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Alpe
Describe the tests used to identify Strep, and use a dichotomous key to successfully identify an unknown (what to look for in each)
a. Hemolysis
b. Optochin sensitivity
c. Bacitracin sensitivity
d. Growth in high salt environment
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- An elderly man with influenza acquires a case of pneumonia. Gram-positive cocci isolated from his sputum give beta-hemolysis on blood agar. The infection is very difficult to treat. Later, it is shown that the man shared the room with a patient with bone infection. Isolates from both infections were the same. a. Describe 2 biochemical tests that can be used for the definitive diagnosis. b. Describe 2 virulence factors of this pathogen and implicate them in diseases.Kligler’s iron agar and SIM are multiple test media.a. What physiological characteristic is detected by both of these media? b. What component of both media allows you to detect the preceding characteristic? c. Both media are stabbed but for different reasons. Explain.On the four compounds testes for antibacterial activity using agar well diffusion assay, a. Which compound exhibit antibacterial activity? b. Which of the compounds axhibit bacteriostatic effect ____ and ____.
- What staining is used for this microspecimen? a. Methilen blu b. Toluidin blu c. Eosinophilin d. Eosin e. Hematoxilin O f. PAS-ABEx. 17: Chemotherapeutic Agents Using the Antimicrobial Susceptibility Table on pg 62 in the lab manual, determine whether not the bacteria plated in this Kirby-Bauer test are resistant (R), intermediate (I), or susceptible (S). The bacterial species tested was Escherichia coli (Gram negative). Ciprofloxacin Diameter = 20 Streptomycin Diameter 30 Carbenicillin Diameter 17 + Erythromycin Diameter = 23 Novobiocin Diameter = 0 bacterial growth = disk with antibiotic (see labels) = no growthWhich one of the following methods of antimicrobial susceptibility testing combines some of the properties of two other methods and involves adding a plastic strip to the agar? Select one: a. Agar macrodilution method b. Gradient diffusion method c. Broth microdilution method d. Disk diffusion method
- Which one of the following methods of antimicrobial susceptibility testing requires a dilution series of the antibiotic and can provide a minimum inhibitory concentration? Select one: a. Disk diffusion method b. Broth microdilution method c. Agar macrodilution method d. Gradient diffusion methodNo zone of inhibition was seen on the Blood Agar Plate with the Optochin Test. How should the microorganism be characterized with Optochin? O This is nothing significant. The microorganism is resistant to Optochin. O The microorganism is sensitive to Optochin. O There is no change.For each of the following aspects, compare and contrast the EnteroPluri-Test System to the API® 20E System for Enterobacteriaceae identification. a. time requirement b. specimen preparation c. tests utilized d. anaerobic conditions e. interpretation of results
- Antisera used in blood typing contain: A. both agglutinins and agglutinogens B. agglutinins C. agglutinogensThe Kirby -Bauer antimicrobial sensitivity testing method relies on: A. The inhibition of one antimicrobial by another B. The diffusion of antimicrobials in paper discs C. Serial dilutions of antimicrobials in test tubes D. A gradient of an antimicrobial on a paper stripExplain why the bacitracin and novobiocin tests have different cutoff measurements for sensitivity/resistance.