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Give atleast 3 differences between UV and PDA detector and explain
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- Which of the ff. best describes the configuration of a traditionalcolorimeter? a.) light source --> wavelength selector --> sample holder --> wavelength detector --> photon detectorb.) light source --> sample holder -> wavelength detector -> photon detectorc.) light source --> wavelength selector --> sample holder --> photon detectord.) light source --> wavelength selector --> sample holder --> wavelength detectorDescribe the mechanism of SERS( Surface Enhanced Raman Spectroscopy).1. What are the three properties of laser light that are different than normallight? 2. How is distention achieved in minimally invasive procedures, and why isit necessary?
- The following is an UV-Vis schematic diagram. Based on this diagram, answer the following questions: D2 Lamp (B) Detector Light Source (A) Sample What are the components labelled as (A) and (B)? Briefly explain the functions of them.What is the main principle that governs aseptic technique? Explain in minimum of 4-5 sentences.If we used the 365nm setting on the UV light box would this have caused more or less UV light damage, explain why?
- The significance of using immersion oil when using 1OOX objective lens is The significance of using immersion oll when uskng 100X objective lens is: It will increase the contrast by having same refractive index as the lens, loss of light by refraction, reflection and diffraction is minimized. the specimens need not be killed, fixed and stained more light is captured for better illumination, allowing the specimen to fluoresce i. il. iii. iv. A light microscope should have the following features contrast Resolution magnification fluorescence ii. iv.1. Describe expected EFM reading with a patient with preeclampsia include rationale.Explain the usage of photoactivated localization microscopy technique.
- How is the UV spectrometer work? What is absorbance and how is it determined?What is the principle behind a UV-Vis spectrophotometer, and what are its key components, and how does it work?Answer this ff question: 1. Explain the importance of system suitability on HPLC method of analysis. 2.In UV and visible spectrophotometry, the specimen is generally dissolved in a solvent, and determinations are made at room temperature using a path length of 1 cm. Give the most common solvents suitable for UV or visible spectrophotometry.