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- 6 (a). From the following diagram, what would the name of the newly created dissacharride be? TYPE ANSWER IN LOWER CASE * CHOH CHOH HO OH OH, HO OH он OH ОнWhat type of growth is displayed in the graph?Identify the texture, transparency, color, colony size, form, margin and elevation of the two colonies in the photo (a) and (b)
- 1(a) 1. What is the theory upon which intermittent sterilization is based? (B) Is dry or moist heat more efficient as a sterilizing agent? Why? (C) When would it be necessary to use aseptic techniques when inoculating a tube of nutrient broth with dirt? (D)Why do some microorganisms characteristically form a growth of the pellicle type? What environmental factors might alter the formation of a pellicle? (This is not a graded assignment)3) If Hybridoma cells are cultured supported in an agarose matrix in which nutrients and oxygen are supplied through thin equi-distanced parallel tubes that pass longitudinally along the agarose tissue bundle, name two appropriate boundary conditions to solve for maximum distance apart. Be sure to define what all the symbols and include a drawing.4) Draw a typical bacterial growth curve. Label the stages of growth and the axis of the graph.
- The average size of cells during the exponential phase of growth is... A) Less than the lag phase B) Same as delay phase C) ConstantAll of the following are valid measurements of growth except: A. Counting viable cells B. Estimating the weight of cells C. Determining the dry weight of cells D. Streaking for single colonies E. Measuring some cellular component F. Counting the total number of cellsThe death of Geobacillus stearothermophilus spores is routinely used to assess the effectiveness of sterilization procedures. In this procedure, endospores are exposed to the sterilization process (e.g., autoclaving) and then plated on germination medium. If sterilization has been successful, no growth occurs. This requires about 2 days. Yung and Ponce have developed a new approach that yields results within 15 minutes after sterilization. Their technique is based on the release of dipicolinic acid (DPA) from the spore coat by germinating cells. DPA release is monitored microscopically after endospores are placed in media containing alanine and terbium ion (Tb31). Alanine triggers germination and Tb31 binds DPA, which then fluoresces green when illuminated with UV light. Design an experiment in which this new method could be shown to be as reliable as the culturebased approach. What other sterilization procedures besides autoclaving could this technique be used to monitor?