CASE STUDY 2 Alette, a 17-year-old Haitian woman, presented to a women's clinic complaining of watery and foamy bowel movements 5 to 10 times a day. She had also experienced abdominal cramps and a low-grade fever. The physician ordered a stool sample for routine O&P as well as for culture and sensitivity tests. The culture and sensitivity tests were reported as negative for enteric pathogens. The laboratory technician performed a routine O&P examination, including a permanent trichrome stain. No parasites were observed. Two days later, a repeat O&P was ordered. The patient was now diagnosed as HIV- positive. The repeat sample was again examined for ova and parasites by routine laboratory procedures. This time, the technologist noted oval forms of the wet preps that were highly refractile and suggestive of fungal cells, but no budding was seen. The trichrome permanent stain did not confirm the laboratory technician's suspicions. Not satisfied that the sample was negative, the laboratory technician performed an alternative permanent stain. The organisms seen on this stain are illustrated in the diagram. These organisms were noted as 5 μm in size with one to six dark granules within their cytoplasm. 1. What organism did the laboratory technician suspect, and why? What morphologic form is seen in this diagram? 2. What was the alternative permanent stain, and how would these parasites appear following this stain procedure? 3. What fungal elements might appear similar to this organism? How would they stain with the alternative stain?

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Alette, a 17-year-old Haitian woman, presented to a women's clinic complaining of watery and foamy bowel movements 5 to 10 times a day. She had also experienced abdominal cramps and a low-grade fever. The physician ordered a stool sample for routine O&P as well as for culture and sensitivity tests.

The culture and sensitivity tests were reported as negative for enteric pathogens. The laboratory technician performed a routine O&P examination, including a permanent trichrome stain. No parasites were observed. Two days later, a repeat O&P was ordered. The patient was now diagnosed as HIV-positive. The repeat sample was again examined for ova and parasites by routine laboratory procedures.

This time, the technologist noted oval forms of the wet preps that were highly refractile and suggestive of fungal cells, but no budding was seen. The trichrome permanent stain did not confirm the laboratory technician's suspicions. Not satisfied that the sample was negative, the laboratory technician performed an alternative permanent stain. The organisms seen on this stain are illustrated in the diagram. These organisms were noted as 5 um in size with one to six dark granules within their cytoplasm.