D.3 Loading Samples and Running the Gel Use special gel loading tips or a micro-syringe to load the complete sample in a narrow well. Take care not to poke the well bottom with the tip as this will create a distorted band. Never overfill wells. This could lead to poor data if samples spill into adjacent wells, and poorly resolved bands. Load 20-40 μg total protein per mini-gel well. Q10. If you have a lysate with protein concentration of 1500 ug/mL, how much sample you need to load according to the step indicated in D.3? Q8. For leptin, what gel percentage are you going to prepare? Q.11. Give at least two common loading control used in Western Blotting?

D.3 Loading Samples and Running the Gel Use special gel loading tips or a micro-syringe to load the complete sample in a narrow well. Take care not to poke the well bottom with the tip as this will create a distorted band. Never overfill wells. This could lead to poor data if samples spill into adjacent wells, and poorly resolved bands. Load 20-40 μg total protein per mini-gel well. Q10. If you have a lysate with protein concentration of 1500 ug/mL, how much sample you need to load according to the step indicated in D.3? Q8. For leptin, what gel percentage are you going to prepare? Q.11. Give at least two common loading control used in Western Blotting?

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter14: Formula Method

Section: Chapter Questions

Problem 40SST

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