How to reduce or eliminate aerial contamination inside the laboratory?
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How to reduce or eliminate aerial contamination inside the laboratory?
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- Aseptic technique refers to A) the microbial inoculum placed into a test tube or onto a Petri plate. B) a series of practices to avoid contamination. C) the autoclave and other sterilizing procedures. D) cleanliness in the laboratory.In a tabulated form, differentiate the physical and chemical methods of sterilization.Identify the principles and procedures related to disinfection and sterilization.
- Why is it important to become familiar with the microbiology laboratory's apparatus and equipment?What is the purpose of degerming? Does it completely eliminate microbes? What are some factors that alter the effectiveness of a disinfectant?In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropper
- What are the advantages and disadvantages of plate count technique over other methods of quantifying microbial populations?What is the difference between a disinfectant and an antiseptic? Which is most effective at removing microbes from a product: sanitization, degerming, or sterilization? ExplainWhat is an alternative to autoclaving for sterilizing heat-sensitive materials?
- What will pose an ethical issue in these procedures are performed - mutilation, sterilization?In paragraph form (about 2-3 long paragraphs), how would you characterize and identify antimicrobial agents from root samples using chromatography and electrophoresis? Please be in full details.Which techniques may be used for viable counting methods of microbial measurements? Explain.