Distance Passed Partition Pigment Spot Color (mm) Coefficient, R Chlorophyll b Yellow green 22 22/54 = 0.407 25/54 = 0.462 Chlorophyll a Blue green 25 30 Bright yellow Yellow orange Xanthophyll 30/54 = 0.555 Carotene 52 52/54 = 0.962 Distance passed by the solvent system (solvent front) 54mm a. Which pigment travelled the longest distance? What does this tell you about that pigment? b. Which pigment travelled the shortest distance? What does that telly about that pigment? lyou
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- PART III - Table 3: Absorption Spectrum Data for Spinach Chloroplast Extract Absorbance Wavelength 620 nm 0.147 640 nm 0.058 660 nm 680 nm 700 nm 720 nm Wavelength 380 nm 400 nm 420 nm 440 nm 460 nm 480 nm Absorbance 0.759 0.923 Questions: 1.095 1.075 0.833 10.520 Wavelength 500 nm 520 nm 540 nm 560 nm 580 nm 600 nm 0.054 0.066 0.094 123 Graph: Plot the absorbance data on the graph below. Remember TAILS. Absorbance · 163 ·19 4 •514 · 166 1012 ·003 1. Your graph should have two "peaks." At the highest of the two, what wavelength was most strongly absorbed by the chloroplast pigments? What wavelength corresponds the top of the second peak? What wavelength was least absorbed by the chloroplast pigments? 2. Based on your observations, which wavelength (color) of light might be expected to generate the lowest rate of photosynthesis? 91The amount of phytomenadione contained in a O.2 mL ampoule of 10 * .mg/mL solutionAn ion exchange resin prefers calcium over magnesium, indicated by Xcalcium = 0.6 and Ymagnesium = 0.3. The same resin prefers magnesium over sodium, indicated by Xmagnesium = 0.6, Ysodium= 0.2, What is the separation factor for the resin for calcium over sodium?
- Why is 80% alcohol the recommended solvent For the preparation of plant extract?Test-tube Beaker Funnel Water plants In this setup, the rate of the reaction can be increased by o decreasing the light intensity O adding sodium bicarbonate only O adding more water to the beaker O increasing the light intensity only O adding sodium bicarbonate and increasing the light intensity Clear my choiceThe authors of this paper also tested another minimal media (Media C) which is exactly the same as Media B except that the pH is 7.4 instead of 7.1. Which media components would be different in Media C? For each component that is different, state if will be larger or smaller than the amount in Media B.
- Media - Possible Results Туре of Medium Original Color Any Chemicals Added to Visualize Reaction? Negative Result Positive Result Phenol Red Glucose Phenol Red Lactose Sulfur: Sulfur: SIM Indole: Indole: Motility: Motility: MR VP Citrate Slant Nitrate Broth 6.5% NaCi TSB Bile Esculin For the Fluid Thioglycollate Medium, draw the five possible outcomes in the sections below and label accordingly. (obligate aerobe, obligate anaerobe, facultative anaerobe, aerotolerant anaerobe, microaerophile) Fluid Thioglycollate Medium Novobiocin Sensitivity Test : Use the Difco Manual for the following and describe the possible outcomes: EMB: Mannitol Salt Agar:Identify the zwitterion below and at what pH it can be foundWhich color pigment was most attracted to the mobile phase? Solvent Blue O Red O Yellow After Ten Minutes O None are attracted to developing solution. E OMIN @
- 57. A sample of white petrolatum contains 10 mg of tocopherol per kilogram as a pre- servative. Express the amount of tocoph- erol as a ratio strength. 57. 1:100,000 w/w tocopherolExplain why you can use OD675 (optical density at a wavelength of 675nm) as a proxy for cell density of your algal cultures.Why efficascent oil is green liquid?