How to calculate the percentage of phage that was filtered using a 0.45 um filter? PFU rep 1- 121 PFU PFU rep 2- 102 PFU Volume stock plated- 0.1 mL Average concentration= 5.6 x 109 PFU/mL.
Q: Define the different types of Types of Cloning Vectors ?
A: A cloning vector is a small piece of DNA, into which a foreign DNA fragment can be inserted for mult...
Q: What general measure/s can you recommend in order to prevent the majority of the parasitic and funga...
A: a) Providing water sources free of pathogens. b) Protection from the transfer of pathogens. c) Dis...
Q: What causes the dark feet, ears, and nose of Himalayan rabbits? sex linkage and Barr bodies A gene i...
A: Introduction :- The Himalayan rabbit is a rare medium-sized rabbit that is sometimes confused with t...
Q: Show the diagram for DNA replication of prokaryotes.
A: Eukaryotic DNA replication occurs through multiple replication origins and each origin of replicatio...
Q: What cell type contributes to the formation of a foreign body giant cell? Draw the cell(s) before an...
A: Introduction: A macrophage is a type of white blood cell (WBC) present within the immune system of t...
Q: Describe and explain the epidemiological evidence supporting the view that cancer develops through a...
A: Note- as we are allowed to do only one question at a time. Kindly repost the other question separate...
Q: 1. Q. How are plocoid and placeloid corallites the same? 2. Q. How are plocoid and placeloid coralli...
A: Introduction: A corallite is a skeletal cup created by a single stony coral polyp, into which the po...
Q: There were ape smugglers wreaking havoc on populations of orangutans, chimpanzees, and bonobos. What...
A: Endangered ape species include orangutans, chimpanzees and bonobos Chimpanzees and bonobos are close...
Q: 2. A hiker got lost while hiking in the Mohave desert in Arizona. When she was found by a search gro...
A: Distilled water is pure water with no solutes. present in it. Water is absorbed by the cells through...
Q: Which animal is the most distant relative (less related) to the other three? Zebra Animalia Rhinocer...
A: Classification is the way to categorize organisms depending upon their characters. classifications ...
Q: How do the life cycles of seedless plants and seed plants differ? In what fundamental ways are they ...
A: Introduction Plants are mostly photosynthetic eukaryotes belonging to the Plantae kingdom. Historica...
Q: If life were found on another planet, do you think that itwould have the same genetic code? Justify ...
A:
Q: Explain Darwin's theory of descent modification.
A: Introduction Evolution is the process of a species' features changing over numerous generations thro...
Q: Consider the genes that specify the structure of hemoglobin. Arrange the following events in the mos...
A: Hemoglobin is present in red blood cells.Hemoglobin is made up of heme and oxygen molecule and respo...
Q: Complete dominance at both gene pairs, however, when either gene is dominant, it hides the effect of...
A: Epistasis When a gene effect the expression of non allelic gene then this phenomenon is known as ep...
Q: Which of the following statements regarding loss of function and gain of function mutations is true?...
A: Mutations are alterations in the DNA sequence of an organism. Small changes, such as adding or remov...
Q: Infoldings of the plasma membrane into the cytoplasm ofsome ancestral cells may have evolved into th...
A: The correct answer of the given question is d. Infoldings of the plasma membrane into the cytoplasm ...
Q: A condition wherein there is a blending of traits on the third phenotype. multiple alleles lethal ge...
A: The debunked hypothesis that passing down features from two parents results in kids with attributes ...
Q: What Are the Differences Between Eukaryotic and Prokaryotic Cells?
A: Introduction In this question we will write the differences between Eukaryotic a d Prokaryotic cells...
Q: The host cell in the endosymbiosis that produced the firstchloroplasts was a(n) ________.a. early eu...
A: Introduction Eukaryotes are organisms with a nucleus encased within a nuclear envelope in their cell...
Q: What levels caused the diversification event?
A: Diversification is a phenomena in which good number of new species formed by making changes in the ...
Q: What is a chromatid
A: The cell consists of genetic material in its nucleus, this genetic material (DNA) is present in the ...
Q: G1 of interphase
A: The stages of interphase are: G1 (Gap 1), in which the cell grows and functions normally .During thi...
Q: Get SNR-dependent graphs of BER and interruption probabilities for each state by doing receiver dive...
A: Get SNR-dependent graphs of BER and interruption probabilities for each state by doing receiver dive...
Q: What accounts for the difference in how bonobos and chimps respond to strangers? Why can't chimps ge...
A: Introduction: Evolution is the change in the inherited characteristics of biological populations ove...
Q: 2. Male dragonflies of one species are unable to grasp the abdomen of female dragonflies of a differ...
A: Note: As per Bartleby Guidelines For Remaining Answers Please Repost The Question. Introduction: Rep...
Q: What type of bond (name other than covalent) that holds the monomers of carbohydrates together?
A: A carbohydrate is a biomolecule made up of carbon (C), hydrogen (H), and oxygen (O) atoms, usually i...
Q: what is the present enzyme in making kabocha squash soup?
A: Kobocha squash soup is Japanese variety of winter squash.its Having heavy and thick-fleshed texture ...
Q: Draw the metaphase 1 and anaphase 1.
A: Given,There are 9 pairs of chromosomes which undergoes mitosis and meiosis 1. After mitosis,2 cells ...
Q: One feature that virtually all plasmid vectors have incommon is the polylinker (also called a multip...
A: A restriction site is that 6 – 8 DNA sequence that is recognized and bounded by a particular restric...
Q: Why restriction enzymes are used ?
A: Introduction: Restriction enzymes are endonucleases that cleaves the DNA at specific sites. They are...
Q: How can we differentiate so many different foods if we can only taste four flavors on our tongue: sw...
A: The taste buds in human tongue has tiny and simple hair like structures called as microvilli. This s...
Q: Chapter 12: DNA Organization in Chromosomes 1. Describe the types genetic material that make up bact...
A: 1. Type of genetic material that makes up bacterial chromosomes - Most bacteria has a haploid genome...
Q: 1. One individual has blood type AB positive, while another individual has blood type O positive, an...
A: Sickle cell anaemia is an autosome linked recessive trait that can be transmitted from parents to t...
Q: In the DNA extraction. What is the purpose of squashing the fruit?
A: DNA is a double-stranded molecule kept together by weak links between base pairs of nucleotides. It ...
Q: Cytochrome B6F pumps protons (H+ ions) from: The lumen into the stroma The stroma into the lumen The...
A: Introduction: Electron transport chain or system is the series of electron carriers, enzyme and cyto...
Q: Contrast the algae, mosses, ferns, gymnosperms, and angiosperms with respect to their dependence on ...
A: Different categories of plants include algae, pteridophytes, angiosperms, bryophytes and gymnosperms...
Q: Consider the mechanism of net NaCl secretion across an epithelium shown below: b) What is the drivi...
A: Introduction: Sodium potassium pump is a type of coupled or co-transport in which transport of one i...
Q: 10. About 70% of Americans get a bitter taste form the substance called phenylthiocarbamide (PTC). I...
A: The alleles are the alternative forms of a gene that are located on the same locus of a homologous c...
Q: At which rank does the zebra split from the rhinoceros? Zebra Animalia Rhinoceros Animalia Chordata ...
A: Classification: it is defined as the arrangement of taxa into a orderly manner. It is not a one step...
Q: It is the hormone that releases progesterone a. estrogen b. luteinizing c. progesterone d. inhib...
A: Hormones are the messengers that are secreted by various glands of the body and transport through bl...
Q: Two chromosomes have the ff. order of genes: Normal A B centromere C D ...
A: a) Paracentric inversion: it's the type of inversion that doesn't involve the exchange of genes arou...
Q: Evolution of ________ increased the energy availableto cells, making a larger genome and processes s...
A: Introduction Evolution is the process of a species' features changing over numerous generations thro...
Q: After fertilization, the _______________ develop(s) into a fruit and the _______________ develop(s) ...
A: Fertilization in plants (flowering) is called Double fertilization in which there are two sperm cell...
Q: Red-green colorblindness is an X-linked trait. From which parent can a SON inherit the red-green col...
A:
Q: What characters distinguish fungi from other organisms?
A: Introduction The kingdom Fungi, which includes yeasts, rusts, smuts, mildews, moulds, and mushrooms,...
Q: The probability of having an individual recessive for all gene pairs from the cross Aabb x aaBb is O...
A: The detailed solution with Punnett square is discussed in the step 2
Q: The genotype AaBbCCDd have how many possible types of gametes? O 16 0 8 0 4 6
A: Genotype : the genetic makeup of the individual for a specific character or trait is known as genoty...
Q: WHAT IS THE ECONOMICS IMPORTANCE OF PENICILLUM DIGITATUM? WITH SOURCE PLEASE.
A: Answer
Q: water is the answer but can you explain why it is? In Miller’s original experiments on chemical evo...
A: ANSWER;- Water is known as a universal solvent because more substances dissolve in water than in any...
How to calculate the percentage of phage that was filtered using a 0.45 um filter?
PFU rep 1- 121 PFU
PFU rep 2- 102 PFU
Volume stock plated- 0.1 mL
Average concentration= 5.6 x 109 PFU/mL.
Step by step
Solved in 2 steps
- Imagine you have been given a liquid culture of yeast with a starting concentration of 3.67 x 10' cells/ml and are asked to carry out the sample dilution process shown in the figure below. 100μl 100μl 100μl 100μl 100μl 0.9ml 0.9ml 0.9ml H2O H₂O 6.9ml 0.9ml H₂O H₂O H₂O Original 10-1 102 10-3 104 Culture 105 100μl 100μl 100μl Plate A Plate B Plate C a. How many colonies should have been present on Plate A in this example? - Answers must be whole numbers as partial colonies are not expected. b. Imagine you carried out the same dilution scheme shown in the figure above, but now, you do not know the concentration of the original culture. If you counted 163 colonies on Plate B, what is the concentration of cells/ml in the original culture?The total number of cells in a culture is counted using the trypan blue exclusion assay and is found to be 6.8 x 106 cells/ml. Each well in a 6 well plate requires 2 x 105 cells. How should the solution be diluted so that 1ml can be added to each well?You are given a bacterial culture which has a concentration of approximately 5.0 x 10^8 cells/mL. List a series of dilutions and platings that you could carry out in order to determine the exact concentration of the culture. Note that you must plate four plates from a minimum of two dilution tubes. The volumes plated should be in the range of 0.1 mL – 1.0 mL. Duplicate volumes may not be plated from any one dilution tube. Each plating should aim for a count between 30 and 300 CFUs. You can select any value from 30-300 for CFU and any volume from 0.1-1.0 to find out dilution scheme
- The colony forming units (CFU)/ml in sauerkraut brine are given for different types of bacteria except for leuconostoc (shaded column). To obtain the Leuconostoc counts for different days, 40 microlitre of sauerkraut brine was 10-fold diluted and the CFU was determined by plating different dilutions on agar plates. For Day 0 56 CFU were counted in 10-1 dilution, Day 1 81 CFU were counted in 10-3 dilution, Day 2 86 CFU were counted in 10-3 dilution Day 5 78 CFU were counted in 10-5 dilution Calculate the CFU/ml of leuconostoc in the brine for the 4 different days. Show your calculationA pure bacterial culture of unknown concentration was diluted to determine the concentration of viable bacteria in the original culture. Serial dilutions were performed as 2. diagrammed below. A volume of 500 µl was transferred into each tube. TSA plates were inoculated with 100 µl from the last three dilution tubes. a. If the dilution between each tube is 102, what is the volume of diluent in each of the 5 dilution tubes? Provide the volume using ml as the units. b. What is the total dilution of tube number 4? Express the total dilution using scientific notation. c. What is the concentration of viable bacteria in the original culture? Express the concentration using scientific notation and CFU/ml as the units. d. If you inoculated a TSA plate with 1.0 ml from dilution tube 4, how many colonies would you expect to form on the plate after incubation? e. If the original culture had a volume of 50ml, what was the total number of viable bacteria in the 50 ml of the original culture? 1 2 3…Please note that these counts have all been done with Trypan Blue. Therefore, all cultures have been diluted two-fold (equal volume of cell suspension + equal volume of Trypan Blue). Take this into account when determining cell number. Any dilution listed in table is an additional dilution. Live and dead cells listed below represent the total number of cells in 5 large counting squares. *1 x 105 cells/ml cells were seeded into each well of the 24 well plate(s) on day 0 to begin the growth study. Plot this value at the zero time point on your graph.1These numbers represent the total number of cells counted in 5 large squares from three separate counts.
- A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to determine the population of the culture, by transferring 25 ml of the suspension to 225 ml 0.90% NaCl. Three consecutive dilutions were further made by using I ml aliquot in 9 ml of 0.90% NaCl. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method. 田 Sampling COUNTS 1st dilution 54; 61 2nd dilution 3rd dilution 4h dilution 1st 3; 7 0; 0 0,0 2nd TNTC TNCT 242: 233 28: 37 3rd TNTC TNTC INTC 249-246 * TNTC = Too numerous to count i. Illustrate the dilution series used and label the final dilution of each dilution. ii. Determine the bacterial count (CFU/ml) every 4 hours of incubation for 12 hours. Show all computations.A serial dilution of overnight E.coli culture was performed by pipetting 1ml of a bacterial culture into a 9 ml LB medium. After this, from 10-4 and 10-5 dilution tubes 100µl were plated onto LB agar plates. Upon overnight incubation at 37°C, 200 colonies were counted in 10-4 and 22 colonies were present on 10-5 plates. How many colony-forming units were present per ml of the original culture? If the formula for CFU/ml =no. Of colonies/dilution factor*volume of culture plateTo determine the number of cells in a pure culture of Klebsiella pneumoniae, you have performed a serial dilution using three tubes of sterile saline (9.9 ml each). A sample of 0.1 ml from the culture was added to tube 1. Similarly, 0.1 ml from tube 1 was used to inoculate tube 2, and tube 3 was inoculated using 0.1 ml from tube 2. A nutrient agar plate was then inoculated using 0.1 ml from tube 3 and incubated overnight. The next day, 92 colonies were observed on the plate. How many cfu/ml were in the original culture? Using a Petroff-Hauser counting chamber, the number of cells in the same culture was estimated to be 8.5 • 109 cells/ml. How can you explain these results?
- DILUTION COLONY COUNT CFU/mL 1:106 155000 1:107 15500 1:108 1550 1:109 155 Given these values how would I fill in the rest of this serial dilution table? Also, what would be a 1:1 CFU/mL value based on this table?A phagehunter performs a full plate titer using standard techniques (100 ul of each dilution added to 250 ul of Gordonia terrae cells) of a lysate obtained from an optimum webbed plate experiment. The phagehunter counts 72 plaques on the 10-7 dilution plate. Which of the following scenarios is the best choice for the phagehunter to do next? a.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should adopt a phage from direct isolation. b.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should go back to the pick-a-plaque experiment. c.) The phagehunter has achieved a high enough titer lysate to move forward with characterization experiments. d.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should try to make more optimum webbed plates.Calculate the concentration of bacteriophage in the original culture from the following data. Be sure to include units. Show dilution factors for each test tube. Show the final dilution factor for test tube number 4. Show all your math. 0.1 ml 0.01 ml 0.001 ml 0.1 ml 1.0 ml plated 9.9 ml 9.99 ml 9.999 mi 9.9 m plaques Original Culture Test Test Test Test Tube #1 Tube #2 Tube #3 Tube #4 Dilution Factor: Concentration of Phage: