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2. How could multi-genes families complicate things in terms of using CRISPR to knock out a target gene and achieve a target
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- From your knowledge about DNA microarray, answer the following: A- How DNA microarray is created? and why it is referred to as “hybridization technology”? B- Why RT-PCR is important in the sample preparation to perform expression microarray experiment? C- Mention the name and the color of the dyes used in expression microarray? D- If the expression microarray experiment was done with a normal sample and a suspected sample, after reading the color pattern resulted from the experiment it was recorded that “gene A22” is expressed in the suspected sample. The gene A22 is clinically linked to colon cancer. Answer the following: What is the expected color of the spot on the microarray which represents this gene? What is your interpretation of the suspected sample; is it a cancer sample or not and explain why?Question:- What is genetic engineering? What organisms can it be performed on? Please give an example of at least one successful genetic engineering project. Where was CRISPR/Cas9 initially found? What was its purpose in that organism?Hi, I would like to know which program is used for the graphical presentation of the results of a meta-analysis of genome-wide linkage scans?
- 1. Why would multi-gene families complicate things in terms of being sure of which member of the family was responsible for a particular phenotype? 2. How could multi-genes families complicate things in terms of using CRISPR to knock out a target gene and achieve a target phenotype?#3) Ligase catalyzes a reaction between the 5' phosphate and the 3' hydroxyl groups at the end of DNA molecules. The enzyme calf intestinal phosphatase catalyzes the removal of the 5' phosphate from DNA molecules. What would be the consequence of treating a cloning vector, before ligation, with calf intestinal phosphatase?TOPIC: PCR and Gene Cloning Basics Question: What are 2 possible roles of CaCl2 in the transformation process?
- Is methylation a reversible process? Explain your answer. 2. What potential does epigenomics present on disease treatment?Why is genome editing by CRISPR-Cas advantageous over traditionalmethods for creating knockout or transgenic animals?Explain your answers.I get everything else in this problem other than the third option: Introduce the mutant human HD allele as a transgene into the mouse genome with transgene integration anywhere in the mouse genome. Why is the first question (left) okay with introducing mutant human HD allele and the second question (right) is not? I heard that introducing allele without using CRISPR-Cas9 is very rare and difficult. If so, how does it work in the first problem (Hungtinton's chorea)?
- 6) If you had the ability to do gene editing with ONE gene for the betterment of human kind, which one would you choose, and why? Assume you could either change an abnormal allele associated with a disease, such as the cystin gene associated with Cystic Fibrosis to its normal wild type, or add a pre-existing human allele to a genomeWhat advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications where the use of a genomic library is necessary to provide information that a cDNA library cannot.t is possible to buy a CRISPR kit that you can do in your own home. Is this a good idea? After watching the two CRISPR videos answer the following questions in your post. When is a gene editing technology safe and effective enough to be used outside of the laboratory? How do we balance risks and benefits appropriately? How far should we go in altering the future genetic landscape of free-living species, including humans? And importantly, who should decide such issues?