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5. Six colorectal cancer (CRC) patient samples and one normal colon biopsy from a healthy individual were analysed for Wnt signalling status in the laboratory. Three investigations were performed including a Western blot analysis of protein level (A), qRT-PCR analysis of MRNA level (B) and a measurement of luciferase level from a reporter assay (C). A Western blot В B-Catenin B-actin qRT-PCR 20- C-MYC C 15- Reporter assay 20- Axin2 50- 15- 40- O FOPFlash TOPFlash CRC patient #1 CRC patient #2 CRC patient #4 CRC patient #5 CRC patient #6 CRC patient #1 CRC patient #2 CRC patient #3 20- Survivin 15- 10- MAPK1 CRC patient #1 CRC patient #6 a) Which patients are exhibiting aberrant Wnt signalling? CRC patient #3 CRC patient #1 CRC patient #2 Normal Colon b) Explain your interpretation of the data and how you arrived at your conclusion. CRC patient #3 CRC patient #4 CRC patient #1 c) Describe some of the molecular events which could promote aberrant Wnt signalling in CRC patient #4 CRC patient #5 CRC patient #6 cancer. CRC patient #3 Normal colon CRC patient #1 CRC patient #2 CRC patient #3 CRC patient #4 CRC patient #5 CRC patient #6 Relative luciferase level Normal Colon CRC patient #2 Relative mRNA level CRC patient #4 Normal Colon CRC patient #5 CRC patient #3 Relative mRNA level Relative mRNA level Normal Colon CRC patient #5 CRC patient #6 CRC patient #4 CRC patient #5 CRC patient #6 Relative mRNA level Normal Colon CRC patient #2

5. Six colorectal cancer (CRC) patient samples and one normal colon biopsy from a healthy individual were analysed for Wnt signalling status in the laboratory. Three investigations were performed including a Western blot analysis of protein level (A), qRT-PCR analysis of MRNA level (B) and a measurement of luciferase level from a reporter assay (C). A Western blot В B-Catenin B-actin qRT-PCR 20- C-MYC C 15- Reporter assay 20- Axin2 50- 15- 40- O FOPFlash TOPFlash CRC patient #1 CRC patient #2 CRC patient #4 CRC patient #5 CRC patient #6 CRC patient #1 CRC patient #2 CRC patient #3 20- Survivin 15- 10- MAPK1 CRC patient #1 CRC patient #6 a) Which patients are exhibiting aberrant Wnt signalling? CRC patient #3 CRC patient #1 CRC patient #2 Normal Colon b) Explain your interpretation of the data and how you arrived at your conclusion. CRC patient #3 CRC patient #4 CRC patient #1 c) Describe some of the molecular events which could promote aberrant Wnt signalling in CRC patient #4 CRC patient #5 CRC patient #6 cancer. CRC patient #3 Normal colon CRC patient #1 CRC patient #2 CRC patient #3 CRC patient #4 CRC patient #5 CRC patient #6 Relative luciferase level Normal Colon CRC patient #2 Relative mRNA level CRC patient #4 Normal Colon CRC patient #5 CRC patient #3 Relative mRNA level Relative mRNA level Normal Colon CRC patient #5 CRC patient #6 CRC patient #4 CRC patient #5 CRC patient #6 Relative mRNA level Normal Colon CRC patient #2

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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5. Six colorectal cancer (CRC) patient samples and one normal colon biopsy from a healthy individual were analysed for Wnt signalling status in the laboratory. Three investigations were performed including a Western blot analysis of protein level (A), qRT-PCR analysis of MRNA level (B) and a measurement of luciferase level from a reporter assay (C). A Western blot B-Catenin В B-actin qRT-PCR 20- C-MYC 15- Reporter assay 50- 20- 40- Axin2 15- O FOPFlash CRC patient #1 CRC patient #2 TOPFlash Normal Colon CRC patient #5 CRC patient #6 CRC patient #3 20- Survivin Normal Colon CRC patient #1 CRC patient #2 15- CRC patient #4 CRC patient #5 CRC patient #6 МАРК1 Normal Colon 10- CRC patient #4° a) Which patients are exhibiting aberrant Wnt signalling? CRC patient #2 CRC patient #6 Normal Colon CRC patient #1 b) Explain your interpretation of the data and how you arrived at your conclusion. CRC patient #2 CRC patient #3 CRC patient #4 CRC patient #5 CRC patient #6 c) Describe some of the molecular events which could promote aberrant Wnt signalling in CRC patient #2 CRC patient #3 CRC patient #4 cancer. CRC patient #1 CRC patient #5 Normal colon CRC patient #1 CRC patient #2 CRC patient #3 CRC patient #4 CRC patient #5 CRC patient #6 Relative luciferase level CRC patient #11 CRC patient #3 Relative mRNA level CRC patient #5 CRC patient #4 Relative mRNA level Relative MRNA level CRC patient #3 Relative mRNA level Normal Colon CRC patient #6
Transcribed Image Text:5. Six colorectal cancer (CRC) patient samples and one normal colon biopsy from a healthy individual were analysed for Wnt signalling status in the laboratory. Three investigations were performed including a Western blot analysis of protein level (A), qRT-PCR analysis of MRNA level (B) and a measurement of luciferase level from a reporter assay (C). A Western blot B-Catenin В B-actin qRT-PCR 20- C-MYC 15- Reporter assay 50- 20- 40- Axin2 15- O FOPFlash CRC patient #1 CRC patient #2 TOPFlash Normal Colon CRC patient #5 CRC patient #6 CRC patient #3 20- Survivin Normal Colon CRC patient #1 CRC patient #2 15- CRC patient #4 CRC patient #5 CRC patient #6 МАРК1 Normal Colon 10- CRC patient #4° a) Which patients are exhibiting aberrant Wnt signalling? CRC patient #2 CRC patient #6 Normal Colon CRC patient #1 b) Explain your interpretation of the data and how you arrived at your conclusion. CRC patient #2 CRC patient #3 CRC patient #4 CRC patient #5 CRC patient #6 c) Describe some of the molecular events which could promote aberrant Wnt signalling in CRC patient #2 CRC patient #3 CRC patient #4 cancer. CRC patient #1 CRC patient #5 Normal colon CRC patient #1 CRC patient #2 CRC patient #3 CRC patient #4 CRC patient #5 CRC patient #6 Relative luciferase level CRC patient #11 CRC patient #3 Relative mRNA level CRC patient #5 CRC patient #4 Relative mRNA level Relative MRNA level CRC patient #3 Relative mRNA level Normal Colon CRC patient #6
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