5. If a virus has a negative-sense RNA genome, what enzymatic activity (if any) will be found as part of the virion structure and what will be the first step in expression of the viral genome? 6. Influenza viruses gain entry into their host cells by attachment to N-acetylneuraminic acid residues on the cell surface, followed by receptor-mediated endocytosis. Predict what effect the treatments shown in the table below will have on (i) the attachment of an influenza virus to a susceptible host cell, and (ii) the subsequent uncoating of influenza virus in the same cell. Use a “+" to indicate that the event will take place or a “–" to indicate that it will not take place. Note: In each case it is assumed that the events would be occurring in the same cell that has undergone the treatment. Treatment Attachment? Uncoating? Treatment of the host cell with neuraminidase Treatment of the host cell with NH,Cl, which prevents lowering of the lysosomal pH Treatment of the host cell with actinomycin D, which prevents synthesis of mRNA
5. If a virus has a negative-sense RNA genome, what enzymatic activity (if any) will be found as part of the virion structure and what will be the first step in expression of the viral genome? 6. Influenza viruses gain entry into their host cells by attachment to N-acetylneuraminic acid residues on the cell surface, followed by receptor-mediated endocytosis. Predict what effect the treatments shown in the table below will have on (i) the attachment of an influenza virus to a susceptible host cell, and (ii) the subsequent uncoating of influenza virus in the same cell. Use a “+" to indicate that the event will take place or a “–" to indicate that it will not take place. Note: In each case it is assumed that the events would be occurring in the same cell that has undergone the treatment. Treatment Attachment? Uncoating? Treatment of the host cell with neuraminidase Treatment of the host cell with NH,Cl, which prevents lowering of the lysosomal pH Treatment of the host cell with actinomycin D, which prevents synthesis of mRNA
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 4.2P
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