4. You have been given 6 new strains of Bacillus species recently isolated from a mining site. In order to compare the growth patterns of these strains, you set up a 24 hour growth experiment. The bacteria were grown at 37°C in a nutrient broth medium and samples were taken after 24 hours. Each sample was then serially diluted from 10.¹ to 104, and 100 μl aliquots from each plated onto nutrient agar plates for enumeration (using the spread plate method). The plates were incubated overnight and the colony counts recorded in the table below. Strain number B1 B2 B3 B4 B5 B6 10-¹ TMTC 935 TMTC TMTC 156 570 B1 B2 B3 B4 B5 10-² B6 TMTC 99 391 TMTC 21 67 Dilution 10-³ 786 25 48 670 4 12 10-4 220 Note: TMTC = Too Many To Count Complete the table below by calculating the number of CFU/ml for each of strains listed above Strain number CFU/ml 0 4 112 0 0

4. You have been given 6 new strains of Bacillus species recently isolated from a mining site. In order to compare the growth patterns of these strains, you set up a 24 hour growth experiment. The bacteria were grown at 37°C in a nutrient broth medium and samples were taken after 24 hours. Each sample was then serially diluted from 10.¹ to 104, and 100 μl aliquots from each plated onto nutrient agar plates for enumeration (using the spread plate method). The plates were incubated overnight and the colony counts recorded in the table below. Strain number B1 B2 B3 B4 B5 B6 10-¹ TMTC 935 TMTC TMTC 156 570 B1 B2 B3 B4 B5 10-² B6 TMTC 99 391 TMTC 21 67 Dilution 10-³ 786 25 48 670 4 12 10-4 220 Note: TMTC = Too Many To Count Complete the table below by calculating the number of CFU/ml for each of strains listed above Strain number CFU/ml 0 4 112 0 0

Comprehensive Medical Assisting: Administrative and Clinical Competencies (MindTap Course List)

6th Edition

ISBN:9781305964792

Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Chapter42: Basic Microbiology

Section: Chapter Questions

Problem 4CR

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1. What is one advantage of utilizing the pour plate technique over the streak plate technique ? 2. Why must the agar pours be cooled to 45C before use in the pour plate technique? 3. Explain the consequences if a group removed all the agar pours from the water bath at one time and allowed them to sit on the bench for several minutes before using them. 4. Why can the agar pour tubes be rinsed in the sink after the agar is transferred to the Petri plate ? Could you rinse the tubes if the bacteria had been pipetted into the agar pour tubes rather than in the plates? Explain. 5. What would be the result if a student dipped his / her loop in the stock culture during inoculations of each quadrant ? Explain . part B 1. The introduction stated that microbes are mechanically separated or diluted over the surface of the medium . How is this accomplished ? 2. Go to https://commons.wikimedia.org/wiki/File:MacConkey_agar_with_LF_and_LF_colonies . . Which side of the plate (left or right)…
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