Cite the purpose of each of the following reagents in a differential stainingprocedure.a. Primary stain:b. Mordant:Lab reportExPEriMEnt9 90 Experiment 9: lab reportc. Decolorizing agent:d. Counterstain:
Q: (a) Which would be easier to prepare bacterial smear from, liquid or solid culture? Why? (b) Aside…
A: Introduction A bacterial smear is a small layer of bacteria that has been stained on a slide. A…
Q: Let's assume that you were teaching the Gram stain to four students, and each student did one thing…
A:
Q: write the protocol for Hexamine silver staining, including their reagents, materials, prinicple,…
A: Grocott’s alkaline hexamine-silver solution undergoes reduction to create precipitates of silver…
Q: Laboratory Scenario Analysis. Read the following statements. Fill-in the possible effect of the…
A: Biosafety refers to the application of knowledge, techniques, and equipment to prevent exposure to…
Q: components of a Romanowsky stain?
A: NOTE:- "As you have posted multiple questions under one, we will solve the first part for you, to…
Q: If the pH indicator were left out of MacConkey agar, the medium would bea) complex.b)…
A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…
Q: For post mortem stain to occur ______must happen. A. Coagulation B. Hemolysis C. refridgeration…
A: For post mortem stain to occur pH shift to alkaline must happen.
Q: The sediment observed in stained blood smear caused by: a) Overstraining b) More Haematoxylin in the…
A: Here we have to explain about why sediment observed in stained blood smear.
Q: What are Romanowsky stains? Name four of these. b: What are the components of a Romanowsky stain? b:…
A: According to bartleby expert guidelines, when multiple sub part questions are posted we are allowed…
Q: Microbiology
A: Gram staining is a technique which is used to identify the bacterial cell type, whether it is gram…
Q: Why must heat or a surface-active agent be used with application of the primary stain during…
A: During acid fast staining first of all heat is used to fix the bacterial smear onto the slide.…
Q: Let's assume that you were teaching the Gram stain to four students, and each student did one thing…
A: Gram staining may be a staining strategy utilized to distinguish and recognize bacterial living…
Q: Assume that, during the performance of this exercise, you madeseveral errors in your spore-staining…
A: The usual staining procedure of microorganisms involve the following steps: First step involves…
Q: Which of the following are proper disinfection steps? Check all the O Remove organic matter O…
A: Introduction: Disinfection is substances that are applied to non-living objects to destroy…
Q: 1. a) What are Romanowsky stains? Name four of these. b: What are the components of a Romanowsky…
A: 1) a. Romanowsky staining is a prototypical staining technique which is also known as…
Q: Select all of the following that represent examples of positive stains. A) nigrosin B) methylene…
A: Staining is a technique usually employed to enhance the contrast of specimens in microscopy. Many…
Q: .Leifson's stain is made up of tannic acid, basic fuschin stain prepared in alcohol base .A .B F .c…
A: Leifson's stain is a method used to identify the infections of the Helicobacter pylori bacterium.…
Q: A. Discuss the procedure and give the advantages and limitations of: 1. Kato - Katz Technique 2.…
A: Several techniques can be employed in the laboratory to find out or diagnose the specific disease or…
Q: Put the numbers in the space provided to indicate the order of Capsule Staining. If the statement is…
A: The primary function of capsule staining is to differentiate capsular material from bacterial cells.…
Q: A medical technician has recieved a tissue sample from the stool of a patient. The doctor believes…
A: A Gram stain is a test that checks for bacteria at the site of a suspected infection or in certain…
Q: Explain the reason for the effect on mass spectroscopy method of diagnosis by culture conditions.
A: Mass Spectrometry is an analytical method. It is used to quantify known materials to identify…
Q: 1.)What is the purpose of a counterstain?
A: BASIC INFORMATION ENZYMES They are the catalyst. They help in accelerating the chemical reaction.…
Q: Solid media is usually prepared with a 1-2% final concentration of agar. If these plates were…
A: Solid media is the type of media that is made up of liquid substances like distilled water and other…
Q: The following figures are the photomicrographs of the samples taken with Kern&Sohn Camera Microscope…
A: Ans: Lugols solution: The Lugols solution is 2.5 to 25% weight by volume total iodine.
Q: What are the principle and basic concepts of Simple staining
A: The simple staining doesn't give a lot of data about the cell separated from the bacteria'…
Q: The significance of using immersion oll when using 100X objective lens is: It will increase the…
A: Answer. Microscopy is a technique for making very small things visible to the unaided eye. An…
Q: Which physical or chemical agent would be the best choice for sterilizing the following items: glass…
A: Sterilization is a process used in a laboratory setting to remove, kill, or deactivates all forms of…
Q: Give three situations where the negative staining procedure would be used.
A: Negative staining is a specimen preparation technique that requires an acidic dye such as India Ink…
Q: write the protocol for mann's methyl blue-eosin staining, including their reagents, materials,…
A: Reagents: Alkaline alcohol reagent –100ml of absolute Ethanol or Methanol containing 0.2ml 0.1N…
Q: Give the drug examples of emulsion prepare by these method:- 1)dry gum method 2)wet gum method…
A: Emulsions are heterogeneous biphasic systems. Thus, there are two immiscible phases. One of the…
Q: Distance of color diameter (mm) 20 15 KMNO, 0.1M - KMNO, 1.0M - 5. 15 45 09 75 Time (minutes) 06
A: * The molecules movement in agarose gel is dependent on the size charge *And pore size also…
Q: You can perform a specific staining method to confirm the purity on the culture. Describe this…
A: Staining methods are used to differentiate between two bacteria depends on their cell wall. To…
Q: Name the frequently used stains in blood smear preparation. Describe briefly and give their specific…
A: Any deviation from the normal is the diseased condition. The disease is caused by many reasons.…
Q: Blood is stained with stain. a) Methylene blue b) Safranin c) Leishman stain d) Carbol fuchsine
A: Introduction - A blood smear, also known as a peripheral blood smear or blood film, is a thin…
Q: acillus cereus bacteria, in staining reaction tests, such as A.simple staining B.gram staining C.…
A: Answer
Q: Andrew has to prepare 30 plates (use maximum volume), 15 slants (small test tube), and 15 stabs (big…
A: Petri plates, slants and stabs of Culture media such as Luria Bertani agar is used in microbiology…
Q: Identify the FALSE statement about visualization of thin layer chromatography results. A. The spots…
A: Introduction:- Thin Layer Chromatography is a method for separating non-volatile substances. The…
Q: Write the instructions, reagents, principles and the procedures for the following. 1. Periodic acid…
A: (AS PER OUR GUIDELINES WE ARE PROVIDING YOU ANSWER OF 1ST TWO QUESTIONS. YOU CAN POST OTHER…
Q: If you prepared a tube with 10-2 dilution and from this 0.1 ml ia taken into 9 ml medium, the new…
A: Given Initial concentration 10-2 Volume taken = 0.1ml Volume added = 9 ml Find final dilution…
Q: Write down the materials, reagents, Principle, procedure and results for Mann’s methyl blue-eosin…
A: Polychromatic solution of Methyl Blue and Eosin Y stains (modified Mann's solution) is used for…
Q: Gram straining procedure. Indicate the following information A. Shape........ B. Stained color…
A: On the basis of gram staining, we can differentiate between two types of bacteria, one with thick…
Q: 2. Give the purpose of each of the following reagents in a differential staining procedure: (Primary…
A: Differential Staining uses multiple chemical stains to view the microscopic organisms. It is because…
Q: at is the wavelength of maximum aborbance for the Bradford reagent in its blue form. at is the…
A: Bradford reagent in Bradford assay is used to to assess the amount of protein in a sample. The…
Q: Describe the procedure for performing a Gram stain. ▪ What is the procedure? ▪ How does each…
A: Bacteria are ubiquitous microscopic , one celled organism which possess variety of shape ,…
Q: Give the three disadvantages of using irradiation.
A: Irradiation is a comparatively newer technology of food preservation in which food item is…
Q: differential staining procedures
A: Differential staining : Differential staining is one of the important staining method. In this…
Q: In the conduct of serial dilution, which of the following tools is NOT necessary? a. Agar…
A:
Q: The Duquenois–Levine test is a valuable color test for___________.
A: In a forensic chemical analysis of an unknown material, the analytical method is to narrow down the…
Cite the purpose of each of the following reagents in a differential staining
procedure.
a. Primary stain:
b. Mordant:
Lab report
ExPEriMEnt
9
90 Experiment 9: lab report
c. Decolorizing agent:
d. Counterstain:
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- Neutralization of residual disinfectant could be made by: a. Dilution if the concentration exponent is high b. Dilution if the concentration exponent is low c. Adding specific agent e.g. 3% Tween 80 d. Either a or c e. Either b or cGive the procedure of Sabin-Feldman dye test. Describe the positive and negative results.write the protocol for Hexamine silver staining, including their reagents, materials, prinicple, procedures results, and cautions
- 1. What is the usual stain used for staining a sputum smear? 2. Give the principle of acid fast staining and gram staining. 3. Explain the Bethesda System. 4. How will you instruct a patient that will undergo a brochoscopy?Write the protocol for lactophenol blue stain method indicating; materials, reagents, Principle, procedure and results.1. Why must heat or a surface-active agent be used with application of the primary stain during acid-fast staining?2. Explain the importance of using methylene blue as the counterstain in the acid-fast staining method. 3. What is the specific diagnostic value of this staining procedure?4. What is the specific diagnostic value of this staining procedure?5. Explain the importance of using methylene blue as the counterstain in the acid-fast staining method.6. What is the specific diagnostic value of acid fast staining procedure?
- Briefly explain how the following can affect the quality of stained blood film; I) .The size of the blood drop used for the preparation of the filmi ii. A delay in smear preparation III). The angle of spreadiv). humidity of the laboratory environmentv). Pressure 2. Describe five characteristics of an ideal smearGive a vivid outline of the Standard operating procedure of neutralization test.Write the instructions, reagents, principles and the procedures for the following. 1. Periodic acid Schiff technique 2. Grocott's hexamine silver technique 3. Ziehl-Neelsen stain 4. Lactophenol blue stain 5. Mehtamine silver technique 6. Shikata's orcein method 7. Mann's methyl blue-eosin stain
- The acid fast technique is also known as: A. Ziehl-Neelsen stain B. Auramine-rhodamine stain C. Gerrard-Lampard Stain D. Kinyoun method2. Give the purpose of each of the following reagents in a differential staining procedure: (Primary stain, Counter stain, Decolorizing agent and Mordant)explain the rationale or theoretical basis for differential staining procedures