1. One (1) mL of spring water were added to a petri dish to which 30 mL of melted Plate Count Agar were added. After incubation, 38 colonies arose on the plate. What was the count of CFUs per mL of the spring water?
Q: What property of carbon makes it essential for organic life?
A: The fundamental biomolecules of life, such as carbohydrates, proteins, lipids, and nucleic acids,…
Q: Baltimore classification Characteristics (dsDNA, ssDNA, dsRNA, ssRNA (-),ssRNA (+), ssRNA RT, ds DNA…
A: Baltimore classification is based on the viral genome, whether it is made of DNA or RNA, whether the…
Q: Discuss the structures of the following organisms when viewed under the microscope: Scolex of…
A: Tapeworm Belongs to the class of eucestoda of platyhelminthes . They all are endoparasites. They…
Q: Question Antibiotics and antibacterial products have saved a countless number of lives. With all the…
A: Evolution changes the existing traits or gives rise to new traits or species.
Q: What is the importance of heritability in natural selection?
A: Introduction In breeding and genetics, the term "heritability" refers to a statistic that…
Q: hoose the appropriate type of media for the development of Lac- ello
A: The nutrition medium known as minimal media is one that has the fewest nutrients necessary for…
Q: Name the structures of the central nervous system and the peripheral nervous system and describe…
A: Introduction The extensive network of neurons that move throughout the body makes up the nervous…
Q: To what kind of injuries are cells susceptible?
A: Cell injury is basically defined as a sequence of events that occur when the stresses in the cell…
Q: What role do mutations play in the fragment presented above? How is the mechanism of natural…
A: The abrupt changes in the DNA sequence is called mutation.Iit could be beneficial, harmful or…
Q: You are doing a genetics experiment with the fruit fly. In the "F1" generation, you cross two…
A: The alleles are the alternative forms of a gene that are located on the same locas of a homologous…
Q: Lipopolysaccharide (LPS) is a major feature of which cell wall? O Fungal O Viral O Gram-positive…
A: Lipopolysaccharide are large molecules consisting of a lipid and a polysaccharide. They are found as…
Q: 5. Very happy Escherichia coli cells reproduce on a 20 minute time frame (doubling or generation…
A: Doubling time is the time in which the cells of E.coli double in their number by undergoing cell…
Q: Fill in the space with the appropriate word from the word-bank below; causing During inhalation, the…
A: Inhalation is the process by which we take in oxygen-rich air. Exhalation is the process by which we…
Q: Which one of the options shown below is a common mechanism of entry in some enveloped viruses?…
A: Viruses are tiny infectious agents that can cause a wide range of diseases in humans, animals, and…
Q: detail three microscopic techniques, one of which should be the FISH technique, which can be used to…
A: Microscopy is the most essential invention that covers a multiple processes and its detection. This…
Q: The proton pump in ETC is an active transporter. Where does it get the energy to pump protons…
A: In aerobic cellular respiration there are four steps. In the first step glucose is converted into…
Q: Which of the following about basophils and mast cells is true? 1) basophils make leukotrienes, and…
A: Introduction: a particular sort of blood cell that is produced in the bone marrow and present in…
Q: n plants known as “morning glory”, the allele for the dominant red-flower color is incompletely…
A: Given that, dominant red flower is incompletely dominant over recessive white colored flower. In…
Q: What are the major cellular structures and their functions?
A: A subfield of biology called cell biology examines the composition, operation, and behaviour of…
Q: WT A B с D E F G H WT A + + B + C DE + + + + F + G H + + + The above complementation table analyzes…
A:
Q: Mutation in the operator that reduces the affinity of the operator for the repressor protein…
A: Lac operon is an inducible operon. When lactose is present in medium, then lac operon starts.
Q: How can the yeast vector, YEP, containing your desired gene, be inserted into the host cell? Discuss…
A: YEp stands for Yeast Episomal plasmid is a shuttle vector. Shuttle vectors are vectors designed in a…
Q: hat are some of the challenges of using recombinant molecules to treatment of HIV?
A: HIV (human immunodeficiency virus) affects immune cells that aid in the body's ability to fight…
Q: Which of the following would allow for communication between neighboring cells, so that the involved…
A: Introduction Cell signalling, sometimes referred to as cell communication, is a cell's capacity to…
Q: The Choose... bases of the two strands. On the leading strand, Choose... enzyme attaches to DNA and…
A: DNA replication is a process by which new daughter DNA molecule is produced from the parental DNA.…
Q: Mapping a new mutation on the same chromosome as the mapping genes: Scenario 1 1. If the m mutation…
A: The genotype is the genetic makeup of an individual, while the phenotype is the physical appearance…
Q: Do phantom perceptions arise from erroneous neural signals from an amputated stump, or from residual…
A: This phenomenon is related to the conscious feeling of the presence of a missing body part like a…
Q: The features of the garden pea that made it a basic principles of inheritance •good organism for…
A: Introduction Inheritance is the way by which genes or traits from one generation are passed on to…
Q: Answer as Directed. Below is the model of a lac operon. lac I lac Z с promoter operator lac Y lac A…
A: According to our guideline we can answer only the first 3 subparts of the question. So, upload the…
Q: What cellular signaling molecule (mediator) is released to activate B lymphocytes at stage 2". ●…
A: B lymphocytes are the cells of immune system which will fight with pathogens via the formation of…
Q: In pea plants Red (R) flower color is dominant over white (r) flower color and green seeds (G) are…
A: Introduction Genetics makes a distinction between genotype and phenotype. An organism's entire…
Q: Angiostrongyliasis cantonesis, the organism that cause Rat Lungworm Disease, is a
A: Answer: A parasitic organism is an organism which lives on or in other host organisms or get their…
Q: Eukaryotic messenger RNA includes a poly A tail. When the poly(A) tail is less than approximately 30…
A: Introduction Messenger ribonucleic acid (mRNA) is a single-stranded RNA molecule that, in molecular…
Q: Use the following information to answer the next question. The end product of glycolysis reacts…
A: The process of converting chemical energy from nutrients into adenosine triphosphate in an…
Q: If a woman is heterozygous dominant and her husband is recessive for a genetically inherited trait…
A: A diploid organism having two different types of alleles is said to be heterozygous. Individuals…
Q: Choose the best combination of depyrogenation methods: 0.45 um filtration +0.22 µm filtration…
A: Introduction Pyrogens are the substance that causes pyrexia( a type of fever). The gram-negative…
Q: How are different touch receptors distributed over the body?
A: We realise that we have uneven distribution of touch receptors in the skin; some locations have many…
Q: Explain how PCR/OLA (polymerase chain reaction/oligonucleotide ligation assay) can be used in the…
A: Introduction Sickle cell disorder is a rare condition in which the red blood cells of the individual…
Q: 15-16. In the fruit fly, vestigial (tiny) wings and hairy body are produced by recessive genes. The…
A: Drosophila is a genus of flies in the family Drosophilidae. Because many species tend to stay around…
Q: 5. Very happy Escherichia coli cells reproduce on a 20 minute time frame (doubling or generation…
A: Given here are 2 e coli cells that have reproducing time of 20 minutes we have to calculate…
Q: Answer as Directed. Below is the model of a lac operon. lac I lac Z C promoter operator +1 lac Y lac…
A: The prokaryotic gene regulatory system is known as operon system which ultimately responsible for…
Q: a. which data represents the chloroplast? explain. b. which organelle is more at risk to changing…
A: DNP that is also known as Dinitrophenol is act as a protonophore which allow the photons to leak…
Q: The procedure to find the mechanism through which bacterial genetic material may withstand…
A: Small, microscopic organisms known as microorganisms are found almost everywhere. They are found in…
Q: Yeast cells are added to sterilized corn mash in large industrial fermenters to produce ethanol,…
A: Fermentation is a metabolic mechanism in which a carb, such as starch or sugar, is converted into an…
Q: reasonable answers. 7. Speculate (guess) on why there were more three year olds than two year olds…
A: Perch or Perca fluviatilis is commonly known as European perch, redfin perch, English perch, etc.…
Q: 1. Show the different kinds of gametes which can be formed by individuals of the following…
A: Genotype is genetic makeup of organism. So, in this question we need to determine the gametes from…
Q: Describe how hroughout the Games, performance-enhancing drug testing became more rigorous and…
A: Performance enhancing drug test in sports competition has became a common practice. Athletes use…
Q: please please help me do this question English translation is below
A: jej
Q: 3. One repair mechanism for double-strand breaks involves the unwinding of the damaged DNA followed…
A: DNA repair is the correction of incorrect nucleotides or missing nucleotides in a single strand or…
Q: How environment effect the living organisms?
A: Cells make up a live entity. Because of the rise in cell number, cells divide as well as the…
1. One (1) mL of spring water were added to a petri dish to which 30 mL of melted Plate Count Agar were
added. After incubation, 38 colonies arose on the plate. What was the count of CFUs per mL of the spring
water?
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- Ten grams of hamburger were added to 90 mL of sterile buffer. This was mixed well in a blender. One-tenth of amL of this slurry was added to 9.9 mL of sterile buffer. After thorough mixing, this suspension was further dilutedby successive 1/100 and 1/10 dilutions. One-tenth of a mL of this final solution was plated onto Plate Count agar.After incubation 145 colonies were present. How many colony-forming units were present in the total 10 gramsample of hamburger?1. One gram of sludge was added to a 99 mL dilution blank. Three 1/10 dilutions were then made. From the last dilution made, 200 µL was plated onto an all-purpose medium. After incubation, 123 colonies were counted. What is the CFU/mL in the sludge? Note: 1 gram sludge is equal to 1 mL sludge.one lm of milk was added to 9 ml of sterile saline. 0.1ml of the diluted sample was put into a petri dish with melted agar. How many cfu/ml are in the milk if 200 colonies were counted?
- 1. What is one advantage of utilizing the pour plate technique over the streak plate technique ? 2. Why must the agar pours be cooled to 45C before use in the pour plate technique? 3. Explain the consequences if a group removed all the agar pours from the water bath at one time and allowed them to sit on the bench for several minutes before using them. 4. Why can the agar pour tubes be rinsed in the sink after the agar is transferred to the Petri plate ? Could you rinse the tubes if the bacteria had been pipetted into the agar pour tubes rather than in the plates? Explain. 5. What would be the result if a student dipped his / her loop in the stock culture during inoculations of each quadrant ? Explain . part B 1. The introduction stated that microbes are mechanically separated or diluted over the surface of the medium . How is this accomplished ? 2. Go to https://commons.wikimedia.org/wiki/File:MacConkey_agar_with_LF_and_LF_colonies . . Which side of the plate (left or right)…3. You were instructed to add 2.75 mL out of 5.0 mL of an undiluted sample to 125.25 mL of sterile diluent. Instead, you add all 5.0 mL to the 125.25 mL, What was the intended dilution and what was the actual dilution? ited States) E Focus OCT 30 W MacBook P DII DD F7 F8 F9 F10 F4 F5 F6 F3 2$ 4 081 R F G H. K < CO1. Calculate the CFU/mL of the original culture for the countable plate as shown in the diagram. b) How many colonies would you expect if you plated out 0.1 mL from Tube C. c) How many colonies would you expect if you plated out 1.0 mL from Tube C.
- Below is a diagram of the serial dilution of a culture with 1 x 10º cells. Fill out the missing information. 250 Culture Diluent 1 x 10° cells/mL 9 mL 9 mL 9.9 mL 9.9 mL 99.9 mL Volume to add to diluent Final Dilution level Theoretical count after plating 100 uLFive grams of soil were added to 45 ml of sterile water and shaken vigorously. After that, 0.1 ml of this was added to 9.9 ml of sterile water. This was further diluted by four successive 1/10 dilutions. The last dilution was used to prepare a spread plate. After incubation, 58 colonies were present on this plate. What is the CFU/g of the soil sample?1mL Stock #1 1mL 9mL #2 9mL #3 1mL A 9mL #4 0.1mL 1mL O O B 9mL #5 1mL 1mL 9mL #6 O Using the picture serial dilution scheme and the following information (plate A has 276 colonies, plate B has 298, plate C has 2, and plate D has 30), calculate the average number of colony forming units per mL in the stock tube. Make sure to only use countable plates. Round your answer to the nearest one. Write only the number with any needed commas or decimals. Do not include units. 0.1mL 1mk D O
- Serial dilution = previous dilution x dilution 11 mL [2] 3 mL Colony count: 100 0.1 mL sample [1] 9.9 mL 5 mL 0.1 ml. Cokony count Dilution: 10-1 10-3 [3] 150 Dilution factor (DF): [4] [5] [6] Compute for the CFU/mL of the sample. Show your solution and express your final answer to 2 significant figures.If each agar plate contained 20 ml of LB agar and had an ampicillin concentration of 150 ug/ml, what is the approximate amount of ampicillin (grams) in each plate?Three grams of soil from the ground were added to 27 mL of sterile water and shaken vigorously. After the soil particles settled, 0.1 mL of this was added to 9.9 mL of sterile water. This was further diluted by 4 successive 1/10 dilutions. One mL from the last dilution was used to prepare a pour plate. After incubation, 289 colonies were present on this plate. What was the number of colony-forming units/gram of the soil?