Complete this Master Mix table for 8 DNA samples, a positive control, negative control, andan extra reaction for pipetting error. Show your work.Master Mix Conc. OfTotal μLµL/Rxn(total pertube)FinalNumber ofStockConc.Reactionsneeded forsolutionmaster mixPCR buffer50X1XWaterDNTP mix100 mM200 µMMgCl2Forward24 mM2.5 mM2 μΜ0.1 μΜprimerReverse2 μΜ0.1 μΜprimerTaqpolymeraseDNA5 U/uL0.03 U/ µL1 μL60 μLTotalvolume ofthe entirereaction(µL)

Volume per tube (uL) number of reactions volume in master mix (uL) PCR buffer 1.2 11 13.2 dNTP…

Complete this Master Mix table for 8 DNA samples, a positive control, negative control, and an extra reaction for pipetting error. Show your work. Master Mix Conc. Of Stock Final Number of Total µL µL/Rxn (total per tube) Conc. Reactions needed for solution master mix PCR buffer 50X 1X Water DNTP mix 100 mM 200 μΜ MgCla 24 mM 25 mM

Complete this Master Mix table for 8 DNA samples, a positive control, negative control, and an extra reaction for pipetting error. Show your work. Master Mix Conc. Of Stock Final Number of Total µL µL/Rxn (total per tube) Conc. Reactions needed for solution master mix PCR buffer 50X 1X Water DNTP mix 100 mM 200 μΜ MgCla 24 mM 25 mM

Comprehensive Medical Terminology

4th Edition

ISBN:9781133478850

Author:Jones

Publisher:Jones

Chapter21: Oncology (cancer Medicine)

Section: Chapter Questions

Problem F1CRE

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