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- A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. What must be done with the bacterial sample after it is processed in the sterilizer?Use of chemical agents to destroy microbes on skin or tissues Reinoval of microbes from surfaces by scrubbing Removal or destruction of all microbes Use of chemical agents to destroy microbes on surfaces used by the public Use of physical/chemical agents to destroy microbes on non-living objects Free of contamination by pathogens Antisepsis (antiseptic) Aseptic Degerming Sanitization Sterilization DisinfectionFor sterilization to occur correctly it must include; heat and pressure: true False
- Should you sample an area for microbial growth before or after sanitization? Eplain why.to minimize health hazard, it is necessary to sterilize : Associated microbiological materials Soiled dressings Other contaminated items All choices are correctThese are examples of the 5 rules of asepsis, except: 1. Any item used for patient care or that is visibly soiled must be considered "dirty" and "contaminated" 2. If the item has been cleaned with a detergent disinfectant, it is both clean and decontaminated 3. Sterile items are devices processed by steam, dry heat, ethylene oxide or other sterilization method to kill most organisms 4. Dirty, clean and sterile items must be separated at all times
- Which one of the following microorganisms would you use as a quality control check to ensure that your sterile technique method is properly working? S. aureus, a gram-positive bacterium E. coli, a gram-negative bacterium B. cereus, an endospore-forming bacterium M. leprae, an acid-fast bacterium T. pallidum, a flagellated, disease causing bacteriumPlastic pipet tips for micropipettes are commonly supplied in packs of 100’s. Once the pack has been opened, contamination is assumed. How can sterility of the pipet tips be ensured prior to use in microbiological procedures? METHOD:RATIONALE:Differentiate between a pure culture, subculture, mixed culture,and contaminated culture. Define contaminant.
- You are rotating sterile supplies based on the first in, first out (FIFO) principle. What would you look at? O Sterilization method Expiration date Manufacturing date Packaging materialAs an aspiring Nurse, the need for the culture of a new pathogenic microorganism in the laboratory has been allocated to you. Applying all the guidelines involved in the successful isolation and cultivation of microbes, discuss the culturing system that is most appropriate for this activity and state your reason(s) for this choiceTechnique used to inoculate plate media using inoculating loop. Technique used to inoculate agar deep using inoculating needle. Pattern used to inoculate agar slant to get luxuriant culture. This pattern increases the surface area of agar that can be inoculated. Can you answer all the questions? Thankyou!