2. Gey used "witches' brews" for cell culture media. Researchers using HeLa cells now used a specially-formulated media (formulation linked below) with fetal bovine serum (serum from the blood of calves) to grow the cells. Despite being a strange mix of components, the "witches' brews" worked. Explain why by comparing the "witches' brews" to the components found in the media used today.

2. Gey used "witches' brews" for cell culture media. Researchers using HeLa cells now used a specially-formulated media (formulation linked below) with fetal bovine serum (serum from the blood of calves) to grow the cells. Despite being a strange mix of components, the "witches' brews" worked. Explain why by comparing the "witches' brews" to the components found in the media used today.

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter16: Adult And Pediatric Dosages Based On Body Surface Area

Section: Chapter Questions

Problem 7.2P

See similar textbooks

Similar questions

13. Compare the cells in the two photomicrographs below in terms of their shape and structure(s). А B
1. What substance is often applied on a slide specimen of cells or tissues that help enhance the appearance of cell parts under the microscope? 2. Name a very common, easily available, solution that is applied on onion cells or tissue to enhance their microscopic appearances?(
6. Consider an experiment in which cells are radioactively labeled by this method for only a short period of time (about 30 minutes). The radioactive thymidine medium is then replaced with medium containing unlabeled thymidine, and the cells are grown for some additional time. At different time points after replacement of the medium, cells are examined in a microscope. The fraction of cells in mitosis (which can be easily recognized because their chromosomes are condensed) that have radioactive DNA in their nuclei is then determined and plotted as a function of time after the labeling with radioactive thymidine. labeled mitotic cells (%) 100 75- 50 25 0 bod 0 5 10 15 20 25 30 time (hours) a) Would cells at all phases of the cell cycle contain radioactive DNA immediately after the labeling procedure? Why? b) Why are there initially no mitotic cells that contain radioactive DNA? c) Explain the rise and fall and then rise again of the curve. d) Estimate the length of G2 for these cells e)…
Determine the effect of 10% salt solution as a nasal spray to relieve congestion in infants with stuffy noses, on the cells lining the nasal cavity as prescribed by the pediatrician.
2. Consider the following paragraph from a peer-reviewed publication detailing the extraction and isolation of a lectin: "30 g of dry S. sclarea seeds were ground in a Waring blender and the meal was stirred overnight in 400 ml of Phosphate Buffered Saline (PBS), at 4 °C. The solution was centrifuged at 20,000 X g for 30 min, and the pellet was re-extracted by mixing with another 400 ml of PBS, overnight at 4°C. The two supernatants were combined and frozen at -20 °C. After thawing, the insoluble material was spun down (3,500 X g for 30 min), and the clear supernatant (crude extract) was precipitated with 50% (v/v) cold ethanol at 4 °C. After centrifugation (20,000 X g for 30 min), the pellet was discarded, and the supernatant was further precipitated with cold ethanol up to 80% (v/v) at 4 °C. After one night in the cold room, the solution was centrifuged (20,000 X g for 30 min), the supernatant was discarded, and the pellet was re-dissolved in water, dialyzed for 3 days at 4 °C…
2. Consider the following paragraph from a peer-reviewed publication detailing the extraction and isolation of a lectin: "30 g of dry S. sclarea seeds were ground in a Waring blender and the meal was stirred overnight in 400 ml of Phosphate Buffered Saline (PBS), at 4 °C. The solution was centrifuged at 20,000 X g for 30 min, and the pellet was re-extracted by mixing with another 400 ml of PBS, overnight at 4°C. The two supernatants were combined and frozen at -20 °C. After thawing, the insoluble material was spun down (3,500 X g for 30 min), and the clear supernatant (crude extract) was precipitated with 50% (v/v) cold ethanol at 4 °C. After centrifugation (20,000 X g for 30 min), the pellet was discarded, and the supernatant was further precipitated with cold ethanol up to 80% (v/v) at 4 °C. After one night in the cold room, the solution was centrifuged (20,000 X g for 30 min), the supernatant was discarded, and the pellet was re-dissolved in water, dialyzed for 3 days at 4 °C…
4. Here is an immunoassay to measure the explosive trinitrotoluene (TNT) in soil. An organic solvent is used to extract TNT from the soil and then the solvent is analyzed using a flow cytometer. A flow cytometer is a device used to count small particles that flow through a narrow tube past a detector. The particles are irradiated with a green laser and the fluorescence from each particle is measured as it flows past the detector. Step 1. Antibodies that bind TNT are chemically attached to 5-μm latex beads. Step 2. The beads are incubated with fluorescent derivative of TNT to saturate the antibodies and excess TNT is washed away. The beads are suspended in aqueous detergent. Step 3. A volume of 5 μL of the suspension is added to 100 μL of sample or standard containing TNT. TNT in the sample displaces some of the fluorescent TNT. Step 4. An aliquot is injected into the flow cytometer, which measured the fluorescence of the individual beads as they pass by the detector. a) The figure…
1. The production of arginine is terminated by the presence of excess arginine. State which phenomenon is responsible for this outcome. Explain the phenomenon in brief. 2. Suppose, a group of scientists developed a recombinant enzyme having mutations in its active site. Do you think, the activity of an enzyme would differ from the wild type? 3. Which part of the cell has a model structure of a fluid mosaic? Why do you think this happens?
Pls explain whyWhich of the following reagents is CORRECTLY matched to its purpose?a. Coomassie Brilliant Blue R250 : monitor the progress of the electrophoretic run.b. TEMED : source of free radicals that initiate the polymerization reaction. c. ethanol and glacial acetic acid : protect the gel from oxidation and dehydrationd. hydrochloric acid : source of chloride ions for the sandwich effectA 15% w/v acrylamide solution was used for a mixture of 24, 70, 95, 130 kDa proteins, instead of the recommended concentration. The resolution will _______ and the electrophoretic mobilities will _______.a. increase, increaseb. increase, decreasec. decrease, increased. decrease, decrease
1. If an agent that disrupts microtubules activity was added to cells at M phase, the cells will be expected to get arrested at ___. 2. The loss of an acidic hydrogen in a carboxyl group will lead to the formation of a/an ___. 3. Samples from a batch of cells in culture were observed to have two nuclei per cell. What could have brought about this condition? 4. A cell that does not respond to an appropriate extracellular signal from its associated environment after it has received the signal may be affected with damage to ___. 5. The ___ checkpoint permits cell entry into mitosis only if all DNA is replicated. 6. The number of which subatomic particle provides the identity of an atom? 7. What phenomenon allows the formation of double and triple bonds between carbon atoms?
39. Cell harvesting includes the following, EXCEPT: А. Application of colchicine В. Spreading of metaphase chromosomes C. Fixation D. Sterility using microbial inhibitors
One of the biggest drawbacks of cell culture is the amount of time and materials required to produce a little amount of tissue.Make a suggestion on how you might get around this restriction.