Comparing SNPs to STR markers, which of the following are true? Select one: a. SNP loci generally have more alleles than STR loci. b. SNP loci could not be used for forensic CODIS DNA profiling. c. SNPs are not the same as microsatellite DNA. d. There are many more STR loci in the human genome than SNP markers.
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Comparing SNPs to STR markers, which of the following are true?
SNP loci generally have more alleles than STR loci.
SNP loci could not be used for
SNPs are not the same as microsatellite DNA.
There are many more STR loci in the human genome than SNP markers.
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- you performed a sanger sequencing on a particular segment of DNA. Explain at the molecular level what your gel profile would look like due to this mistake. a. you forgot to add ddNTPs to your test tube. b. you added too much ddNTPs to your test tubea. It is possible to perform DNA fingerprinting withSNPs instead of SSRs as DNA markers, but ingeneral you would need to examine more SNPmarkers than the 13 SSRs used in the CODIS database to be sure of a match. Explain why.b. DNA fingerprinting has been used to verify pedigrees of valuable animals such as show dogs, racinggreyhounds, and thoroughbred horses. However, thetechnology is much harder to apply in these casesthan it is in forensic applications for humans. In particular, many more DNA markers must be examinedin domesticated animals to establish the identity orclose familial relationship of two DNA samples.Why would you need to look at more polymorphicloci in these animals than you would in humans?Choose the one answer that fits best. Which of the following statements regarding DNA Fingerprinting is NOT correct (videos)? a. Fingerprinting is a technique that allows us to sequence an entire genome b. Forensic DNA profiling uses 13 different regions of DNA (according to the video) c. STRs allow us to tell individuals apart d. Short tandem repeats are in the non-coding regions e. DNA Fingerprinting is used in Forensic Sciences
- Provide the role of the following in the crude DNA extraction:a. salt b. cold alcohol c. cold distilled water d. blending or blender methode. liquid detergentMatch the following terms with their definitions and label each component of the PCR mixture in the diagram (use the letters A-D):I. DNA polymeraseII. PrimersIII. NucleotidesIV. Genomic DNA template A. DNA that contains the target sequence that will be replicated using PCR.B. An enzyme that copies the DNA sequence.C. A mixture of 4 nucleotides (A,G,C, and T) that will be polymerized into the replicated DNA sequence.D. A short DNA sequence that allows the enzyme to bind and initiate polymerization.A region of the genome is amplified by PCR to analyze two closely linked SNPs. PCR products from individual A were labelled with a red-fluorescing dye and those from individual B labelled with a green-fluorescing dye. These PCR products were mixed and hybridized to an oligonucleotide microarray as shown in the figure below. What can we conclude about the data shown? Select all true: a. individual A is heterozygous for the M1 and M2 haplotypes, individual B is heterozygous for M4 and M5 b. individual A is heterozygous for the M1 and M2 haplotypes, individual B is heterozygous for M3 and M6 c. Sanger sequencing traces of these PCR products would show the same double peaks at the same positions for these two individuals d. analyzing this PCR amplified region with conventional Sanger sequencing would be more accurate than the microarray analysis e. individuals A and B could be siblings who share a parent that is homozygous for one of the haplotypes {urgent}
- The figure below represents the size of various SSRS that are used for forensic analysis. The bars corresponding to each locus represent the range of size of the various alleles of the locus. Based on the figure, Which loci could result in a PCR product 200 bp in length? Select all correct answers. 100 bp 200 bp 300 bp D8S1179 D21S11 D7S820 CSF1PO D3S1358 TH01 D13S317 D16S539 D2S1338 D19S433 VWA TPOX A TPOX VWA D5S818 III D8S1179 FGA D21S11 O CSF1PO D18S51 FGA 400 bp ||||IDNA polymorphisms can be used as DNA markers. Describe the differences between SNPs and STR polymorphisms. How can these markers be used for DNA-mapping studies?Explain why DNA ladders are usually included during gel electrophoresis. One aspect of PCR that can be modified is the annealing temperature. In general, higher annealing temperatures show more specificity towards a single template, whereas lower annealing temperatures show less specificity and may bind to multiple regions throughout the genome. Discuss how using an annealing temperature that is too high or too low might influence the results of a PCR assay (and gel electrophoresis results) such as the one used in this study.
- Consider the following DNA sample: 5' CCGAGAACTTA 3' Draw a gel showing the following: 1. Sanger sequencing (ddNTP's): a. Lane 1: A pre-made ladder with the following molecular weights: 2, 5, and 9 b.p. Draw and arrow and label each band for your ladder. b. Lane 2: PCR of sample using dGTP, dATP, dCTP, and dTTP c. Lane 3: PCR of sample using ddGTP, dATP, dCTP, and dTTP d. Lane 4: PCR of sample using dGTP, ddATP, dCTP, and dTTP e. Lane 5: PCR of sample using ddGTP, ddATP, ddCTP, and ddTTPWhat is the most challenging issue facing genome sequencing? a. the inability to develop fast and accurate sequencing techniques b. the ethics of using information from genomes at the individual level c. the availability and stability of DNA d. all of the aboveIn primer designing, which of the following statements is correct? a. Primers should be 18-24 bases in length. b. Base composition should be 45-55% (G+C). c. Melting temperatures between 55-70°C are preferred. d. All choices are correct.