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- 5 1 point What are the units used to measure the concentration of phage in a lysate? plaques/uL Oplaques/mL plaque forming units/mL plaque forming units/uL Previous22:23 1O 000 · 11:24 A9 OB1 r ll l 52% . +964 782 734 3923 2m541139927815107... Patient Encounter Part 3 The pretreatment workup is summarized below. Pathology: 47-year-old female with new diagnosis of infiltrating intraductal adenocarcinoma involving the left breast and regional node. Further tests on tumor samples indicated ER (8%), PR (negative), HER2 (negative), Ki-67 (72%), and grade (poorly differentiated). Intrinsic subtype (luminal B, HER2-negative). Radiology: FDG-PET/CT indicated a 5.3 x 2.5 cm mass in the left breast which appeared to extend to the epidermis of the skin; one node in the left axilla was also involved with tumor. No other evidence of distant disease was visualized. Laboratory: CBC, liver, and kidney function tests WNL, alkaline phosphatase and calcium are normal also. Stage: IB (T, N, M,) List the most important prognostic factors in this patient with newly diagnosed breast cancer. Assess the patient's level of risk for relapse. 50 SECTION 16 | ONCOLOGIC…Phenotype: A2B 1. Give the possible genotype/s of A:B 2. If you were to perform the blood typing of this individual, what are the expected results given the following reagents Phenotype Forward typing (reagents) Reverse typing (reagents) Anti-A Anti-B Anti-H A cells B cells Al Write "+" for presence of agglutination and "-" for absence of agglutination 3. Explain the results of the blood typing, why would there be a "+" or a "" reaction during the testing. Relate your answer to the presence/absence of antigens and/or antibodies
- B2. During a mass spectrometry experiment, the spectrum of vascular endothelial growth factor A (VEGF-A) was acquired. The absolute error of VEGF-A, having a mass of 1024.5210 Da was 0.06 Da. Calculate the relative error (ppm). (10 marks)A physician is typing the blood specimen belonging to an eighteen-year-old female to receive emergency blood transfusions due to an unfortunate accident. The following are the results: Anti-A Anti-B Anti-D A1 Cells B Cells 4+ - - - 4+ Due to the initial negative Anti-D result, the physician decided to perform an indirect antiglobulin test. The following are the results: Anti-D Control Positive Neg Answer the following questions: Which conditions warrant the initial Anti-D result? Which specific test was done to confirm an initial Rh negative result? State the principle of the test, its clinical applications, and procedure.Southern Blotting & Detection of sickle cell disease: - Please label the figure and discuss each lane, and draw a Punnett square. - Finally, discuss the results at length.
- 1) Explain the principle of the electrical impedance cell counting. 2) State 3 Haematology analyzer and their manufacturers. 3) CV for automated instrument is +/- 1% to +/- 2% compared to manual count with CV +/- 10%. Can you explain their differences?5) The immunoblot shows probing of samples taken from a time course after treatment with the proteasome inhibitor MG132. Recall that the proteasome is responsible for selectively degrading only those proteins that have been tagged with ubiquitin. The left indicates the antibody that is used to detect protein for each panel. P53 P27 AGR2 GAPDH MG132 (h) 0 0.5 1 2 4 8 12 24 Image from Wang et al., (2019) Oncogene 38:3458-3474. a) What effect does MG132 have on each protein? What does this tell you about each of these proteins? ● P53 P27 AGR2 GAPDH b) How do we know that the differences we observe in the proteins is not due to un- equal loading of samples into each lane of the gel?B. The concentration profile of drug diffusion in a tumor spheroid is given by: Qr3 ди + C = r². 3D ar a. Derive an equation for u in terms of r b. Find u, when drug dissolves only to 10% from the surface of the spheroid (assume the radius of the spheroid as b; the concentration of drug at the surface as up). c. Plot the tumor spheroid concentration profile.
- Key: √ : Agglutination X: No agglutination Donors Recipients Type A+ Type A- Type B+ Type B- Type AB+ Type AB- Type O+ Type O- Type A+ Type A- Type B+ Type B- Type AB+ Type AB- Type O+ Type O-A physician is typing the blood specimen belonging to an eighteen-year-old female to receive emergency blood transfusions due to an unfortunate accident. The following are the results: Anti-A Anti-B Anti-D A1 Cells B Cells 4+ - - - 4+ Due to the initial negative Anti-D result, the medical technologist decided to perform an indirect antiglobulin test. The following are the results: Anti-D Control Positive Neg Using the IAT results, should the patient be considered Rh positive or Rh negative? Justify.3..What ligand on transferrin is responsible for the red color of the iron loaded transferrin. please explain using pea values.