3. Compute for the amount of each component of KCN broth if you were to prepare 280 ml. Express your answers using two decimal places. Follow this format in typing your answers: answerabbreviated unit (e.g. 3.00 g, 0.75 ml, 10.25 mg):
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- 3. Compute for the amount of each component of KCN broth if you were to prepare 280 ml. Express your answers using two decimal places. Follow this format in typing your answers: answerabbreviated unit (e.g. 3.00 g, 0.75 ml, 10.25 mg): Components Amount per liter Polypeptone 3.0 g N22HPO4 5.64 g (Available as NazHPO4 · 7H2O*) Monopotassium phosphate 0.225 g Sodium chloride 0.5% KCN 0.075 g (Available as 10,000 ppm solution) *MW (g/mol): Na-23; P-31; H-1; 0-16 3.3. Monopotassium phosphate /r3. Compute for the amount of each component of KCN broth if you were to prepare 280 ml. Express your answers using two decimal places. Follow this format in typing your answers: answerabbreviated unit (e.g. 3.00 g, 0.75 ml, 10.25 mg): Components Amount per liter Polypeptone 3.0 g NazHPO4 5.64 g (Available as NazHPO4 · 7H2O*) Monopotassium phosphate 0.225 g Sodium chloride 0.5% KCN 0.075 g (Available as 10,000 ppm solution) *MW (g/mol): Na-23; P-31; H-1; O-16 3.4. Sodium chloride (3. Compute for the amount of each component of KCN broth if you were to prepare 280 ml. Express your answers using two decimal places. Follow this format in typing your answers: answerabbreviated unit (e.g. 3.00 g, 0.75 ml, 10.25 mg): Components Amount per liter Polypeptone 3.0 g NazHPO4 5.64 g (Available as NazHPO4 · 7H2O*) Monopotassium phosphate 0.225 g Sodium chloride 0.5% KCN 0.075 g (Available as 10,000 ppm solution) *MW (g/mol): Na-23; P-31; H-1; 0-16 3.2. Na2HPO4 • 7H2O!'
- Calculate the unknown concentration of the PROTEIN C with an absorbance value of A412 given the standard curve indicated in the table below. Write your final answer (NUMBER ONLY) in two decimal places rounded off. Protein concentration (µg/mL) 0 0.02 0.04 0.06 0.08 0.10 Your answer APIENT Absorbance 0.000 0.161 0.284 0.438 0.572 0.762i) Write down the equation derived from your Excel generated standard curve , figure legend and describe its components; ii) provide the values of the absorbance data of the unknown sample (do NOT show the absorbance data of the glycine standards). Show all details of the working out of your calculation. Indicate all units! Provide the answer with two decimals precisionData for standard glucose concentrations Glucose concentration (mm) 0 0.5 1 1.5 -~~ 2 2.5 A Data for plasma samples B Patient Timepoint (hours) C D E 0 0.5 1 2 0 0.5 1 2 0 0.5 1 2 1. Draw a standard curve of absorbance at 420nm against glucose concentration in mmoles/L. We recommend you use Excel or a similar programme to insert the graph Alternatively, you can draw by hand on graph paper and insert a photo of the graph. OON 0 0.5 1 Absorbance 1 0.000 0.477 0.947 1.234 1.733 2.160 2 0 0.5 1 Absorbance Absorbance 2 0.000 0.482 0.941 1.236 1.721 2.276 Absorbance Glucose concentration Glucose concentration Abs 1 Abs 2 Average from graph (mM) in plasma (mm) 0.635 0.632 0.629 1.368 1.370 1.369 1.382 1.388 1.385 1.151 1.147 1.149 0.566 0.844 0.869 0.772 0.395 0.397 0.399 0.850 0.858 0.854 0.738 0.744 0.741 0.539 0.531 0.535 0.776 0.780 0.778 1.475 1.481 1.478 1.446 1.444 1.445 1.302 1.296 1.299 0.560 0.563 0.848 0.846 0.871 0.870 0.774 0.773 Average 0.000 0.480 0.944 1.235 1.727 2.218 0.440…
- a) The total amount excreted into urine as unchanged drug after an oral tablet (A) of 50 mg was 36.4 mg. After an oral capsule dose (B) of 25 mg the Uo was 20.3 mg. Calculate the relative bioavailability of the tablet A with respect to capsule B. If the absolute bioavailability of the oral capsule is determined to be 0.86 calculate the absolute bioavailability of the tablet dosage form. You are required to list the given data, formula and steps for calculationsi) Write down the equation derived from your Excel generated standard curve and describe its components; ii) provide the values of the absorbance data of the unknown sample (do NOT show the absorbance data of the glycine standards). Show all details of the working out of your calculation. Indicate all units! Provide the answer with two decimals precision iii) state the answer in a full sentence. (note: avoid mathematical symbols [=, +, -, etc] in your answer sentence).5. Using this formula Cu = Au X Cstd. Astd Where: C = concentration A = absorbance U = unknown/sample STD = standard Compute for the glucose concentration of the patient's sample. Absorbance of the standard = 0.075; Concentration of the standard = 10.54 Absorbance = 0.83 Convert you answer to mmol/L or S.I. using the conversion factor for glucose which is 0.055 Please show your computation.
- Ibuprofen for infusion comes in vials containing 400 mg of ibuprofen in 4 mL. Scotty requires 410 mg of ibuprofen to be infused every 6 hours. The solution for infusion has a concentration of 2.5 mg per mL. Calculate the volume of 5% glucose solution required to dilute the concentrated solution. Answer: 0.44 mL X *********What is the amount of dextrose in mg/kg/min provided by the following TPN for a 60 kg patient? Round to 1 decimal place, do not enter units Dextrose (70%) 320 g Amino acids (8.5%) 92 g Lipids (10%) 44 g sodium chloride 60 mEq sodium acetate 40 mEq Sodium phosphate 15 mmol potassium chloride 80 mEq potassium acetate 30 mEq calcium gluconate 18 mEq magnesium sulfate 8 mEq Multivitamins 10 mL Trace elements 1 mL Total volume: 2640 mL Rate: 110 mL/hrGeneral Blood Urine BP: 95/58 pH: 7.1 Odor: "fruity acetone" HR: 110 bpm Glucose: 345 mg/dl pH: 4.3 Ketone bodies: 22 mg/dl Glucose: Strongly positive Blood alcohol: 110 mg/dl 4. Explain how the pH of Mr. Bouchard's blood and urine is related to the ketone bodies measured in each of these fluids. 5. Provide a possible "alternative" explanation for Mr. Bouchard's erratic behavior and high blood alcohol concentration. Think outside the box. 6. What metabolic disease do you suspect Mr. Bouchard suffers from?