10. Assume you have the following stock solutions: 10% sodium dodecyl sulphate (SDS) V'S M NaOH Perform the calculations and state how you would make 10 mL of lysis buffer, which has the following composition in a single tube: 1% sodium dodecyl sulphate (SDS) 0.2 M NaOH
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Dilutions of any solutions can be calculated using the formula:
C1V1 = C2V2
Where,
C1= concentration of stock solution
V1= volume of stock solution needed
C2= concentration of final solution
V2= volume of final solution
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- PROBLEM 12.27 (a) Use Table 12.5 to calculate the osmotic pressure of the hemodi- alysis solution at 25 °C. (b) If the osmotic pressure of blood at 25 °C is 7.70 atm, what is the direction of solvent movement across the semipermeable mem brane in dialysis? (Blood to dialysis solution or dialysis solution to blood)Calculate the elution volume of blue dextran (blue), myoglobin (red), and Bromocresol purple (purple) in the gel filtration chromatography below with a flow rate of 2.5 mL/min. (Take note that the black colored test tubes represent colorless eluents)An IV infusion is to contain 40 mEq of potassium ion and 10 mEq of sodium ion in 500 mL of D5W.Using a 20% w/v potassium chloride injection (MW 74.5, valence of 1) and 0.45% sodium chlorideinjection (MW 58.5, valence of 1), how many milliliters of each should be added to the 500mL bag ofD5W to supply the required ions?
- After three minutes, the concentration of drug Zip in the red blood cells is 10 mmoles l-1. What is the average rate of entry of drug Zip into the red blood cells in units of moles min-1 red blood cell-1 during the first three minutes after placing the red blood cells into the bathing solution? Assume that each red blood cell occupies about 1 x 10-13 liters.49 A 1 litre infusion of sodium chloride 0.9% is being given at a rate of 28 drops/min (standard giving set). How long will the infusion run at the specified rate?10:23 14. Assume you have the following stock solutions: 1 M Tris-HCl (pH 8.0) 0.5 M EDTA (pH 8.0) LTE Perform the calculations and state how would you make 30 mL of TE buffer, which has the following in a single tube: 10 mM Tris-HCl (pH8.0) 1 mM EDTA X
- 2 if there is a stock solution of 2000 (ug/ml) , is it possible to use SERIAL DILUTION to generate the following 8 tubes with their concentrations? A 2000 (ug/ml) B 1500 (ug/ml) C 1000 (ug/ml) D 750 (ug/ml) E 500 (ug/ml) F 250 (ug/ml) G 125 (ug/ml) H 0 (ug/ml)20 mg active ingredient in 200ml normal saline. What is the weight/ volume concentration?Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength.
- 1. 2 3. 4. 5. Study the following experimental method: • Enzyme solution: Alkaline phosphatase in 50mM Tris-HCl, pH=10 Buffer solution: 50mM Tris-HCl, pH=10 • Vitamin C • Acid Molybdate Method Prepare the 2 following test tubes: Add 100μl of the enzyme solution to 500μl of the horn sample. Add 2.4ml of the buffer solution and mix the solution. Add 100μl of the buffer solution to 500μl of the horn sample. Add 2.4ml of the buffer solution and mix the solution. Add 1ml Vitamin C, 1ml Acid Molybdate and 5ml water to the 2 tubes and mix. Leave the 2 tubes at room temperature for 20 minutes and read the absorbance at 670nm. a. b. Determination of the organophosphate concentration of a horn sample. A standard curve of phosphate will be provided. Determine the concentration of the organophosphate in the horn sample. Which of the following reagents will you include in the blank (negative control) for this experiment? Alkaline phosphatase O Buffer solution: 50mM Tris-HCl, pH=10 Vitamin C Acid…Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength. Answer: You did not give the correct unit. The correct answer is: 333 mL XA 99Mo/99mTc generator is elute Monday at 7 am, producing 1.8Ci of 99mTc inthe eluate vial, in 20ml saline. What volume of eluate should be withdrawn fromthe eluate vial into a syringe to inject a patient with 20mCi of Tc-99m at3pm?