A temperature-sensitive mutant yeast strain stops dividing when shifted from 25°C to 37°C. Thesecells are analyzed at different temperatures by a machine that measures the amount of DNA theycontain, and the following graphs are obtained.number of cellsnumber of cells[ Select][Select][Select]25°C[Select]ملCells in G1 phase should have [Select]Which of the following would explain the behavior of your mutant? Mark yes or no for each of thefollowing choices.2amount of DNA/cell (arbitrary units)37°C12amount of DNA/cell (arbitrary units)unit(s) amount of DNA.✓ Inability to initiate DNA replicationDefect in chromosome condensation✓Defect in centrosome duplication✓Defect in cytokinesis Complete the following statements by selecting one of the two options provided.A mutation that [Select]arrest.The [Select]the kinase activity of S-Cdk will lead to cell-cycleof a cell cycle checkpoint will lead to cell-cycle arrest.A mutation that [Select]the activity of the monomeric GTPase Ras will leadto hyper-proliferation of cells [Hint: review Ras and the MAP kinase pathway covered in Topic 6].A mutation that [Select]proliferation of cells [Hint: review the MAP kinase pathway covered in Topic 6].the activity of MEK 1/2 will lead to hyper-

Answered: A temperature-sensitive mutant yeast…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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A temperature-sensitive mutant yeast strain stops dividing when shifted from 25°C to 37°C. These
cells are analyzed at different temperatures by a machine that measures the amount of DNA they
contain, and the following graphs are obtained.
number of cells
number of cells
[ Select]
[Select]
[Select]
25°C
[Select]
مل
Cells in G1 phase should have [Select]
Which of the following would explain the behavior of your mutant? Mark yes or no for each of the
following choices.
2
amount of DNA/cell (arbitrary units)
37°C
1
2
amount of DNA/cell (arbitrary units)
unit(s) amount of DNA.
✓ Inability to initiate DNA replication
Defect in chromosome condensation
✓Defect in centrosome duplication
✓Defect in cytokinesis

Complete the following statements by selecting one of the two options provided.
A mutation that [Select]
arrest.
The [Select]
the kinase activity of S-Cdk will lead to cell-cycle
of a cell cycle checkpoint will lead to cell-cycle arrest.
A mutation that [Select]
the activity of the monomeric GTPase Ras will lead
to hyper-proliferation of cells [Hint: review Ras and the MAP kinase pathway covered in Topic 6].
A mutation that [Select]
proliferation of cells [Hint: review the MAP kinase pathway covered in Topic 6].
the activity of MEK 1/2 will lead to hyper-

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The amount of DNA will be changed in a dividing cell and by observing the amount of DNA we can easily determine the cell is present in which phase of the cell cycle.

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