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- How to calculate recombination frequency between three genes? How does the map unit correlate with recombination frequency?What is a recombinant vector? How is a recombinant vector constructed? Explain how X-Gal is used in a method of identifying recombinant vectors that contain segments of chromosomal DNA.What advantages do anonymous DNA markers afford for genetic mapping as opposed to traditional allelic markers associated with visible phenotypes? What are the disadvantages of anonymous DNA markers for mapping?