1. What is the principle behind the DNA extraction? 2. Describe the features isolated DNA of banana.
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1. What is the principle behind the DNA extraction?
2. Describe the features isolated DNA of banana.
Step by step
Solved in 5 steps
- 2. Make simple steps to extract isolating DNA from strawberries and yeast.1. How does PFGE separate larger fragments more efficiently than standard electrophoresis? 2. Why is SYBR green less toxic than EtBr? 3. What are the similarities and differences between Manual and Automated Sanger Sequencing? 4. What is the relationship between DNA fragment length and the distance it will run in a gel? (Restriction Enzyme Digestion)1. What is the function of the DNA polymerase enzyme in the PCR? 2. What natural process is PCR based on?
- Explain why a solid state fermentation system is optimal for extracellular enzyme production from fungi? 7. You have discovered a secondary metabolite made by an unusual fungus that has great industrial potential. Unfortunately, the fungus makes very of this product, so you must velop a scheme to increase the yield. Can you think of using a technique that might be useful to enhance the productivity of that secondary metabolite?4. What is the name of the process by which bacteria pick up a different organism’s geneticmaterial?5. Genetically, how does the original bacterial DNA (plasmid) differ from the final bacterial DNAmolecule? (Do not say, “It is longer.”)6. Tetracycline is an antibiotic that is prescribed to kill bacterial infections. The transformedbacterial cell that you created has the tetracycline resistant gene in it. If this cell is placed on agrowth medium with tetracycline, will the bacteria grow or die? Explain.6. Explain why a positive test for COVID19 would appear sooner than a negative result when using real- time PCR to test if someone is infected. 7. Explain why the same primers (GMM) could detect several types of genetic modifications to common crop plants, rather than one specific gene or genetic modification. 8. Explain the general procedure for using the NanoDrop machine in analyzing DNA samples. Be sure to explain the purposes of the blanks (pure water), and of the importance of the 260/280 ratio. Identify an ideal 260/280 ratio of purified DNA.
- 1. How do we describe the two strands of DNA, once they have separated during denaturation? 2. Polymerase Chain Reaction requires both forward and reverse primers. Where do each of these primers bind? 3. What is the purpose of the primers in a PCR reaction? 4. PCR separates molecules using what property of DNA? Based on this, which direction do DNA molecules travel through a gel? (to the positive end? Or the negative end?) 5. What does ‘qPCR’ stand for and how is it different from regular PCR?1.Briefly explain the rationales of adding chemicals that can affect DNA stability in a polymerase chain reaction (PCR). 2.Why DNA melting is required in PCR? Briefly explain how PCR can be used to detect DNA mutation.1. Upon the banana DNA extraction, what do you see in the top portion of the liquid when you look at your container? 2. Is the DNA you extracted pure? What are the possible impurities? 3. What can we do with the DNA once we have purified it? Discuss different techniques and technologies associated with this.
- 14. Why denaturation stage is important in PCR? To answer the question, please explain: 3.11. Recombinant DNA Technology 189 I) what is the basic principle of PCR? 2) what are the components of PCR? 3) what are the main stages of PCR?1. Compare and contrast the considerations in terms of methods and requirements in introducing recombinant DNA in a) plant calli and b) animal cel line. Answer and explain this comprehensively.1. What is the purpose of restriction enzyme? 2. What is the purpose of the probe in DNA finger printing. 3. What is/are the sample that can be used for DNA finger printing? 4. Who is the culprit base on our interactive laboratory experiment?