ACTIVITY NO.3 Given the data set below: a) Construct a frequency distribution table, b) show the Leaf and Stem, and c) Draw the Histogram and Polygon. 23, 34, 32, 21, 9. 35, 33, 22, 41, 40, 43, 8, 25, 46, 43, 50, 54, 52, 8, 11, 12, 13, 15, 20, 29
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- 13 Match each of the QPCR samples (i-v) with the correct amplification plot (A-E) and determine the Ct value for each of the amplification curves. Number of template copies in the QPCR reaction: Amplification plot (A-E): Ct Value 1200 1000 i. 1X 106 ii. 1X 105 iii. 1X 108 iv. 1X 107 V. NTC (No template control) 800 600 400 200 A C 0 5 10 15 20 25 30 Cycles vi. Create a standard curve graph of template copies vs Ct with the data in the table above. Log of Starting Quantity of DNA vrs C+ vii. 26 24 22221 20 18 16 14 12 כי 10 8 6 4 2 0 5 5.5 6 6.5 7 7.5 8 Log Starting Quantity of DNA (Template copies) If a patient sample has a Ct of 20, interpolate the number of template copies at the start of the PCR reaction using the standard curve you created above?The diagram shows a simplified cross-section of a human body and an ultrasound transducer.. 1swog meipsib eru of epnetster ritiW a) Explain what happens to the ultrasound signal as it travels from the probe through the body. b) Sketch a graph showing how the intensity of the signal detected by the probe varies with time following the transmission of a pulse of ultrasound with the probe in this positionB. subtilis ~1X3-4 micron • Notice: most cells appear purple while a few appear pink (arrow) Review fig 3.46 on page 171 to explain this phenomenon.
- (d) If one bacteria cell divides every 30 minutes, calculate the number of cells after (a) Assuming the bacterial cell divides every 20 minutes under standard conditions, (c) How many hours will it take to produce 8192 cells under the same conditions. If a bacterial culture with initial cells number (No) increases by a factor of 2 in one generation and n is the time as given by the formula in equation 1. N = (2")No -----(1) calculate the number of cell division in 4 hours. (b) Calculate the number of cells produced after 4 lhours. hours.Table 1a Why Are Cells Small Length (l) (cm) Width (w) (cm) Height (h) (cm) Size of Cross Section (h x w) (cm) Distance Traveled by IKI from Potato Edge (cm) Area of White Region (l x w) (cm) 2.50 2.50 2.50 6.25 cm 0.20 cm 2.64 cm 2.00 2.00 1.00 2.00cm 0.30 cm 1.87 cm 1.50 1.50 1.50 2.25 cm 0.50 cm 1.50 cm 1.00 1.00 1.00 1.00 cm 0.40 0.60 cm 2.00 0.50 0.50 0.25 cm 0.20 cm 0.15 cm 0.50 0.50 0.50 0.25 cm 0.30 cm 0.10 cm help to fill the table4) If 20% of a culture of human cells have a DNA content somewhere between 1&2xs (S-phase= 8 hours) and 1X amount of DNA in non-dividing cells, what is the generation time? A) 25 Hrs B) 30 hrs C) 0.025 hrs D) 40 hrs E) None of these
- Airborne Plate, matching 3 8. 6. 9 3 31. Determine the following for positive staining. (a) dye, (b) charge of the chromophore/colored ion, (c) charge of the target cellular structure, (d) applications/target cell structure 2.Determine the following for negative staining. (a) dye, (b) charge of the chromophore/colored ion, (c) charge of the target cellular structure, (d) applications/target cell structure10- 00 O SCNSA-SCN10A CDKN1A HANDI VTI1A SYTI • MYOCD 9 10 11 12 13 14 15 16 17 18 19 20 2122 X Y
- In what situation(s) would the use of a scanning electron microscope be ideal, and why?Imagine a sealed terrarium containing a plant and a beetle. How does each organism provide resources for the other? Could each organism survive if it was, the only living thing in the terrarium? Why or why not?With an A260 value of 1.12 and a DNA concentration of 56.0 ng/mL, what is the pathlength of the optical system used within the Nanodrop?