Answered: Consider the retention time of a…

Consider the retention time of a pesticide is.13 8.5 min and the peak width at the base = 1.7 min, length of the column 30 cm, then plate height equals

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter26: An Introduction To Chromatographic Separations

Section: Chapter Questions

Problem 26.14QAP

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In analytical chemistry, when and why do we do representative sampling?
Retention time is the time taken for the mobile phase to reach the detector. True False
Give examples of sample analyte in a matrix.
A solute has a retention time of 5.4 min with a width at half height of 0.41 min on a column 12.2 m long. Find the number of plates. Please round-off your answer to a whole number (no decimal place)
Pick the HETP (height equivalent to a theoretical plate) listed below that represents the most efficient column. O1 mm/plate O 1 micrometer/plate 1 cm/plate 1 m/plate
The internal standard method compensates for errors that affect both the analyte and the reference. True False
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A solute with a retention time of 407 seconds has a width at the base of 13.0 seconds on a column 12.2 meters long. Find the number of plates and plate height. The half-width of the same peak is 7.6 seconds. Find the plate height.
Suppose that the sample size was 3.0 mg, the column dimensions were 1.00 m length 3 4.0 mm diameter, and the flow rate was 7.0 mL/min. What size column and what flow rate should be used to obtain the same quality of separation of 27.0 mg of sample?
From the data calculate: a) retention factor of each solute b) selectivity factor c) how many plates are needed to provide a resolution of 1.5? d)how long a column is needed if the plate height of the packing is 1.5x10^-3cm? e)if the flow rate is 6.75 cm/min how long will it take to elite the two species?
One method for the quantitative determination of the concentration of constituents in a sample analyzed by gas chromatography is area normalization. Here, complete elution of all the sample constituents is necessary. The area of each peak is then measured and corrected for differences in detector response to the different eluates. This correction involves dividing the area by an empirically determined correction factor. The concentration of the analyte is found from the ratio of its corrected area to the total corrected area of all peaks. For a chromatogram containing three peaks, the relative areas were found to be 16.4, 45.2 and 30.2, in order of increasing retention time. Calculate the percentage of each compound if the relative detector responses were 0.60, 0.78 and 0.88, respectively.
One method for quantitative determination of the concentration of constituents in a sample analyzed by gas chromatography is the area normalization method. In this method, complete elution of all of the sample constituents is necessary. The area of each peak is then measured and corrected for differences in detector response to the different eluates. This correction is accomplished by dividing the area by an empirically determined correction factor. The concentration of the analyte is found from the ratio of its corrected area to the total corrected area of all peaks. For a chromatogram containing three peaks, the relative areas were found to be 16.4, 45.2, and 30.2 in the order of increasing retention time. Calculate the percentage of each compound if the relative detector responses were 0.60, 0.78, and 0.88, respectively