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- 1.. What is the useful operating volume range of a Gilson P-10 pipette? Where did you find this information? 2.. Which micropipette should be used to dispense 4.50 x10-4 L of water? Explain your choice. 3. What should 2.50 x 10-4 L of water weigh at 27 °C? (Show calculations and include proper units with your answer) 4. Determine the temperature in the room where a gravimetric pipette calibration is being conducted. A 100 μl volume yielded a weight of 99.46 mg. Show your work for full credit.A 10-L stirred tank is used to cultivate mouse myeloma cells. Dry air is sparged from the bottom at 1.0 L/min to supply oxygen to the system. The gas composition at the outler is 16% oxygen, 3.5% CO2, 6.5% water vapor, and balanced nitrogen. The ambient pressure and temperature are 1 atm and 37 °C, respectively. The dissolved oxygen in the reactor is controlled at 20% of saturation with ambient air. What is the respiratory quotinent (RQ) (i.e., mole CO2 produced/mol O2 consumed)? Assume that the mole fraction of CO2 in the ambient air is negligible. The universal gas constant R = 0.082 L*atm/mol*KA 10-L stirred tank is used to cultivate mouse myeloma cells. Dry air is sparged from the bottom at 1.0 L/min to supply oxygen to the system. The gas composition at the outler is 16% oxygen, 3.5% CO2, 6.5% water vapor, and balanced nitrogen. The ambient pressure and temperature are 1 atm and 37 °C, respectively. The dissolved oxygen in the reactor is controlled at 20% of saturation with ambient air. a. Assuming the reactor is at a steady-state, what is the volumetric oxygen transfer coefficient (kLa)? b. The specific OUR of myeloma cells is 5*10-11 mmol/cell*h. What is the cell concentration? c. What is the respiratory quotinent (RQ) (i.e., mole CO2 produced/mol O2 consumed)? Assume that the mole fraction of CO2 in the ambient air is negligible. The universal gas constant R = 0.082 L*atm/mol*K
- 4. A 1.0 M solution of copper(II) sulfate is electrolyzed using platinum electrodes. If 1.50 g of copper metal is deposited on the cathode, how many moles of oxygen gas were produced at the anode during the same time period?3.1. List six (6) factors to be considered when designing a centrifugation protocol in a biomedical laboratory 3.2. A biomedical laboratory technician decides to centrifuge a biological sample so that it experiences a relative centrifugal force of 100 000 x g. At what rpm must the technician set the centrifuge, assuming an average r value of 4? 3.3. You are replicating an experimental technique from a journal article relating to the isolation of lysosomal fractions from plant material. The authors stated that they centrifuged their sample in a sucrose density gradient at 6000 x g for 20 minutes. You can only set your centrifuge in rpm. If the radius from axis of rotation is 10 cm at the top and 4 cm at the bottom, what settings would you use on your centrifuge (Answer in rpm)?Explain the differences, if any, between adsorption and gas solid chromatography
- 1-solid, 2-liquid, 3-gas, 4-supercritical fluid, point O-triple point, C-critical point -78.5 °C (The phase of dry ice changes from solid to gas at -78.5 °C) 3. Describe what one would see at pressures and temperatures above 5 atm and 1000°C. Answer:Which of the following statements about AAS and AES is FALSE a)Sample used in AAS are usually in the form of aqueous solution b)In flame AAS, the flame is used for atomization as well as excitation purpose c)The graphite furnace is an example of non-flame atomizer d)In flame AES, no light source is required. What is the advantages of Axial torch configuration over Radial configuration in Inductive Couple Plasma-Optical Emission Spectrometer (ICP-OES) a)Increased radiation intensity – a longer path length b)Excellent tolerance to dissolve solids c)Can analyze S, P, and Halogens d)Excellent torch lifetimesLab students placed ten spinach leaf disks in each of three test tubes containing a baking soda solution and a control tube without baking soda. The volume of liquid in each tube and the temperature of the solutions were held constant. All tubes were placed an equal distance from a light source as indicated in the accompanying table. After 10 minutes, the amount of oxygen present in each sample was measured. Test Tube Grams of Baking Soda (CO2 source) Wattage of Light Bulb 1 0.5 25 2 0.5 50 3 0.5 75 4 0 75 Which tube is expected to contain the greatest quantity of oxygen? Tube 1 Tube 2 Tube 3 Tube 4
- What volume of a 40% v/v solution must be used to produce 500mL of 5% v/v solution?Lab students placed ten spinach leaf disks in each of three test tubes containing a baking soda solution and a control tube without baking soda. The volume of liquid in each tube and the temperature of the solutions were held constant. All tubes were placed an equal distance from the light source indicated in the accompanying table. After 10 minutes, the amount of oxygen present in each sample was measured. Test Tube Grams of Baking Soda (CO2 source) Wattage of Light Bulb 1 0.5 25 2 0.5 50 3 0.5 75 4 0 75 Which tube is expected to produce the least amount of oxygen? a) Tube 1 b) Tube 2 c) Tube 3 d) Tube 4Explain about the EPO experiments.