Homework Help is Here – Start Your Trial Now!
Science
Biology
amp" gene -lacZ gene -Gene of interest Plasmid DNA - Unique restriction - Chromosomal DNA site Origin of- replication from human cells Cut the DNAS with the same restriction enzyme. Mix the DNAS together. Allow time for sticky ends to base-pair. Add DNA ligase to covalently link the DNA backbones. Gene of interest Another fragment of chromosomal DNA Recircularized Recombinant Recombinant or or vector vector vector Mix DNA with many E.coli cells that don't already have a plasmid. Treat the cells with agents that make them permeable to DNA. Note: This shows a bacterial cell that has taken up a plasmid carrying the gene of interest. Other bacterial cells would have other recombinant Recircularized vector without an insert; B-galactoside is made and converts X-Gal to a blue color. vectors or a recircularized vector. Plate cells on media containing X-Gal, IPTG, and ampicillin. Incubate overnight. Recombinant vector with an insert; Blue colony White colony B-galactosidase is inactive because of the inserted DNA. Each bacterial colony is derived from a single cell that has taken up a single plasmid. Therefore, all the cells in a colony are genetically identical. Cells that do not take up a plasmid cannot grow and form colonies because they are killed by ampicillin. FIGURE 21.2 The steps in gene cloning. Note: X-Gal refers to the colorless compound 5-bromo-4-chloro-3-indolyl- B-D-galactoside. It is converted by B-galactosidase into a blue dye. IPTG is an abbreviation for isopropyl-ß-D-thiogalactopyranoside, which is a nonmetabolizable lactose analog that induces the lac promoter. ONLUNE

amp" gene -lacZ gene -Gene of interest Plasmid DNA - Unique restriction - Chromosomal DNA site Origin of- replication from human cells Cut the DNAS with the same restriction enzyme. Mix the DNAS together. Allow time for sticky ends to base-pair. Add DNA ligase to covalently link the DNA backbones. Gene of interest Another fragment of chromosomal DNA Recircularized Recombinant Recombinant or or vector vector vector Mix DNA with many E.coli cells that don't already have a plasmid. Treat the cells with agents that make them permeable to DNA. Note: This shows a bacterial cell that has taken up a plasmid carrying the gene of interest. Other bacterial cells would have other recombinant Recircularized vector without an insert; B-galactoside is made and converts X-Gal to a blue color. vectors or a recircularized vector. Plate cells on media containing X-Gal, IPTG, and ampicillin. Incubate overnight. Recombinant vector with an insert; Blue colony White colony B-galactosidase is inactive because of the inserted DNA. Each bacterial colony is derived from a single cell that has taken up a single plasmid. Therefore, all the cells in a colony are genetically identical. Cells that do not take up a plasmid cannot grow and form colonies because they are killed by ampicillin. FIGURE 21.2 The steps in gene cloning. Note: X-Gal refers to the colorless compound 5-bromo-4-chloro-3-indolyl- B-D-galactoside. It is converted by B-galactosidase into a blue dye. IPTG is an abbreviation for isopropyl-ß-D-thiogalactopyranoside, which is a nonmetabolizable lactose analog that induces the lac promoter. ONLUNE

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question

Explain the role of the gene that is the selectable marker gene in this experiment.

amp" gene -lacZ gene -Gene of interest Plasmid DNA - Unique restriction - Chromosomal DNA site Origin of- replication from human cells Cut the DNAS with the same restriction enzyme. Mix the DNAS together. Allow time for sticky ends to base-pair. Add DNA ligase to covalently link the DNA backbones. Gene of interest Another fragment of chromosomal DNA Recircularized Recombinant Recombinant or or vector vector vector Mix DNA with many E.coli cells that don't already have a plasmid. Treat the cells with agents that make them permeable to DNA.
Transcribed Image Text:amp" gene -lacZ gene -Gene of interest Plasmid DNA - Unique restriction - Chromosomal DNA site Origin of- replication from human cells Cut the DNAS with the same restriction enzyme. Mix the DNAS together. Allow time for sticky ends to base-pair. Add DNA ligase to covalently link the DNA backbones. Gene of interest Another fragment of chromosomal DNA Recircularized Recombinant Recombinant or or vector vector vector Mix DNA with many E.coli cells that don't already have a plasmid. Treat the cells with agents that make them permeable to DNA.
Note: This shows a bacterial cell that has taken up a plasmid carrying the gene of interest. Other bacterial cells would have other recombinant Recircularized vector without an insert; B-galactoside is made and converts X-Gal to a blue color. vectors or a recircularized vector. Plate cells on media containing X-Gal, IPTG, and ampicillin. Incubate overnight. Recombinant vector with an insert; Blue colony White colony B-galactosidase is inactive because of the inserted DNA. Each bacterial colony is derived from a single cell that has taken up a single plasmid. Therefore, all the cells in a colony are genetically identical. Cells that do not take up a plasmid cannot grow and form colonies because they are killed by ampicillin. FIGURE 21.2 The steps in gene cloning. Note: X-Gal refers to the colorless compound 5-bromo-4-chloro-3-indolyl- B-D-galactoside. It is converted by B-galactosidase into a blue dye. IPTG is an abbreviation for isopropyl-ß-D-thiogalactopyranoside, which is a nonmetabolizable lactose analog that induces the lac promoter. ONLUNE
Transcribed Image Text:Note: This shows a bacterial cell that has taken up a plasmid carrying the gene of interest. Other bacterial cells would have other recombinant Recircularized vector without an insert; B-galactoside is made and converts X-Gal to a blue color. vectors or a recircularized vector. Plate cells on media containing X-Gal, IPTG, and ampicillin. Incubate overnight. Recombinant vector with an insert; Blue colony White colony B-galactosidase is inactive because of the inserted DNA. Each bacterial colony is derived from a single cell that has taken up a single plasmid. Therefore, all the cells in a colony are genetically identical. Cells that do not take up a plasmid cannot grow and form colonies because they are killed by ampicillin. FIGURE 21.2 The steps in gene cloning. Note: X-Gal refers to the colorless compound 5-bromo-4-chloro-3-indolyl- B-D-galactoside. It is converted by B-galactosidase into a blue dye. IPTG is an abbreviation for isopropyl-ß-D-thiogalactopyranoside, which is a nonmetabolizable lactose analog that induces the lac promoter. ONLUNE
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Genomic studies
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS