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250 150 2 -250 150 100 -100 75 -75 50 -50 37- 37 25 20 15 10 Std Calf Fibrinogen BSA Std Biorad Biorad 161-0374 serum Transferrin 161-0318 -25 -20 -15 -10 2. Measure the distance (in mm) moved for each protein standard band and record in Table3.6 below. Table 3.6 Example of table for recording of data-measured distance moved and calculated Rr values Item Band Distance moved Rr Log 10 Mr (from Calculated Mr (antilog (mm) graph) of the previous column) Sample C Band 1 Sample F Band 1 Band 2 Sample B Band 1 Band 2 Sample T Band 1

250 150 2 -250 150 100 -100 75 -75 50 -50 37- 37 25 20 15 10 Std Calf Fibrinogen BSA Std Biorad Biorad 161-0374 serum Transferrin 161-0318 -25 -20 -15 -10 2. Measure the distance (in mm) moved for each protein standard band and record in Table3.6 below. Table 3.6 Example of table for recording of data-measured distance moved and calculated Rr values Item Band Distance moved Rr Log 10 Mr (from Calculated Mr (antilog (mm) graph) of the previous column) Sample C Band 1 Sample F Band 1 Band 2 Sample B Band 1 Band 2 Sample T Band 1

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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250- 1 2 3 4 5 6 -250 150- -150 100- -100 75- -75 50- 37- 25- 20- 15- 10 10- Std Biorad BSA Calf serum Fibrinogen Std Biorad Transferrin 161-0318 161-0374 -50 -37 -25 -20 15 10 2. Measure the distance (in mm) moved for each protein standard band and record in Table3.6 below. Table 3.6 Example of table for recording of data - measured distance moved and calculated Rr values Item Band Distance moved (mm) Rf Log 10 Mr (from graph) Calculated Mr (antilog of the previous column) Sample C Band 1 Sample F Band 1 Band 2 Sample B Band 1 Band 2 Sample T Band 1
Transcribed Image Text:250- 1 2 3 4 5 6 -250 150- -150 100- -100 75- -75 50- 37- 25- 20- 15- 10 10- Std Biorad BSA Calf serum Fibrinogen Std Biorad Transferrin 161-0318 161-0374 -50 -37 -25 -20 15 10 2. Measure the distance (in mm) moved for each protein standard band and record in Table3.6 below. Table 3.6 Example of table for recording of data - measured distance moved and calculated Rr values Item Band Distance moved (mm) Rf Log 10 Mr (from graph) Calculated Mr (antilog of the previous column) Sample C Band 1 Sample F Band 1 Band 2 Sample B Band 1 Band 2 Sample T Band 1
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