5. Construct a gene map given the following informa Which two genes are most likely going to be inherite together? Genes Crossover Frequency А & C 8% A & D 10% B & D 14% В & F 8% C & D 2% C & F 8% D & F 6%
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- 4e. You also study the expression of 3 different mutants for this gene. For each mutant answer the following: Does this mutation change the sequence of the protein produced? Why or why not? If it does change the sequence of protein be sure to write out the new sequence. If it does not change the protein sequence, what effect (if any) would you expect it to have on expression of the gene? 1 20 ORI 40 60 5'...TTCGAGCTCTCGTCGTCGAGATACGCGATGATATTACTGGTAATATGGGGATGCACTATC...3' 3' ...AAGCTCGAGAGCAGCAGCTCTATGCGCTACTATAATGACCATTATACCCCTACGTGATAG...5' * promoterAnswer the following questions. 1. Construct a map for the genes d,e,f. Assume that: d and e = 3%; e and f = 5%. Give 2 arrangements of the genes/maps. 2. If d and f = 2%, what is the correct arrangement of the genes d,e,f? 3. Consider the fourth gene "g". if g and e = 1.5%, give two possible arrangements. 4. If d and g = 1.5 % give the correct order of the four genes %3DThe photos above show flowers from Arabidopsis plants. One plant is wild-type (unmutated); the other carries a mutation in one of its ABC floral identity genes. This mutation causes sepals and petals to form instead of stamens and carpels. Refer to Figure 10.8 to decide which gene (A, B, or C) has been inactivated by the mutation.
- Discuss Concepts Many human diseases are caused by recessive alleles that are not expressed in heterozygotes. Some people think that eugenicsthe selective breeding of humans to eliminate undesirable genetic traitsprovides a way for us to rid our populations of such harmful alleles. Explain why eugenics cannot eliminate such genetic traits from human populations.6. Suppose a particular gene is required for early development and also later for development of a particulartissue, such as the adult nervous system. By generating a homozygous mutant clone in that tissue of a heterozygote, researchers can circumvent the lethalitythat would result if the entire animal is homozygousfor a loss-of-function mutation in that gene.A technique called MARCM (Mosaic Analysiswith a Repressible Cell Marker) was developed to enable Drosophila geneticists to generate homozygousmutant cell clones that are marked by the presence of areporter protein such as GFP. Marker expression enables the investigator to observe clearly the mutantphenotype within a clone of mutant cells. This technique relies on a yeast protein called Gal80 that is anegative regulator of the Gal4 protein described previously in Solved Problem II. Gal80 binds to Gal4 andprevents it from activating transcription. The idea ofMARCM is that Gal4/UASG-driven GFP expression isblocked by Gal80 throughout…8. Below is a diagram of the MYC gene, match the arrows with the correct description. 127.73M Genes (Comprehensive set... MYC-201 protein coding - MYC-209 protein coding DO MYC-203> protein coding 0- protein coding 2 - MYC-208> protein coding O MYC-202 protein coding O MYC-205> protein coding MYC-207> protein coding D 0 MYC-204 processed transcript 1 MYC-211> protein coding ← MYC-206> protein coding 2 i. Arrow 1 is pointing at ii. Arrow 2 is pointing at A. polyadenylation site B. 3' untranslated region C. transcription start site D. intron -27-52 kb 127.74M - MYC-212 > arotein codina E. 5' untranslated region F. promoter region G. transcription stop site H. protein coding region 127.73
- 1. Construct a map for the genes d,e,f. Assume that: d and e = 3%; e and f = 5%. Give 2 arrangements of the genes/maps.1. A monogenic disease is a disease caused by a mutation in a single gene. For instance, sickle-cell anemia is caused by a mutation in the HBB gene, which codes for the B- globin chain of hemoglobin. The beginning of HBB is shown here: 5'-ATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACT...-3' A. Translate this HBB sequence into an amino acid sequence. B. In terms of amino acids, what is the result of the sickle cell mutation, wherein the bolded red A is changed to a T? This single mutation causes hemoglobin to aggregate, causing red blood cells to deform into a sickle-like shape rather than the normal “biconcave disk" shape. C. What would happen if the bolded blue A were mutated to at T? (This is hypothetical; it's not a mutation found in sickle-cell disease.)a. What is your epigenome (i.e. epigenetics)? b. Does lifestyle affect your epigenome? Explain -c. Does your epigenome change with age? Explain d. What is epigenetic therapy? Is it working? Explain Edit View Insert Format Tools Table 12pt v Paragraph v в I Address DELL F7 F8 F9 F10 F1 F4 F5 F6 # % & 3 4 6. 7 8 9 Y ...
- 1. The first genetic test used a short DNA sequence that was closely linked to the Huntington’s locus. The linkage distance between the DNA sequence used in the test and the actual Huntington’ disease locus was 5%. a. In a large sample of the Venezuelan family (over 2,000 individuals), what percentage of people would inherit the DNA sequence but not the Huntington’s allele (i.e. be a false positive test for Huntington’s)? b. What percentage of that large Venezuelan family would not inherit the DNA sequence but would inherit the Huntington’s allele (i.e., be a false negative test for Huntington’s)?4. The A and 1B alleles in human blood are co-dominant but both are completely dominant to the i (give O blood type) allele. Fill in the table: Phenotype Genotype(s) A blood OR B blood OR AB blood poojq o Problem: Can a man who has O blood father a child with B blood? Explain your answer. Personet use only, do mOeproduce cd.eou garciato187 5 Hemophilia is a sex-linked recessive gene found on the X chromosome. A woman who is heterozygous for the gene has children with a man who has the condition. a What is the genotype of the man? What is the genotype of the woman? b Gametes produced by the man? Gametes produced by the woman? C In the space provided, draw a Punnett square for the parents previously described in this question. Include phenotype in each box. Next to your Punnett square, clearly identify the ratios for both genotype and phenotype. Personalise ony cootreoroduIce 70 TT-0207 GAICiato187@student.laccd.edu Personal use oniv do not reproduce 20-TT-0707 garciafo187@student…2. a. You want to create a genetic construct that will express GFP in Drosophila. In addition to the GFPcoding sequence, what DNA element(s) must youinclude in order to express this protein in flies if theconstruct were integrated into the Drosophila genome? Where should such DNA element(s) be located? How would you ensure that GFP is expressedonly in certain tissues of the fly, such as the wing?b. Suppose you insert the GFP coding region plus allof the DNA elements required by the answer to part(a)—except the enhancer—between inverted repeatsfound at the ends of a particular transposable element.Because all of the DNA sequences located betweenthese inverted repeats can move from place to placein the Drosophila genome, you can generate manydifferent fly strains, each with the construct integrated at a different genomic location. You now examine animals from each strain for GFPfluorescence. Animals from different strains showdifferent patterns: some glow green in the eyes,others in…