A 50 mL sample of soup was found to be contaminated with E. coli. A dilution of the soup was prepared by adding 14 mL of the soup to 20 mL of water. This was then diluted 1/60, and finally 10-3. Duplicate plates of the final dilution were prepared and after incubation, CFUs recorded as follows:                                                                                                                                           Volume plated (mL)               CFUs recorded                                            1.0                                     TNTC     305                                            0.25                                     72          65                                            0.1                                       27          29   Determine the concentration of E. coli in the original soup sample.

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Chapter2: Safety In Phlebotomy
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. A 50 mL sample of soup was found to be contaminated with E. coli. A dilution of the soup was prepared by adding 14 mL of the soup to 20 mL of water. This was then diluted 1/60, and finally 10-3. Duplicate plates of the final dilution were prepared and after incubation, CFUs recorded as follows:                                                                                                           

 

                             Volume plated (mL)               CFUs recorded

                                           1.0                                     TNTC     305

                                           0.25                                     72          65

                                           0.1                                       27          29

 

Determine the concentration of E. coli in the original soup sample.             

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