b. Using a thin line, graph the relative amount of DNA per nucleus using the LEFT Y axis (G1, S and G2 are graphed for you). c. Using a thick line or different color and the scale on the RIGHT Y axis, graph the changes in Ploidy or sets of information per nucleus. Haploid (1n) means one of each chromosome type. Diploid (2n) means two of each chromosome type.
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- 4. Draw and give the procedure of harada-mori technique.3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV. A. The cell is being viewed under high power. (the pink specimen picture belongs to this question) Scanning power objective=4x; Low power objective= 10x; High power objective= 40x; Eyepiece= 5x; Low power field of view (FOV)= 4.2 mm B. These cells are being viewed under high power. Use the length of just one cell to estimate the number of cells that can fit into the FOV. (the two yellow circle specimen picture belongs to this question) Scanning power objective = 5X; Low power objective = 40X; High power objective = 100X; Eyepiece = 10X; Low power field of view (FOV) = 1.5 mm1. How will the following affect resolution during microscopy?i. Closing or opening the diaphragmii. Raising or lowering the condenserii. Increasing or reducing the light intensity 2. During the calibration of an ocular micrometer, two (2) stage micrometer divisions were observed to line up with ten (10). If the graduation interval on the stage micrometer is 0.01mm, calculate: i. The calibrated value of the ocular micrometer in um.ii. The size of Pseudomonas aeruginosa which has occupied 5 ocular divisions. 3. What are the methods of staining?
- 3. Complete the information needed. Use the calibration factor from #2. CF = stage micrometer divisions subtended by om x value of one stage um division ocular micrometer divisions subtended by stage micrometer or CF = (1 su x 10μm) 10 ou CF = CF = 1μm Ocular scale Stage micrometer scale (stage units x 10μm) ocular units 20 30 40 50 60 70 80 90 100 I Name of Organism: Organism B Objective Used: 010 Total Magnification: 1000x Ocular Micrometer units (violet rods): 15 Length of Organism: Computation:6. What is meant by a control plate? 7. Which two are control plates? - 205- Al 1d X -3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV. A.The cell is being viewed under high power. Scanning power objective=4x; Low power objective= 10x; High power objective= 40x; Eyepiece= 5x; Low power field of view (FOV)= 4.2 mm
- 3. Explain why the specimen must be centered in the field of view on low power before going to high power.please help me make this procedure a flow chart III. Procedure: A. MicroscopyOperating Procedure:Place the microscope close to the edge of the table. Select a suitable stool so that when looking into the eyepiece, your back is straight and your neck is bent at the nape.1. Lower the body tube by turning the course focus knob until the 10x or 16mm objective reaches the downward stop.2. Look through the eyepiece and adjust the mirror to the position which provides the brightest and the most evenly illuminated field of vision which is the circular area seen in the eyepiece. Raise the condenser until its top lens at the same level as the stage. Place the slide on the stage and fasten it using the stage clips.3. Position the specimen area of the slide over the center of the stage aperture.4. Looking through the eyepiece, raise the coarse focus knob until the image appears. Focus as sharply as possible. Low power objective has a much greater depth of focus and is generally used for the…blackboardcdn.com/5bfc08ba3fldc/14683296?X-Blackboard-Expiration=16245468000008X-Blackboard- 19 / 47 100% 1.4. Functions of the light mlcroscope parts Complete the following table by writing the function(s) of each of the parts indicated. Structure Function Diaphragm / iris Stage opehing Lamp Objective lenses Eye piece Coarse and find adjustment knobs Stage Stage rack prt sc delete home backspace lock enter pause t shift
- 4. How can you avoid cross-contamination of samples? A. Use different sized micropipettors for each repeat of the experiment B. Change the tip after each transfer C. Always press the plunger softly D. Never turn the volume dial above or below the range of micropipettor3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV. b. These cells are being viewed under high power. Use the length of just one cell to estimate the number of cells that can fit into the FOV. Scanning power objective = 5X; Low power objective = 40X; High power objective = 100X; Eyepiece = 10X; Low power field of view (FOV) = 1.5 mm3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV. b.These cells are being viewed under high power. Use the length of just one cell to estimate the number of cells that can fit into the FOV. Scanning power objective = 5X; Low power objective = 40X; High power objective = 100X; Eyepiece = 10X; Low power field of view (FOV) = 1.5 mm