7. In the 1940s, Avery, MacLeod and McCarty, performed biochemical analyses of Griffith's "transforming" molecules from them. The only treatment that did not lead to the transformation of R to S strains was an principle. They prepared extracts from the pathogenic S, pneumoniae S strain and tested different extract to which they added the enzyme DNase, thus providing the first line of evidence that the genetic material is... Protein RNA DNA Cell wall carbohydrates A) B) m

7. In the 1940s, Avery, MacLeod and McCarty, performed biochemical analyses of Griffith's "transforming" molecules from them. The only treatment that did not lead to the transformation of R to S strains was an principle. They prepared extracts from the pathogenic S, pneumoniae S strain and tested different extract to which they added the enzyme DNase, thus providing the first line of evidence that the genetic material is... Protein RNA DNA Cell wall carbohydrates A) B) m

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter15: From Dna To Protein

Section15.1: The Connection Between Dna, Rna, And Protein

Problem 1SB

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Various antimicrobial drugs to treat microbial infection have diverse mechanism of action. Consider the following antimicrobial drugs: A. Seconeolitsine, known as DNA topoisomerase I inhibitor in bacteria. (i) Explain briefly how inhibiting DNA topoisomerase I is a good mechanism of action for an antibiotic, include possible molecular machineries being targeted. (ii) What would be an appropriate response if seconeolitsine works well by stating the state of supercoiling in bacteria. (iii) To prove your answer (ii), you test the condition of bacterial DNA by running gel electrophoresis, one has been treated with seconeolitsine (+ sample) and the other one is not (- sample). Explain the position of each + sample and – sample band on the gel in reference to the point of origin (where you load your samples) or how far each DNA sample travel across agarose gel. (iv) Explain why you would expect answer (iii) for each + sample and – sample. B.…
In a cotransformation experiment DNA was isolated from a donor strain that was proA+ and strC+ and sensitive to tetracycline. (The proA and strC genes confer the ability to synthesize proline and confer streptomycin resistance, respectively.) A recipient strain is proA− and strC− and isresistant to tetracycline. After transformation, the bacteria werefirst streaked on a medium containing proline, streptomycin, andtetracycline. Colonies were then restreaked on a medium containingstreptomycin and tetracycline. (Note: Each type of medium hadcarbon and nitrogen sources for growth.) The following resultswere obtained:70 colonies grew on the medium containing proline, streptomycin,and tetracycline, but only 2 of these 70 colonies grew whenrestreaked on the medium containing streptomycin and tetracyclinebut lacking proline.What would you expect the cotransformation frequency to be ifthe average size of the DNA fragments was 4 minutes and thetwo genes were 1.4 minutes apart?
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Could you explain how you would know if you successfully induced transformation? Thank you Did you successfully demonstrate transformation occurred? Why or why not? (How do I know, what do I look for?) Explain the control used in this experiment. What was it, and why was it necessary? Based on the calculation in the question above, comment on the relative difficulty of achieving successful transformation of E. coli. (Please explain the relative difficulty)
In the Avery, McLeod, McCarty Experiment where supernatant from heat killed, virulent S Strain pneumonia solutions were added to non-virulent R Strain pneumonia cell cultures and allowed to grow in liquid media (i.e., broth). In tubes where Protease was added to the supernatant prior to cell culture, what was the observed effect when plating and growing the S. pneumonia cells to solid media?
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