HPO, 10- | HPO, pH pK +1 8- pK +1- (H¿PO]] = [HPO,"] pK = 7.2 Buffer pH 7- 7.2 = pK,- pK, -1- region 6- PK – 1 Inflection point H„PO, H,PO, 4 50 100 % H,PO, in solution % HPO in solution 100 90 50 10 Relative abundance (%) O 10 50 90 100 Н.РО] in excess НРО in excess The titration curve of H,PO, showing the buffer region for the H2PO7/HPO pair. Relative abundance of H,PO, and HPO". FIGURE 2.17 The relationship between the titration curve and buffering action in H,PO,.
HPO, 10- | HPO, pH pK +1 8- pK +1- (H¿PO]] = [HPO,"] pK = 7.2 Buffer pH 7- 7.2 = pK,- pK, -1- region 6- PK – 1 Inflection point H„PO, H,PO, 4 50 100 % H,PO, in solution % HPO in solution 100 90 50 10 Relative abundance (%) O 10 50 90 100 Н.РО] in excess НРО in excess The titration curve of H,PO, showing the buffer region for the H2PO7/HPO pair. Relative abundance of H,PO, and HPO". FIGURE 2.17 The relationship between the titration curve and buffering action in H,PO,.
Chapter7: Statistical Data Treatment And Evaluation
Section: Chapter Questions
Problem 7.26QAP
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Look at Figure 2.17. If you did this titration using TRIS instead of phosphate, how would the titration curve look compared to the figure? Explain.
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