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1. What are the enzymes that are being tested in a litmus milk medium? Answer this comprehensively and please, do not just copy for somewhere.
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- Ahmad has prepared a seed culture containing recombinant Escherichia coli for the production of lipase enzyme. The seed culture was incubated for 18 hours before inoculating 5% of the seed culture into 2L of production media. a. Predict the consequences if the seed culture has been contaminated. b. Discuss whether the seed culture is meeting the criteria as inoculum or not. asap please.Copy and paste the link below and watch the video on Youtube https://www.youtube.com/watch?v=8RBs0Ghg_48 Answer the following Questions: 1. What are the chemicals and materials used in gel electrophoresis? 2. Draw a schematic diagram of a gel electrophoresis set-up 3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam? 4. What is the use of ethidium bromide and why must you wear gloves when you handle it? 5. What makes the DNA fragment move towards the positive plate? 6. What is the purpose of glycerol in the sample buffer? 7. What is the use of a DNA ladder? 8. What will happen when you increase the voltage of the set-up? 9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?Explain the mechanism of molt inhibiting hormone (MIH) and provide the evidence or articles for your explanation (minimum 5). Don't copy and paste. Thanks in advance.
- MEDIA. Match the name of the medium with the physiological test. A medium may be used more than once. Tests may require more than one answer. a. Kligler’s iron agar b. MR-VP broth c. phenol red lactose d. SIM medium e. Simmon’s citrate agar f. skim milk agar g. spirit blue agar h. tryptone broth 2,3-butanediol fermentation carbohydrate fermentation casein hydrolysis citrate utilization hydrogen sulfide production mixed-acid fermentation triglyceride hydrolysis tryptophan degradation1. What is the significance of the formation of the colored product in the oxidase test? Answer this comprehensively and please, do not just copy from somewhere.Which of the following is a reason to run simultaneous tests on known positive and negative controls when identifying an unknown microbe using biochemical tests? O The controls maintain the differential qualities of the test medium The controls will ensure that the unknown microbe multiplies on the medium O The controls can show if there was contamination of the tubes with another species O The controls can show if there was a recent mutation in your unknown microbe MacBook Air DII F12 80 888 F7 F5 F4 F3 * & %2$ %23 6 7 8. 4 5
- 3) Define gel electrophoresis, including its theory and application. Describe the steps of running gel electrophoresis using the following image. More detailed reading: https://www.sciencedirect.com/topics/medicine-and-dentistry/agar-gel-electrophoresis POWER SUPPLY CATHODE ELECTROPHORETIC BUFFER ANODE WELL SAMPLE AGAROSE GEL POWER SUPPLY CATHODE ANOCE HIGH MOLECULAR WEIGHT SPECIES LOW MOLECULAR WEIGHT ANALYTES1. What results are expected if you drop H2O2 into a cut on your arm? Explain why those results are expected. 2. To do this test, why do you have to grow the organism in medium containing tryptophan? 3. Why is a red color after the addition of zinc a negative result for nitrate reductase?ANSWER THE FOLLOWING QUESTIONS REGARDING GEL ELECTROPHORESIS 1.why is it not advisable to move/touch the agarose gel in the process of hardening 2.what is the use or function of the TAE buffer that is poured or found in the gel box 3.how can you tell if agel is running 4.outline the process use in the preparation of agarose gel of 1.5 concentration 5.outline the processes/steps used in gel electrophoresis 6.name twoexamples of the dye used in gel electrophoresis 7.how do we prepare x1(concentration) TAE BUFFER form 50x TAE buffer
- THIS IS A 2 PART Question a. Is this a good streak? If so, explain why. If not, explain what you would change. b. Is this streak of a pure culture, a mixed culture, or a contaminated culture? Explain your response.ascocarps from along the petri dish perimeter. Leave this Sordaria cross plate out of direct sunlight at 22-24 degrees C for 8-10 days. After this incubation the mature Sordaria cross plate cultures will look like the image on the right above. Use a toothpick or sterile loop to remove several ascocarps (see page 2 of the pre-lab file) from one of the hybrid zones. Make a wet mount of the ascocarps on a microscope slide and gently press the coverslip with your thumb or the eraser on the end of a pencil until the ascocarps are broken open. This will release clusters of asci. Place the slide under the microscope and scan the slide looking for clusters of released asci. Identify hybrid asci (with both dark and tan spores). Once you have found a cluster of hybrid asci score as many asci as possible for the arrangement of dark and light spores looking for asci where crossing over has taken place (see pre lab file) and below: No crossing over: Crossing over: For the asci shown in the figure…Tables Table 1: Time Required for Methylene Blue Color Change Milk Sample Start Time/Date (Step 10) End Time/Date (Step 11) Time Elapsed (End Time-Start Time) 0 hours 12:00PM 2/14/2020 12:30PM 12/14/2020 30 minutes 1 hour 12.00 PM 12/14/2020 1:30PM 12/14/2020 1 hour 3 hours 12:00PM 12/14/2020 3:00PM 12/14/2020 3 hours 4 hours 12:00PM 12/14/2020 12:00PM 4:00PM 4 hours Which sample took the least time to become white? Why do you think this was the case?