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- Discuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two tips on its proper use.
- Explain how calcium ions are confirmed present in a solid salt by using flame test.
- Discuss a method to confirm the presence of a) phenol and b)
ketone in a test compound. - How are proteins confirmed present in a sample? Explain the laboratory process.
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- Draw/illustrate the chemical reaction involved in Lugol’s test and explain the purpose of this qualitative test?Answer the following questions (not more than 5 sentences/question). Discuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two tips on its proper use. Explain how calcium ions are confirmed present in a solid salt by using flame test. Discuss a method to confirm the presence of a) phenol and b) ketone in a test compound. How are proteins confirmed present in a sample? Explain the laboratory process.The table below summarizes the results for Millon's test. Provide the correct remarks from the results if Negative for Millon's Test or Positive for Millon's Test and if Contains phenolic hydroxy group or Does not contains phenolic hydroxy group.
- Using an official compendium, enumerate the different general tests required for the for the raw materials, N-acetyl-cysteine, include the method of assay used. Describe the types of glass and plastic containers used for pharmaceutical products Discuss briefly the different tests under general chapter <661> - Containers, of USPProvide the Experimental procedure of the following: Example: Ninhydrin Test -The samples needed in this test were 1% casein solution, albumin and gelatin and the reagent used was ninhydrin (Triketohydrindenehydrate or Triketohydrin hydrate). Test tube, test tube stand, pippete, water bath, and spectrophotometer were the materials and equipment used. Ninhydrin reagent in 1-2 drops was added to 0.5 ml of the sample with care. It was mixed and heated to boiling for 1-2 minutes. Then, it was allowed to cool to record the observation. Test that needs Experimental procedure: 1. Heat denaturation Samples: Albumin, gelatin 2. Isoelectric Precipitation Samples: Casein 3. Concentrated Mineral Acids Samples: Albumin 4. Organic Solvents Samples: AlbuminDifferentiate Wintrobe from Westergren regarding accuracy of the test in a tabulated order.
- Write down the materials, reagents, Principle, procedure and results for Methamine silver technique.Please explain the theoretical background (positive color reactions and what is being detected) involved in the different qualitative test for proteins that has been isolated intact or hydrolyzed. Please directly answer the theoretical background (positive color reactions and what is being detected) of each test GENERAL: BIURET - NINHYDRIN SPECIFIC XANTHOPROTEIC TEST - MILLON'S TEST - HOPKINS-COLE TEST - SAKAGUCHI TEST - LEAD ACETATE TEST - NITROPRUSSIDE TEST - FOLLIN'S REACTION - SULLIVAN'S TEST - PAULY REACTION - LIEBERMAN'S TEST -Provide the Principles and detailed procedures of the following tests for the "Color Reaction of Proteins". 1. Hopkins-Cole Test 2. Sulfur Test 3. Heller's Test
- Give the chemical principles involved in a positive Biuret test, Millon’s Test and Sakaguchi Test. What is the chemical basis of the Ninhydrin Test, Hopkins – Cole test and Sulfur reaction test? What chemical structure should be present in an amino acid or protein to give a positive Xanthoproteic test?How are clinical chemistry test results used to confirm or rule out a presumptive diagnosis? Why is this important and give references.What is the most important component that will be tested using the reagent test strips?