Determine how the following will affect the calculated ppm CaCO3 in the EDTA Experiment. Determine if INCREASE, DECREASE, or NO EFFECT. a. The sample container was contaminated with an acid b. A small amount of iron(III) nitrate solution was accidentally added to the sample c. Instead of using NH3/NH4+ buffer, Na2CO3/NaHCO3 buffer at pH 10 was used
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Determine how the following will affect the calculated ppm CaCO3 in the EDTA Experiment. Determine if INCREASE, DECREASE, or NO EFFECT.
a. The sample container was contaminated with an acid
b. A small amount of iron(III) nitrate solution was accidentally added to the sample
c. Instead of using NH3/NH4+ buffer, Na2CO3/NaHCO3 buffer at pH 10 was used
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- Out of the potential unknowns in this experiment, which one would be the best choice to create a buffer with a pH of 7.0? Note: Copying and/or posting this question without the express written permission from Dr. Burke and the Department of Chemistry & Biochemistry at the University of Delaware is a violation of intellectual property copyright law. Out of the potential unknowns in this experiment, which one would be the best choice to create a buffer with a pH of 7.0? Formic acid Sodium bisulfate Phenol Hypobromous acid Boric acid Sodium bicarbonate Potassium hydrogen phthalate Sodium bisulfite Disodium hydrogen citrate Ascorbic acidThe substance to be analyzed - is white, highly soluble in water salt. Addition of diluted sulfuric acid, or hydrochloric acid, or NaOH, or barium chloride, or silver nitrate doesn't produce any precipitates. Interaction with Nessler's reagent leads to formation of reddish-brown precipitate. Addition of antipyrin in acid medium changes color to red. What kind of substance is this? NH,NO; KNO, NaCl NH,CH;COOAn auxiliary competing agent is used in EDTA titrations to slow down the titration. keep some interfering component of the analyte mixture from complexing with the EDTA. speed up the titration. keep the metal ion from precipitating out of the solution.
- 2. Suggest a maximum buffering capacity for each buffer. Look for their corresponding pka values first, you can find these in your textbooks or online sources (eg. Campbell's Biochemistry, 9th ed). Buffering Сараcity Maximum Buffering Сараcity Buffer Components pK value Ammonium/Ammonia buffer Lactic acid/Lactate buffer Phosphoric acid/hydrogen PO4 buffer Benzoic acid/Benzoate buffer Follow-up question: Which of these buffers would you use if you need to carry out a PCR reaction using Taq polymerase which has an optimum pH at 7.2? Suggest only one buffer. Answer: Why?8. Calculate the volume of a 0.10 mol dm³ sodium ethanoate solution needed to be added to 1 dm³ of 0.10 mol dm³ ethanoic acid to produce a buffer solution with pH = 4.0. [pka for CH3COOH = 4.74]The substance to be analyzed – is white, highly soluble in water salt. Addition of diluted sulfuric acid, or hydrochloric acid, or NaOH, or barium chloride, or silver nitrate doesn't produce any precipitates. Interaction with Nessler's reagent leads to formation of reddish-brown precipitate. Addition of antipyrin in acid medium changes color to red. What kind of substance is this? NH,NO3 KNO3 NaCl NH,CH;COO
- Following the monograph procedure, determine the weight in grams of sodium carbonate (MW-106 g/mol) used to standardize a 0.987 N sulfuric acid solution. 1. Consider that the burette was completely filled to the 0 mL mark before titrating. What is the volume of titrant consumed based from the image below? A. 22.9 mL B. 21.2 mL C. 21.3 mL D. 22.7 mL 2. What is the unknown weight (grams) in the problem? Your Answer:4. To perform a certain activity assay it is necessary to have 0.25 mL of an enzyme at a concentration of 0.2 mgml¹ in the presence of 0.4 mM substrate and 200 mM NaCl in a suitable buffer. The stock solutions of enzyme, substrate and NaCl are 4 mgmL-¹, 40 mM and 1 M all in the same type of buffer. What volumes of the stock solutions and buffer are required for each assay?1.) Given the Ksp for Ag2CrO4 is equal to 1.20 x 10-12, calculate the percent error in your experimentally determined value for average Ksp (this will be an extremely large percentage - think about why). The Experimental Value: 9.99 x 10-11 2.) Using the slightly soluble salt Mg3(PO4)2, write the solubility product constant expression and the solubility equilibrium equation (dissolution equation).
- Student A accidentally added 2 M KOH during the first step of their extraction, instead of 1 M K2HPO4. Is this a problem? No. The order of addition of the basic solutions does not matter. No. The acid and weak acid can be separated later when adding HCI to the mixture. No. KOH will just deprotonate the weak acid, leaving the acidic compound in the organic layer. Yes. KOH will deprotonate both the acid and weak acid, making them difficult to separate later.17. This type of analysis implies that the constituent determined was present in very, very low concentration. a. Macro analysis b. Semi-macro analysis c. Micro analysis 18. Which of the following does not happen during digestion? The crystals dissolve and re-precipitate under equilibrium conditions. b. Weakly bound water are lost from the precipitates. c. Filterability improves d. None of the choicesItem 15 15 of 27 Constants | Periodic Table MISSED THIS? Read Section 18.2 (Pages 788 - 799); Watch KCV 18.2B, IWE 18.2. Part A A buffer with a pH of 10.95 contains CH3NH2 and CH3NH3C1 in water. What can you conclude about the relative concentrations of CH3NH2 and CH3NH3CI in this buffer? For CH3NH2, pK, = 3.36. O CH3NH2 CH3NH3C1 > O CH3NH2 CH3NH3C1 O Nothing can be concluded about the relative concentrations of CH NH, and CH,NH;Cl.