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a. Approximately how many amino acids are present in a 400 kDa protein. (show work) b. How many grams would there be in a nanomole of this protein? (show work) What is the purpose of adding DTT? d. How many subunits does the protein have, and what are their molecular weights?

a. Approximately how many amino acids are present in a 400 kDa protein. (show work) b. How many grams would there be in a nanomole of this protein? (show work) What is the purpose of adding DTT? d. How many subunits does the protein have, and what are their molecular weights?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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6. A protein has a molecular mass of 400 kDa when measured by size-exclusion chromatography. When subjected to gel electrophoresis in the presence of SDS the protein gives three bands with molecular m of 180, 160, and 60 kDa. When electrophoresis is carried out in the presence of SDS and dithiothreitol (DTT), three bands again form, this time with molecular masses of 160, 90, and 60 kDa. a. Approximately how many amino acids are present in a 400 kDa protein. (show work) b. How many grams would there be in a nanomole of this protein? (show work) C. What is the purpose of adding DTT? d. How many subunits does the protein have, and what are their molecular weights?
Transcribed Image Text:6. A protein has a molecular mass of 400 kDa when measured by size-exclusion chromatography. When subjected to gel electrophoresis in the presence of SDS the protein gives three bands with molecular m of 180, 160, and 60 kDa. When electrophoresis is carried out in the presence of SDS and dithiothreitol (DTT), three bands again form, this time with molecular masses of 160, 90, and 60 kDa. a. Approximately how many amino acids are present in a 400 kDa protein. (show work) b. How many grams would there be in a nanomole of this protein? (show work) C. What is the purpose of adding DTT? d. How many subunits does the protein have, and what are their molecular weights?
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