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A: Answer
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How will you determine whether the antibiotics kill the organism or only inhibit its growth
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- Some bacteria produce the enzyme penicillinase, which breaks the beta-lactam ring in the penicillin molecule and converts it into penicilloic acid, which is harmless to bacteria. This is an example of which of the following mechanisms of antibiotic resistance? which option below is the answer? Destruction or inactivation of the antibiotic by the microbe Preventing penetration to the target site within the microbe Alteration of the drug’s target site(s) within the microbe None of the other four answers are correct Rapid ejection (pumping) of the drug out of the microbial cellHow is UV radiation a good type of control mechanism against microbial growth? Explain what happens to the microbe and effects this control causes.Why is it important to use sterile practices when working with microorganisms?
- This diagram shows the filter paper method used to evaluate the inhibitory effect of chemical agents, heavy metals, and antibiotics on bacterial growth. A culture of a test bacterium is spread uniformly over the surface of an agar plate. Small filter paper discs containing the material to be tested are then placed on the surface of the medium. A disc that has been soaked in sterile distilled water is sometimes added as a control. After incubation, a lawn (film of growth) will cover the plate, but a clear zone will surround those discs that contain an inhibitory compound. The size of the zone reflects several factors, one of which is the effectiveness of the inhibitory agent. What are two other factors that might affect the size of the zone of inhibition? What is the purpose of the control disc? If a clear area were apparent around the control disc, how would you interpret the observation?One of the following is INCORRECT about Spread plate a-Allow aerobic bacteria to grow b-The sample is spread on agar surface by a sterile glass spreader c-Only Surface colonies emerge after incubation d-Exclude Heat-sensitive bacteria.Why is it recommended to add pre- and probiotics to antibiotic treatment?
- In a bacterial culture on an agar plate, what is a zone of inhibition? An area where the growth of bacteria is increased compared to the rest of the agar plate An area where the growth of bacteria is prevented or reduced compared to the rest of the agar plate An area where the growth of bacteria is not affected compared to the rest of the agar plate An area where the the agar becomes contaminated with moldYou found a bright blue growth on a tomato you had left on your kitchen counter. You’ve never seen a microorganism that color, and you want to know what it is. You take the tomato into the lab, sterilize an inoculating loop, transfer a sample from the tomato to a Nutrient Agar Petri dish, and incubate it at 37°C. But when you come back two days later, your Petri dish is empty. Why might this be? Be specific about what might have happened. How would you test your hypothesis?A class of 15 students (8 males and 7 females) will need to culture an unknown bacteria for a specific activity where 5 nutrient agar pates per female student and 3 agar slants per males student are needed to prepare. agarplate: 25mL Agar slant: 10mL Broth tube: 8mL Nutrient Agar: Yeast extract: 2g/L peptone: 5g/L Sodium chloride: 5g/L Agar powder: 15g/L
- Why is it important for a microbiology laboratory technician to use aseptic technique?You are working for the CDC studying outbreaks of foodborne infectious disease. Your latest case is an incidence of food poisoning in a fast food restaurant. 5 people have been hospitalized for food poisoning, so you are working with that hospital to identify the source of the outbreak. Hospital clinicians have taken samples from each of the 5 patients for culturing. Each bacterial culture grew one likely microbe, each labelled P1, P2, P3, P4, and P5. All 5 isolates appear to have the same colony morphology on the plates, and all appear to be Gram-negative rods 1 micron wide and 3 microns long under the microscope. In addition, all 5 isolates appear to perform the same on an API 20E test strip that tests bacteria for 20 biochemical abilities. No other testing has been done yet. How would you best describe your set of isolates at this moment? O Each isolate is the same strain, of a different species O Each isolate is a different strain, of different species O Each isolate is a different…In the laboratory, autoclaving is required to prepare media for the growth of microorganism. What type of method of control does this involve? Dry Heat Moist Heat Chemical Radiation All of the options