Generalized transduction by random incorporation of bacterial DNA into phage heads Donor bacterium Phages carrying donor genes Transduced bacterium Recipient bacterium FIGURE 5-29 A newly forming phage may pick up DNA from its host cel's chromosome (top) and then inject it into a new cel(bottam right). The irjected DNA may insert into the new host's chromosome by recombination (bottom left). In reality orly a very smal minority of phage progeny (1 in 10,000) carry donor genes.

Generalized transduction by random incorporation of bacterial DNA into phage heads Donor bacterium Phages carrying donor genes Transduced bacterium Recipient bacterium FIGURE 5-29 A newly forming phage may pick up DNA from its host cel's chromosome (top) and then inject it into a new cel(bottam right). The irjected DNA may insert into the new host's chromosome by recombination (bottom left). In reality orly a very smal minority of phage progeny (1 in 10,000) carry donor genes.

Biology: The Unity and Diversity of Life (MindTap Course List)

14th Edition

ISBN:9781305073951

Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Chapter8: Dna Structure And Function

Section: Chapter Questions

Problem 1DAA: HersheyChase Experiments The graph shown in FIGURE 8.5 is reproduced from an original 1952...

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As described in Figure, host DNA is hydrolyzed into smallpieces, which are occasionally assembled with phage proteins, creatinga phage with bacterial chromosomal DNA. If the breakage ofthe chromosomal DNA is not random (i.e., it is more likely tobreak at certain spots as opposed to other spots), how might nonrandombreakage affect cotransduction frequency?
An ade+ arg+ cys+ his+ leu+ pro+ bacterial strain is knownto be lysogenic for a newly discovered phage, but the siteof the prophage is not known. The bacterial map isleucysarghisadeproThe lysogenic strain is used as a source of the phage, andthe phages are added to a bacterial strain of genotypeade- arg- cys- his- leu- pro-. After a short incubation,samples of these bacteria are plated on six differentmedia, with the supplementations indicated in thefollowing table. The table also shows whether colonieswere observed on the various media.PresenceMedium Ade Arg Cys His Leu Pro of colonies1 - + + + + + N2 + - + + + + N3 + + - + + + C4 + + + - + + N5 + + + + - + C6 + + + + + - NNutrient supplementation in medium(In this table, a plus sign indicates the presence of anutrient supplement, a minus sign indicates that asupplement is not present, N indicates no colonies, and Cindicates colonies present.)a. What genetic process is at work here?b. What is the approximate locus of the prophage?
If you extract the DNA of the coliphage φ X174, you willfind that its composition is 25 percent A, 33 percent T,24 percent G, and 18 percent C. Does this compositionmake sense in regard to Chargaff’s rules? How wouldyou interpret this result? How might such a phage replicate its DNA
A researcher identified a mutation in PR of phage λ that causes itstranscription rate to be increased 10-fold. Do you think this mutationwould favor the lytic or lysogenic cycle? Explain your answer.
About the technique of phage display: MOLECULAR BIOLOGY_advanced The Escherichia coli cell infected by the phage codifies for the optimized ligand when the phage DNA integrates in the DNA of the bacteria. Phages are selected if they express on their surface the optimized ligand. One selects Escherichia coli cells that are resistant to the phage infection. More than one optimized ligand can be selected during the panning procedure. The ligand to be selected on the surface of the phage is non-covalently linked to one of the surface proteins.
Which structure is result of phage genome site on bacterial chromosome?
In the lysogenic cycle of bacteriophages, which of the fol-lowing is true?(a) Lysogeny is the term applied to a temperate phage thatdoes not replicate itself independently and does not lysethe bacterial host cell.(b) Phage DNA is incorporated into the host bacterium’sDNA, at which time it is called a prophage.(c) Temperate phages can be replicated either as a pro-phage along with bacterial chromosomal replicationor can suddenly revert to the lytic cycle by replicatingthemselves and assembling into new phages.(d) All the above are true.(e) a and c only.
draw structure of plasmid
When bacteriophage P1 causes E. coli to lyse, the resulting materialis called a P1 lysate. What type of genetic material would befound in most of the P1 phages in the lysate? What kind of geneticmaterial is occasionally found within a P1 phage?
Which step(s) occur in the lysogenic replication cycle of temperate phage but does not occur in the lytic replication cycle? O adsorption O biosynthesis O assembly O release O formation of prophage and lysogen
Three pairs of bacterial cells with the given genotypes undergo conjugation. Place match the genotype of each cell after conjugation to its initial genotype. F+ x F Hfr F- F' F- Answer Bank F F F+ Hfr What is the role of the F-factor in conjugation? It contains genes necessary for replication of the donor's F plasmid. can occur. It allows auxotrophic bacterial cells to survive on minimal medium so that conjugation It contains genes that force recombination between the donor and recipient chromosomes. It contains genes necessary for the formation of the pilus. O It degrades the chromosome of the recipient cell after conjugation.
Experimentally, when an E. coli bacterium already has a λ prophageintegrated into its chromosome, another λ phage cannot usually infectthe cell and establish the lysogenic or lytic cycle. Based on yourunderstanding of the genetic regulation of the phage λ reproductivecycles, why do you think the other phage would be unsuccessful?