Q: What are the primary limitations imposed by using Hanging-drop method (or Wet mount) to observe…
A: Hanging drop preparation is a type of wet mount in which a drop of organism-containing media is…
Q: What is the difference between a mixed culture and a contaminated culture if they both contain more…
A: In microbiology , a bacterial culture is a growth of microorganisms which have been grown in a…
Q: Identify and describe the 4 wet-heat methods that are used to control microbial growth.
A: The different methods used for controlling the growth of microbes by moist heat are boiling,…
Q: Discuss why microorganism presents in the nature environment has a longer lag phase and shorter…
A: The microorganisms present in the environment are continually replicating and utilizing the…
Q: Why is it important to keep the culture at the correct incubation temperature when measuring the…
A: Generation time is the time which is taken by the bacteria to double in number during a specified…
Q: Day 1 Day 2 Day 3 Day 4 log CFU/ml 5.6294 5.6493 5.8488 5.8235 Data for Graph 1 Day 1 Day 2 Day 3…
A: Bacteria are prokaryotic organisms that may be pathogenic in nature. The cells are devoid of…
Q: Why do variations in generation time exist:a. Among different species of microorganisms?b. Within a…
A: Generation time is the time taken by a microbial species to double in number. This ranges from…
Q: What are the physical methods of microbial control
A: Control of microorganisms is fundamental to forestall the transmission of illnesses and disease,…
Q: How might an enrichment culture be used to isolate bacteria capable of growing photoautotrophically…
A: Answer: Introduction: Enrichment cultures can be able to rise a less quantity of required organisms…
Q: Why is the membrane filter method preferred to the most probable numbermethod (MPN) for the…
A: Most probable number (MPN) is a method used to estimate the numbers of bacterial cells in a water…
Q: How is the CTSA (Coconut tryptic soy agar) growth medium designed to meet the nutritional need of…
A: Coconut trypic soy agar media are general-purpose, non-selective media that provide enough nutrients…
Q: micro-organisms are the most resistant to heat sterilization?
A: Most of the bacteria cannot survive adverse heat condition. But somehow particular bacteria can…
Q: How does McConkey agar serve as both selective medium and differential medium ?
A: MacConkey agar is a culture media that contain bile salts, crystal violet dye, neutral red dye along…
Q: Why are complex media preferable to chemically defined media for routinecultivation of…
A: Microorganisms require nutrients as an energy source and certain other environmental conditions to…
Q: Why is the time of exposure and temperature required for sterilization greater for dry heat than for…
A: Any technique that removes, destroys, or deactivates all forms of life and other biological agents…
Q: What temperature is considered adequate for achieving sterilization of a compost pile?
A: temperature considered for achieving sterilization of a compost pile is 135-160 F. At a…
Q: What is differential medium and differential media? Explain the difference ?
A: Difference between differential medumium - .- A differential medium support the expansion of any…
Q: what criteria that a microorganism must meet before it can be considered an “indicator?”
A: Hygiene plays a major role in maintaining the health. It includes the quality of food, water, air,…
Q: among these five areas such as: air, faucet, doorknow, table, and floor, in the laboratory is…
A: Microorganisms are present everywhere in nature, they are ubiquitous in their dwelling nature ie…
Q: Name 2 indirect methods for measuring microbial growth and describe how each is performed. a. i.…
A: Two indirect methods for measuring microbial growth are : 1. Turbidimetric method 2. Standard plate…
Q: Compare and contrast moist and dry heat methods of microbe control and give 2 examples of each.
A: The growth of microbes in an inanimate object can lead to the contamination of the particular…
Q: what is the cause of different species of psychrotrophic bacteria growing in the CTSA medium at 7…
A: They can grow at extreme low temperatures and lead to spoilage of food by modifying the compounds…
Q: Is the autoclaving process absolute in killing all microorganisms? If not, what are the factors that…
A: Autoclave is an instrument or device which is used in method of moist heat sterilisation; while…
Q: What is the principle behind sterilizing media before use and before disposal
A: Microorganisms may be beneficial or harmful. The harmful microorganism causes many infections which…
Q: How is the CTSA (coconut water tryptic soy agar) growth medium designed to meet the nutritional need…
A: Many different kinds of media are formulated in the microbiology lab to support the growth of a wide…
Q: Why is Escherichia coli used as an indicator organism inmicrobial analyses of water?
A: The well-known gram-negative bacteria are E. coli (Escherichia coli).
Q: WHAT IS THE TYPE OF Growth patterns on Agar Slant and Broth Medium
A: Growth and morphogenesis of plant is determined by the composition of culture media. There are few…
Q: How is the generation time (g) of an exponentiallygrowing culture determined?
A: Bacteria are microorganism that most commonly occur in the soil, air, water and in adverse…
Q: Microbial Cell Density, Growth Curves Time (min) 0 20 40 60 80 100 Gp1 Abs 0.054 0.053 0.10 0.21…
A: The time taken by a population to double it's size is known as the doubling time. A semilog graph…
Q: Is the Mueller-Hinton Agar (MHA) a complex or defined medium? Explain based on its composition. Is…
A: Mueller and Hinton in 1941 developed Mueller Hinton Agar(MHA). MHA is used for routine…
Q: What is a complex medium? and What are the basic components of a culture medium?
A: Medium is defined as the solid or liquid substratum on which the cells or organ can grow for further…
Q: In determination of microbial motility, what is the value of a hanging-drop perparation? what is the…
A: Hanging drop is a motility testing which helps microbiologist to view motility of bacterium.
Q: What are the roles inhibitory substances and pH indicator in the selective and differential
A: Selective media is a specially designed to select the growth of a specific microorganism in a group…
Q: Of what practical importance are air borne microorganisms to the laboratory workers? What…
A:
Q: What are the criteria for media selection and the basic requirements for media formulation for…
A: Fermentation is a metabolic process that produces chemical changes in organic substrates through the…
Q: What is thermal death time? What are the factors that may influence the efficiency of chemical…
A: Answer 1 :- Thermal death time is the way lengthy it takes to kill a particular bacterium at a…
Q: Is nutrient agar (NA) a complex or defined medium? Explained based on its composition. Is NA a…
A: a complex media is a media in which the exact composition is not known . it usually conatins…
Q: What is the relationship between the most probable number method and nitrate in environmental…
A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…
Q: Why is egg enrichment not added to BPA prior to sterilization?
A: Baird–parker agar or BPA is a selective medium is identify or isolate S. aureus in food. It contains…
Q: ar contains salt to prevent the growth of most microbes and mannitol plus a pH indicator to detect…
A: In order to produce and culture different types of microbial species, a culture media is required.…
Q: Why do we grow the bacteria in minimal media when testing for citrate utilization
A: Minimal media are those that provide the minimal nutrients possible for colony development, usually…
Q: What are the factors that influence the efficiency of chemical sterilization?
A: Chemical Sterilization is the process of removal of microorganisms by the use of chemical…
Q: In simpler terms explain Lag, Log, Stationary, and Death Phase in the Microbial Growth Curve?
A: Bacterial growth: - Increase in the number of cells. - Bacterial growth in a closed system (Batch…
Q: what present microorganisms in flipper spoilage and springer spoilage
A: Spoilage of food is often observed in canned food items. There are roughly four types of bulged or…
Q: What are some characteristics of microbes and infectious agents that would require handling in a…
A: Since you have asked multiple question, we will solve the first question for you. If you want any…
Q: What are the advantages and disadvantages of plate count technique over other methods of quantifying…
A: Plate count is a technique in which bacteria are counted by growing on the agar plates. Total…
Microbial Physiology
Microbial physiology is the branch of microbiology that is associated with studying the physiology of fungi, bacteria, and viruses. It is an important field of science concerning functional genomics and metabolic engineering.
Chemotaxis
The organism’s movement in a particular direction as a response to certain chemicals that are available in the environment is defined as chemotaxis. In brief, it can be defined as the occurrence of an organism’s movement in response to a certain chemical stimulus.
Are you able to identify the lag, log, stationary and death phase in using this growth curve? Are you able to calculate the generation time for the microbial under this condition?
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