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- A student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step what was done to accomplish this. The transfer of bacteria was not successful because of which step? 1. Cap of the broth culture is removed 2. The mouth of the bottle is flamed 3. The loop was flamed 4. The loop was inserted into the culture to pick up the bacteria 5. The loop was flamed 6. The loop containing the bacteria was used to introduced to spread the agar plate 7. The plate was placed in an incubator at 30 CelciusPour-Plate Method 1. Three milliliters instead of 1 mL was placed in the Petri dish. Effect:Rationale:Please make an aseptic technique and pure culture technique flowchart
- Citrate test What is the color of the medium before inoculation? Green or blueWhat is this media? What type of media is it? Which type of organism can grow on this plate? Indicate the result on the plate. What tool is used to inoculate this plate?Technique used to inoculate plate media using inoculating loop. Technique used to inoculate agar deep using inoculating needle. Pattern used to inoculate agar slant to get luxuriant culture. This pattern increases the surface area of agar that can be inoculated. Can you answer all the questions? Thankyou!