Chosen wavelength : 400 EDTA Volume (ml.) Absorbance O ml. 0.445 I ml. 0.439 2 ml. 0.434 3 ml. 0.428 4 ml. 0.388 5 ml. 0.352 6 ml. 0.351 7 ml. 0.320 8 ml. 0.289 9 ml. 0.275 10 ml. 0.263 I1 ml. 0.256 1.Explain why the selected wavelength was chosen for inspection of the titration. 2. Draw the titration curve of the titration.
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- A protein’s concentration can be determined spectrophotometrically by mixing it with an indicator called a Bradford reagent and measuring the amount of light absorbed by the protein. Use the plot below to determine the mass of protein (in micrograms, μμg ) in 100.0 mL of the solution if its absorbance is 0.398. A. 52 B. 3.1 C. 2.4 D. 0.52 E. 0.031Prepare a 1:200 dilution of the 20 mM Coomassie solution to measure the max wavelength—you need 2 ml for the absorbance measurement but the dilution does not matter as long as the absorbance is in the range of 0.2 to 0.6. Set the initial wavelength at 450 nm. Blank the spec with a tube containing 2 ml water. Use the 1000 ul pipette for water because you need to deliver the same amount of water in each tube for the next exercise. Why is it when the calculations are done for the given values above, the value for ml is 0.01 for coomassie blue?4. Determine the percent transmittance of ß-carotene, C40H56 that has an absorbance of 0.5 when analyse using a UV-Visible spectrophotometer. A. 6.83% B. 31.6% C. 0.32% D. 68.3% 5. The equation of Beer Lambert's Law is A = abc. If the 'b' is distance, 'a' is absorptivity, and 'A' is absorbance, what does the 'c' represent? 7. A. Intensity B. Analyte concentration C. Analyte transmittance D. Frequency 6. All the following is an example of photon detectors EXCEPT: A. Vacuum phototube B. Silicon photodiodes C. Bolometers D. Photomultiplier tubes C=0 bond stretch occurs at 1750 cm¹ and C-O stretch occurs at 1100 cm¹ because: A. The C-O has a single bond which is a stronger bond, so it vibrates at a higher frequency than the C=O. B. The C-O has a single bond which is a stronger bond, so it vibrates at a lower frequency than the C=O. C. The C=0 has a double bond which is a stronger bond, so it vibrates at a lower frequency than the C-O. D. The C-O has a double bond which is a stronger bond,…
- 15. Lenses are coated with thin film to reduce a. Diffraction d. Refraction 10. Which of the following produces an interference pattern with a broad central bright fringe with a width twice that of the secondary maximum? a. Crystalline solid c. Double slit b. Polarization C. Reflection b. Diffraction granting d. Single slit 17. What is the effect of increasing the number of lines ruled on a diffraction grating? a. The spectrum produced will be broader. b. The interference pattern will be less sharp. c. Only shorter wavelengths can be diffracted. d. The distance between the slits will be increased. 18. Crystal structures are analyzed using a. Diffraction grating C. Single slit 19. Which of the following will increase the separation between the dark fringers in the diffraction pattern produced by a single slit? a. Decreasing both the wavelength of light and the width of the slit by the same amount. b. Increasing the wavelength of light used and the width of the slit by the same amount,…s with our subject matter experts now. arrow_forward Question The amount of protein in a sample is measured by the samples absorbance of light at a given wavelength. Using standards, a best fit line of absorbance vs. mg protein gave the following parameters: m = 0.01630 sm = 0.00022 b = 0.1040 sb = 0.0026 An unknown sample has an absorbance of 0.246 ± 0.0059. What is the amount of protein in the sample?During your experiment, you measure the absorbance of a sample to be 1.362. You have to convert thisabsorbance to methylene blue concentration (in ppm) using the absorbance measurements of two standardsolutions of known concentration:5 ppm: Absorbance 0.55510 ppm: Absorbance 1.211What is, in ppm, the concentration of your sample? Report using 3 decimal figures. Your answer must becorrect to 0.02 ppm
- For my lab I had to find the unknown concentration of a solution. I found wavelength max of the potassium chromate. Then used that max to find the absorbtion for my sample dilutions. Based on my attached graph. Is my unknown concentration # making sense? Or did I make an error with the absorbance I wrote down in the lab. I did my calculations when I got home. They are correct, but my unknown concentration amount doesn't look like it will even be on the graph. Or am I wrong. Please advise and explain. Thank youWhat absorbance value corresponds to 40.0% T? A = A 0.0109 M solution of a certain analyte exhibits 40.0% T at a wavelength of 280 nm. What will the percent transmittance be for a 0.0283 M solution of the same analyte? Assume the pathlengths for each measurement are equal. %T =A. dependent variable independent variable B) Graph B C) Graph C D) Graph D E) Graph E D. In 700 wavelength (nm) 400 B. of 700 400 wavelength E. Which graph above best illustrates the relationship between the concentration of a substance and the height of its absorbance peak? A) Graph A C. time time
- 5. Determine the percent transmittance of B-carotene, C40H56 that has an absorbance of 0.5 when analyse using a UV-Visible spectrophotometer. A. 6.83% B. 31.6% C. 0.32 % D. 68.3% 6. The equation of Beer Lambert's Law is A = abc. If the 'b' is distance, 'a' is absorptivity, and 'A' is absorbance, what does the 'c' represent? A. Intensity B. Analyte concentration C. Analyte transmittance D. Frequency 7. All the following is an example of photon detectors EXCEPT: A. Vacuum phototube B. Silicon photodiodes C. Bolometers D. Photomultiplier tubes 8. C=O bond stretch occurs at 1750 cm1 and C-O stretch occurs at 1100 cm1 because: A. The C-O has a single bond which is a stronger bond, so it vibrates at a higher frequency than the C=O. B. The C-O has a single bond which is a stronger bond, so it vibrates at a lower frequency than the C=O. C. The C=O has a double bond which is a stronger bond, so it vibrates at a lower frequency than the C-O. D. The C=O has a double bond which is a stronger bond,…What absorbance value corresponds to 47.4% T? A = 0.323 A 0.0141 M solution of a certain analyte exhibits 47.4% T at a wavelength of 280 nm. What will the percent transmittance be for a 0.0279 M solution of the same analyte? Assume the pathlengths for each measurement are equal. %T = Incorrect %A commercial drink mix is prepared at a concentration of 0.1 M and placed in a spectrophotometer and is measured to have an absorbance of 0.36. Finding the drink too watery, more drink mix is added, increasing the concentration to 0.15 M. What is the absorbance of the new solution?