An integrated tool used for conducting automatic and manual sequence alignment of DNA and protein, and inferring phylogenetic relationships between identified sequences. NCBI: GenBank NCBI: Primer Blast Tool NCBI: Nucleotide Blast Tool Molecular Evolutionary Genetics Analysis (MEGA)
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- Objective: Get a sense of how genomics, the study of the genome in its entirety,needs to think about how to go about its research. Geonomic DNA is broken up into fragments. The 5’ and 3’ ends of each fragment(a “read”) are sequenced. The sequenced reads are assembled together intocontiguous sequences (“contigs”) based on sequence similarity. The idea is to sequence enough random fragments so that every nucleotide in thegenome is represented on some read. The number of such fragments needed iscalled the coverage, c. The coverage c can be calculated by the formula RL/G, where R is the number ofreads sequenced, L is the average length of a read and G is the total length of thegenome. The units of length are bases (b) or base pairs (bp). Consider a genome whose length is 1000 bp. “Shotgun” sequencing techniquesare applied to the genome, resulting in 20 reads, with an average length of 50 bp.A very important point is that, even though 20 x 50 = 1000, there is no guaranteethat ALL…Objective: Get a sense of how genomics, the study of the genome in its entirety,needs to think about how to go about its research. Geonomic DNA is broken up into fragments. The 5’ and 3’ ends of each fragment(a “read”) are sequenced. The sequenced reads are assembled together intocontiguous sequences (“contigs”) based on sequence similarity. The idea is to sequence enough random fragments so that every nucleotide in thegenome is represented on some read. The number of such fragments needed iscalled the coverage, c. The coverage c can be calculated by the formula RL/G, where R is the number ofreads sequenced, L is the average length of a read and G is the total length of thegenome. The units of length are bases (b) or base pairs (bp). Consider a genome whose length is 1000 bp. “Shotgun” sequencing techniquesare applied to the genome, resulting in 20 reads, with an average length of 50 bp.A very important point is that, even though 20 x 50 = 1000, there is no guaranteethat ALL…Which of the following is NOT an activity carried out in the field of bioinformatics? a. collecting and storing DNA sequence information produced by various genome sequencing projects b. analyzing genome sequences to determine the location of genes c. determining the three-dimensional structure of proteins d. comparing genomes of different species e. none of these
- DNA Profiles as Tools for Identification STRs are: a. used for DNA profiles b. repeated sequences present in the human genome c. highly variable in copy number d. all of these e. none of theseDNA extraction, PCR, gel electrophoresis, and DNA sequencing of PCR products. Give a description of these methods and the materials required to perform each of these molecular techniquesA genetic sequence database that contains annotated collection of all publicly available DNA sequences. NCBI: GenBank NCBI: Nucleotide Blast NCBI: Primer Blast Molecular Evolutionary Genetics Analysis (MEGA)
- Molecular biologists rely on many, often sophisticated, techniques to pursue their discipline. One may list ultracentrifugation, electron microscopy, X-ray diffraction, electrophoresis, and computer interfacing as fundamental tools. Model organisms provide the raw materials for study. List three "organisms" (or organismic groups) often used by recombinant DNA technologists and describe a major advantage of each group.A sunyocc.open.suny.edu/webapps/assessment/take/launch.jsp?course_assess * Question Completion Status: QUESTION 2 Correctly match the material/supply to its role (job) in genetic engineering. A. vector cuts gene and recipient DNA leaving sticky ends B. DNA ligase v moves newly combined DNA from a test tube into the organism C. restriction enzyme v living thing that gets a new gene and therefore a new trait D. host v glues the new gene and recipient DNA together QUESTION 3 Which of the following are PROS (good things) about genetic engineering? Choose ALL that apply. OA. better health or quality of life B. more products which means lower costs O C. animal welfare concerns O D. more toxins in the environment O E. safer method of sensing explosives OF. more herbicide in the environment O G. fewer crop varieties O H. cool pets QUESTION 4 Which of the following is FALSE about biotechnologies? Click Save and Submit to save and submit. Click Save All Answers to save all answers.DNA QUANTITATION Can you use the spectrophotometer to quantify proteins? Consult related references and list assays by which the spectrophotometer is used.
- Explain Shortly. I need help The emergence of new molecular biology techniques has allowed researchers to determine DNA sequences quickly and efficiently. A) How could knowledge of a DNA sequence be abused? B) How could knowing a DNA sequence be helpful? C) Would you ever consent to having your DNA sequenced. Explain your answerrRNA sequence analysis:a. What are two advantages of using sequence analysis of ribosomal components in determiningevolutionary relatedness of organisms? Please explain why each is an advantage.Computate bioinformatically the Tm value, the GC-content of the selected DNA sequence and the absolute nucleotide composition of the selected gene. Please provide also the tool source of the computation. Sickle Cell Anemia is the disease being used. Please answer the question using sickle cell anemia