1. For each of the following, give a brief description of the purpose and give an application a. Restriction enzymes b. Plasmids c. Gel Electrophoresis
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- Demonstrate a procedure that you could adopt to restrict or digest the TOL plasmid.10. When looking at your agar plate, where are the bacteria that did not take up the plasmid during the transformation? (It is a 4 letter word). Thanks for the help!Write the advantages and disadvantages of applying such application in the DNA of an organism. 2. Drug delivery systems that are based on bacterial or viral host.
- Define/Compare/Contrast/Distinguish these three terms: F plasmid F’ plasmid (and how is it formed) Hfr cell (and how is it formed)1. How large is your plasmid 2. Which colony(-ies) is(are) likely to have been white? 3. Which colony(-ies) is (are) likely to have the XRAY gene? asap pleaseexplain the steps for restriction and how each procedure of these three routes is performed in rabbits: 1.SQ 2.IM 3.IV
- explain F' plasmid formation4. Complete the table below. CONJUGATION TRANSFORMATION TRANSDUCTION DEFINITION PROPONENTS/DISCOVERER MECHANISM OF DNA ΕΝTRY ΤΟ ΤHE RECIPIENT BACTERIAL CELL REQUIERMENT OF A VIRAL HOST PLASMID TRANSFER VIRAL LIFE CYCLE INVOLVED ACQUIRING ΑΝΤΙΒΙΟTIC RESISTANCEGive advantages and disadvantages of using commercially available kits for plasmid extraction over conventional alkaline lysis technique.
- Identify a plasmid that is used to destroy its competitors. fertility plasmids bacteriocin plasmids virulence plasmids cryptic plasmids resistance plasmidsexplain F' plasmid formation diagramatically1. Identify the 2 CORRECT statements from the following. Mark both answers Group of answer choices bacterial chromosomes are associated with histone proteins resistance plasmid is a self transmissible plasmid bacterial ribosomes consist of 3 subunits Bacillus anthracis plasmid codes for the capsule plasmid presence are essential for bacterial survival