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- V e. kidney stones Which of the following microorganisms require the major available (aW) of water to proliferate? ¿Cuál de los siguientes microorganismos requiere la mayor cantidad de agua disponible (aW) para proliferar? O Penicillium spp. O Xeromyces spp. O Escherichia coli O Pseudomonas aeruginosa O Staphylococcus aureus Mention and describe the two types of bacterial resistance to antibiotics. Mencione y describa los dos tipos de resistencia bacteriana a los antibióticos. A-Which can infect hur Table 1: Absorbance and appearance of broth tubes at various temperatures. E. colin B. stearothermophilus Temperature°C Absorbance Visual Absorbance Visual 4 0.04 Clear 0.06 Clear tre Light turbid Very turbid Almost clear 25 0.66 0.09 Clear Light turbid Very turbid 37 1.48 0.55 which 55 0.25 1.29 oledzethe mes he alhe ive to edeut et brahell bras atnstooinieib o ho leviviue zwolls ti saunSuppose you spilled two cultures of Salmonella typhimur-ium (each containing 100,000 cells) on your lab bench. You im-mediately applied the same disinfectant to both cultures at thesame time. One culture had been freshly grown for 36 hours andthe other culture is 2 weeks old. If the kill ing rate of the disin-fectant is 90% per minute, do you think that microbes in bothcultures will be completely killed after 6 minutes? Why or whynot?
- 1. Explain the regulation of microbial growth by turgor pressure.For each item on the following list, propose a reasonable method ofsterilization. You cannot use the same method more than three timesfor the whole list. The method must sterilize, not just disinfect; and themethod must not destroy the item or render it useless unless there is noother choice. After considering a workable method, think of a methodthat would not work. Note: Where an object containing something isgiven, you must sterilize everything (for example, both the jar and thepetroleum jelly [Vaseline] in it). Some examples of sterilizing methodsare autoclave, ethylene oxide gas, dry oven, and ionizing radiation.room airseruma pot of soilplastic Petri dishesheat-sensitive drugscloth dressingsleather shoes from a thrift shopa cheese sandwicha flask of nutrient agaran entire room (walls, floor,etc.) rubber glovesdisposable syringescarcasses of cows with “madcow” diseaseinside of a refrigeratorwinea jar of Vaselinefruit in plastic bagsmilkorchid seedsmetal instrumentsmail contaminated…1 H 2 176 old culture Smear too BOTERC Losk like? + b. Failure to apply the decolorizer. + ne c. Failure to apply the safranin. Determine whether bacteria are motle or non-motle by looking at the growth in semi-solid medium What is the concentration of agar in (a) typical solid medium (b) semi sold motility medium? Arawer Q1 on Page 357. 7. Orygen Requirement: + d. Reversal of crystal violet and safranin stains. A questions 1.2 and 3 o Pg 83-84 Refer to the power point Questions 1 Predict the effect of the following "mistakes" made when performing a Gram stain. Your answer needs to address the predicted effect on both Gram-positive and Gram-negative cells. Consider each mistake independently. a. Failure to add the iodine. 414 gait to fer. 2 Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram-positive and Gram-negative cells. This being the case, explain how they stain different cell types in the Gram stain. why? a cell wall. patem in…
- Spores and toxin-producing microorganism produce toxins need higher Aw than minimum Aw for microbial growth. How could food industry benefit from this fact?9. Is the eosin methylene blue agar (EMB) a complex or defined medium? Explain based on its composition. Is MSA a A) differential, B) selective, or C) both differential and selective media? Explain based on what kind of microorganisms it allows to grow.35. .is the optimum condition for bacterial growth in soil? .... O Alkali O Acidic O Slightly acidic to neutral O Neutral to slightly alkaline The ATHER CENUINE
- A PhD student leaving for vacation has asked an undergraduate student to perform daily media changes forhis iPSCs while he is gone. The culture is happening in 12-well plates where a volume of 2 mL is optimal. OnSaturday, when changing the media, the undergraduate decides to add 4 ml of media to the dishes (insteadof 2 ml) because he wants to skip lab and watch the Super Bowl on Sunday. He decides to add twice thevolume of media (4 mL) to tide the cells over till Monday. However, when the graduate student returns onMonday, he finds that some of his cells have died. Your job is to determine whether the cells died due to alack of oxygen. For the calculations that follow, diffusion and reaction occurs only in one direction. Also,assume that reaction only occurs at the cell-media interface. Use Michaelis-Menten type kinetics for oxygenuptake. You may use the following information:Ps = 150 mmHg (ambient oxygen tension)K = 1.19 nmol / mL / mmHg (solubility of oxygen in medium)D = 2…A PhD student leaving for vacation has asked an undergraduate student to perform daily media changes forhis iPSCs while he is gone. The culture is happening in 12-well plates where a volume of 2 mL is optimal. OnSaturday, when changing the media, the undergraduate decides to add 4 ml of media to the dishes (insteadof 2 ml) because he wants to skip lab and watch the Super Bowl on Sunday. He decides to add twice thevolume of media (4 mL) to tide the cells over till Monday. However, when the graduate student returns onMonday, he finds that some of his cells have died. Your job is to determine whether the cells died due to alack of oxygen. For the calculations that follow, diffusion and reaction occurs only in one direction. Also,assume that reaction only occurs at the cell-media interface. Use Michaelis-Menten type kinetics for oxygenuptake. You may use the following information:Ps = 150 mmHg (ambient oxygen tension)K = 1.19 nmol / mL / mmHg (solubility of oxygen in medium)D = 2…A PhD student leaving for vacation has asked an undergraduate student to perform daily media changes forhis iPSCs while he is gone. The culture is happening in 12-well plates where a volume of 2 mL is optimal. OnSaturday, when changing the media, the undergraduate decides to add 4 ml of media to the dishes (insteadof 2 ml) because he wants to skip lab and watch the Super Bowl on Sunday. He decides to add twice thevolume of media (4 mL) to tide the cells over till Monday. However, when the graduate student returns onMonday, he finds that some of his cells have died. Your job is to determine whether the cells died due to alack of oxygen. For the calculations that follow, diffusion and reaction occurs only in one direction. Also,assume that reaction only occurs at the cell-media interface. Use Michaelis-Menten type kinetics for oxygenuptake. You may use the following information:Ps = 150 mmHg (ambient oxygen tension)K = 1.19 nmol / mL / mmHg (solubility of oxygen in medium)D = 2…