108 cells 50 0.544 2.72 x 108 cells/mL 2.72 x 108 cells 60 0.607 3.035 x 108 cells/mL 3.035 x 108 cells a. Using these data, prepare a growth curve of this strain ofEscherichia coli (E. coli).b. Estimate the doubling time for this strain of E. Coli. Clearly showhow you estimated this value from the empirical data presented.2 if there is a stock solution of 2000 (ug/ml) , is it possible to use SERIAL DILUTION to generate the following 8 tubes with their concentrations? A 2000 (ug/ml) B 1500 (ug/ml) C 1000 (ug/ml) D 750 (ug/ml) E 500 (ug/ml) F 250 (ug/ml) G 125 (ug/ml) H 0 (ug/ml)Q1.5 ... If the equation produces from data is y = 0.300x-0.857 and the absorbance for the unknown is 0.429. What would be the concentration of the unknown. Give the answer with 3 decimal places. Type your numeric answer and submit Unanswered a Save Q1.9 Fill in the Blanks Type your answers in all of the blanks and submit The microplate reader is used to take measurements of Type your answer here and Type your answer here
- DILUTION COLONY COUNT CFU/mL 1:106 155000 1:107 15500 1:108 1550 1:109 155 Given these values how would I fill in the rest of this serial dilution table? Also, what would be a 1:1 CFU/mL value based on this table?1&3/4 tbs po BID x 7 days #QS How many milliliters should the pharmacy dispense?How much of 10,000x SYBR safe would you add to 50 ml to make a final concentration of 1x? V1= 0.005mL B.How will you set up the serial dilution? How many tubes do you need? What is the concentration in each? How much LB will you add to each tube? What volume of cells will you add?
- Solution Absorbance mg/ml aspirin Standard solution - 1.6 mg/mL A 0.638 0.08 mg/mL B 0.504 0.064 mg/mL C 0.376 0.048 mg/mL D 0.259 0.032 mg/mL E 0.126 0.016 mg/mL A = -log T where T = %T ÷ 100 Construct a callibration curve using the above data. Absorbance should be on the vertical axis and "mg/mL of acetylsalicylic acid" on the horizontal axis. The line should go through the origin. Using the data provided, the graph you have generated, and the procedure that was used to generate the solutions which were examined by spectroscopy, calculate the amount of acetylsalicylic acid per tablet. Commercial tablet 1 labelled as 100 mg enteric coated Absorbance = 0.16 Commercial tablet 2 labelled as 300 mg Absorbance = 0.45 Student prepared tablet from practical 5 Absorbance = 0.19 Using the data provided, the graph you have generated, and the procedure that was used…11 of 11 What volume (in µL to 1 decimal place) of stock SybrSafe solution (3.4 mg/mL) would you add to 120 mL of agarose solution to give a final concentration of 0.4 pg/mL? Answer: Submit < Previous Question Assessment Navigator Questionmark Ondemand licensed to University of Dundee DII DD 80 000 000 F10 F8 F9 F7 esc F5 F6 F3 F4 F1 F2 24 ... 6 7 * 002. For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. How much of the diluent must be added to tube 2 if 200uL Red cell suspension is added? Show pertinent solutions. What are the initial concentrations used for tubes 3, 5, and 6? Show pertinent solutions.