Briefly compare the following test systems, Micro-tox, ATP-Tox, and Daphnia magna IQ test . Explain how each assay works (the principle), not the test procedure.
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Briefly compare the following test systems, Micro-tox, ATP-Tox, and Daphnia magna IQ test . Explain how each assay works (the principle), not the test procedure.
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- Discuss the medical application of the Benedict’s test? What other test(s) are used in parallel to Benedict’s test?Calculation for a neutral red assay please include steps since it is essential for the report and it is marked.With detail, compare and contrast the following 5 real-time assays; Taqman, SYBR Green, Molecular Beacon, FRET, and Scorpion. Please write complete sentences in paragraph form. Identify how the assays are similar but more importantly, what are the distinguishing features of each of the technologies.
- 3) Were all of the conditions of a standardized Kirby-Bauer test met as you performed this assay? If not, which were not? 4) What is the significance of colonies that develop within otherwise clear zones of inhibition? If the laboratory report for one of your patients indicated colonies within the zone, what concerns would you have for your patient?1.a)What is the equivalence point and how does it relate to the recommended proportion of serum to blood in the heme agglutination assay? b. what would the predicted outcome be if you used too little serum in this assay? Why? c. what would the predicted outcome be if you used too much serum in assay? Why?Discuss and explain the results of this graph. Graph shows results of WST-1 assay. T0 ( time zero) shows assay performed in order to obtain an absorbance at the time of test agent added in order to determine 1. how much cells have grown over the incubation period 2. Growth inhibition by test agent 3. If test agent caused cytotoxicity
- Discuss what for is the acrolein test and the principle behind the test.Construct a schematic diagram on how ACE inhibition assay is performed based on the protocol given. Explain how the colorimetric method measures ACE inhibitory activity. Protoc reference:Which of the following statements is/are FALSE about Bradford assay? 1. Upon addition of the reagent, the color of the protein solution becomes purple. II. The absorbance of the resulting solution is measured at a wavelength of 540-550 nm. Both I and II O II only Neither I nor II O I only